Patents by Inventor Mark Saltzman

Mark Saltzman has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Publication number: 20170283830
    Abstract: Compositions and methods for enhancing targeted gene editing and methods of use thereof are disclosed. In the most preferred embodiments, gene editing is carried out utilizing a gene editing composition such as triplex-forming oligonucleotides, CRISPR, zinc finger nucleases, TALENS, or others, in combination with a gene modification potentiating agent such as stem cell factor (SCF), a CHK1 or ATR inhibitor, or a combination thereof. A particular preferred gene editing composition is triplex-forming peptide nucleic acids (PNAs) substituted at the ? position for increased DNA binding affinity. Nanoparticle compositions for intracellular delivery of the gene editing composition are also provided and particular advantageous for use with in vivo applications.
    Type: Application
    Filed: February 16, 2017
    Publication date: October 5, 2017
    Inventors: W. Mark Saltzman, Peter Glazer, Raman Bahal, Nicole Ali McNeer, Danith H. Ly, Elias Quijano
  • Publication number: 20170121454
    Abstract: Poly(amine-co-ester) polymers, methods of forming active agent-load nanoparticles therefrom, and methods of using the nanoparticles for drug delivery are disclosed. The nanoparticles can be coated with an agent that reduces surface charge, an agent that increases cell-specific targeting, or a combination thereof. Typically, the loaded nanoparticles are less toxic, more efficient at drug delivery, or a combination thereof compared to a control other transfection reagents. In some embodiments, the nanoparticles are suitable for in vivo delivery, and can be administered systemically to a subject to treat a disease or condition.
    Type: Application
    Filed: October 24, 2016
    Publication date: May 4, 2017
    Inventors: W. MARK SALTZMAN, ZHAOZHONG JIANG, JIANGBING ZHOU, JIE LIU
  • Publication number: 20170043751
    Abstract: A wheel chock having a wedge-shaped body and a recessed gripping member is provided. The wedge-shaped body includes a bottom surface for engaging the ground, a front inclined wheel engaging surface, a back surface opposite the front inclined surface, and opposite upright side surfaces. At least one of the opposite upright side surfaces has a recess, and the gripping member is disposed in the recess. The gripping member does not substantially protrude beyond the upright side surface having the recess for enabling an improvement in compact packaging and shipping costs associated with the wheel chock.
    Type: Application
    Filed: August 11, 2016
    Publication date: February 16, 2017
    Inventors: Mark Saltzman, Dave Zelis, Scott Moorman
  • Patent number: 9567430
    Abstract: Poly(amine-co-ester) polymers, methods of forming active agent-load nanoparticles therefrom, and methods of using the nanoparticles for drug delivery are disclosed. The nanoparticles can be coated with an agent that reduces surface charge, an agent that increases cell-specific targeting, or a combination thereof. Typically, the loaded nanoparticles are less toxic, more efficient at drug delivery, or a combination thereof compared to a control other transfection reagents. In some embodiments, the nanoparticles are suitable for in vivo delivery, and can be administered systemically to a subject to treat a disease or condition.
    Type: Grant
    Filed: January 5, 2016
    Date of Patent: February 14, 2017
    Assignee: Yale University
    Inventors: W. Mark Saltzman, Zhaozhong Jiang, Jiangbing Zhou, Jie Liu
  • Publication number: 20160251478
    Abstract: Poly(amine-co-ester) polymers, methods of forming active agent-load nanoparticles therefrom, and methods of using the nanoparticles for drug delivery are disclosed. The nanoparticles can be coated with an agent that reduces surface charge, an agent that increases cell-specific targeting, or a combination thereof. Typically, the loaded nanoparticles are less toxic, more efficient at drug delivery, or a combination thereof compared to a control other transfection reagents. In some embodiments, the nanoparticles are suitable for in vivo delivery, and can be administered systemically to a subject to treat a disease or condition.
    Type: Application
    Filed: January 5, 2016
    Publication date: September 1, 2016
    Inventors: W. MARK SALTZMAN, ZHAOZHONG JIANG, JIANGBING ZHOU, JIE LIU
  • Publication number: 20160251477
    Abstract: Polymers including poly(amine-co-ester), poly(amine-co-amide), or a combination thereof, and nanoparticles, particularly solid core nanoparticles, formed therefrom are provided. Solid core nanoparticles fabricated from hydrophobic polymers often require the presence of cationic complexing agents to stabilize negatively charged active agents such as siRNA. However, complexing agents are optional in the disclosed formulations because the nanoparticles contain cationic amines to stabilize negatively charged nucleic acids and hydrophobic domains to condense the nucleic acid into the core of the formed nanoparticles, thus improving encapsulation efficiency. This increase in nucleic acid loading allows the disclosed solid core nanoparticles to deliver more nucleic acid per cell without increasing total polymer delivered, further reducing cytotoxicity.
    Type: Application
    Filed: May 11, 2016
    Publication date: September 1, 2016
    Inventors: Jiajia Cui, Junwei Zhang, W. Mark Saltzman
  • Publication number: 20160152987
    Abstract: Polymeric nanoparticles encapsulating inhibitory ribonucleic acids (RNAs) and methods of their manufacture and use are provided. Advantageous properties of the nanoparticles include: 1) high encapsulation efficiency of inhibitory RNAs into the nano articles, 2) small size of the nanoparticles that increases cell internalization, and 3) sustained release of encapsulated inhibitory RNAs by the nanoparticles that allows for administration of an effective amount of inhibitory RNAs to cells or tissues over extended periods of time. Encapsulation efficiency of inhibitory RNAs into the nanoparticles is greatly increased by complexing the inhibitory RNAs to polycations prior to encapsulation. Methods of using the polymeric nanoparticles for treating or inhibiting diseases or disorders are provided.
    Type: Application
    Filed: December 21, 2015
    Publication date: June 2, 2016
    Inventors: W. Mark Saltzman, Kim Woodrow
  • Patent number: 9272043
    Abstract: Poly(amine-co-ester) polymers, methods of forming active agent-load nanoparticles therefrom, and methods of using the nanoparticles for drug delivery are disclosed. The nanoparticles can be coated with an agent that reduces surface charge, an agent that increases cell-specific targeting, or a combination thereof. Typically, the loaded nanoparticles are less toxic, more efficient at drug delivery, or a combination thereof compared to a control other transfection reagents. In some embodiments, the nanoparticles are suitable for in vivo delivery, and can be administered systemically to a subject to treat a disease or condition.
    Type: Grant
    Filed: June 2, 2014
    Date of Patent: March 1, 2016
    Assignee: Yale University
    Inventors: W. Mark Saltzman, Zhaozhong Jiang, Jiangbing Zhou, Jie Liu
  • Patent number: 9241898
    Abstract: Polymeric nanoparticles encapsulating inhibitory ribonucleic acids (RNAs) and methods of their manufacture and use are provided. Advantageous properties of the nanoparticles include: 1) high encapsulation efficiency of inhibitory RNAs into the nanoparticles, 2) small size of the nanoparticles that increases cell internalization, and 3) sustained release of encapsulated inhibitory RNAs by the nanoparticles that allows for administration of an effective amount of inhibitory RNAs to cells or tissues over extended periods of time. Encapsulation efficiency of inhibitory RNAs into the nanoparticles is greatly increased by complexing the inhibitory RNAs to polycations prior to encapsulation. Methods of using the polymeric nanoparticles for treating or inhibiting diseases or disorders are provided.
    Type: Grant
    Filed: March 11, 2009
    Date of Patent: January 26, 2016
    Assignee: Yale University
    Inventors: W. Mark Saltzman, Kim Woodrow
  • Publication number: 20150125384
    Abstract: Modular nanoparticle vaccine compositions and methods of making and using the same have been developed. Modular nanoparticle vaccine compositions comprise an antigen encapsulated in a polymeric particle and adaptor elements which modularly couple functional elements to the particle. The modular design of these vaccine compositions, which involves flexible addition and subtraction of antigen, adjuvant, immune potentiators, molecular recognition and transport mediation elements, as well as intracellular uptake mediators, allows for exquisite control over variables that are important in optimizing an effective vaccine delivery system.
    Type: Application
    Filed: November 10, 2014
    Publication date: May 7, 2015
    Inventors: Ira S. Mellman, Tarek M. Fahmy, William Mark Saltzman, Michael J. Caplan
  • Publication number: 20150118311
    Abstract: Brain-penetrating polymeric nanoparticles that can be loaded with drugs and are optimized for intracranial convection-enhanced delivery (CED) have been developed. In the preferred embodiment, these are loaded with FDA-approved compounds, identified through library screening to target brain cancer stem cells (BSCSs). The particles are formed by emulsifying a polymer-drug solution, then removing solvent and centrifuging at a first force to remove the larger particles, then collecting the smaller particles using a second higher force to sediment the smaller particles having a diameter of less than 100 nm, more preferably less than 90 nanometers average diameter, able to penetrate brain interstitial spaces.
    Type: Application
    Filed: May 6, 2013
    Publication date: April 30, 2015
    Applicant: Yale Universit
    Inventors: Jiangbing Zhou, Toral R. Patel, Joseph M. Piepmeier, William Mark Saltzman
  • Publication number: 20150073041
    Abstract: Polyamine-co-ester-co-ortho ester) polymers, methods of forming active agent-load nanoparticles therefrom, and methods of using the nanoparticles for drug delivery are disclosed. The nanoparticles can be coated with an agent that reduces surface charge, an agent that increases cell-specific targeting, or a combination thereof. Typically, the loaded nanoparticles are less toxic, more efficient at drug delivery, or a combination thereof compared to a control or other transfection reagents.
    Type: Application
    Filed: November 18, 2014
    Publication date: March 12, 2015
    Inventors: W. Mark Saltzman, Junwei Zhang, Jiangbing Zhou, Zhaozhong Jiang
  • Publication number: 20140371712
    Abstract: An embodiment of the invention is directed to a microfabricated, silicon-based, Convection Enhanced Delivery (CED) device. The device comprises a silicon shank portion, at least one individual parylene channel disposed along at least a part of an entire length of the shank, wherein the channel has one or more dimensioned fluid exit ports disposed at one or more respective locations of the channel and a fluid (drug) input opening. The fluid input opening may be configured or adapted to be connected to a fluid reservoir and/or a pump and/or a meter and/or a valve or other suitable control device(s) or apparatus that supplies and/or delivers fluid (e.g., a drug) to the microfabricated device. The device may have multiple channels disposed side by side or in different surfaces of the device. The device may be rigid, or flexible, in which case a flexible device can be attached to a bio-degradable support scaffold that provides sufficient structural rigidity for insertion of the device into the target tissue.
    Type: Application
    Filed: June 25, 2014
    Publication date: December 18, 2014
    Applicants: YALE UNIVERSITY, CORNELL UNIVERSITY
    Inventors: William L. Olbricht, Keith B. Neeves, Conor Foley, Russell T. Matthews, W. Mark Saltzman, Andrew Sawyer
  • Publication number: 20140342003
    Abstract: Poly(amine-co-ester) polymers, methods of forming active agent-load nanoparticles therefrom, and methods of using the nanoparticles for drug delivery are disclosed. The nanoparticles can be coated with an agent that reduces surface charge, an agent that increases cell-specific targeting, or a combination thereof. Typically, the loaded nanoparticles are less toxic, more efficient at drug delivery, or a combination thereof compared to a control other transfection reagents. In some embodiments, the nanoparticles are suitable for in vivo delivery, and can be administered systemically to a subject to treat a disease or condition.
    Type: Application
    Filed: June 2, 2014
    Publication date: November 20, 2014
    Inventors: W. Mark Saltzman, Zhaozhong Jiang, Jiangbing Zhou
  • Patent number: 8889117
    Abstract: Modular nanoparticle vaccine compositions and methods of making and using the same have been developed. Modular nanoparticle vaccine compositions comprise an antigen encapsulated in a polymeric particle and adaptor elements which modularly couple functional elements to the particle. The modular design of these vaccine compositions, which involves flexible addition and subtraction of antigen, adjuvant, immune potentiators, molecular recognition and transport mediation elements, as well as intracellular uptake mediators, allows for exquisite control over variables that are important in optimizing an effective vaccine delivery system.
    Type: Grant
    Filed: February 15, 2008
    Date of Patent: November 18, 2014
    Assignee: Yale University
    Inventors: Ira S. Mellman, Tarek M. Fahmy, William Mark Saltzman, Michael J. Caplan
  • Patent number: 8790317
    Abstract: An embodiment of the invention is directed to a microfabricated, silicon-based, Convection Enhanced Delivery (CED) device. The device comprises a silicon shank portion, at least one individual parylene channel disposed along at least a part of an entire length of the shank, wherein the channel has one or more dimensioned fluid exit ports disposed at one or more respective locations of the channel and a fluid (drug) input opening. The fluid input opening may be configured or adapted to be connected to a fluid reservoir and/or a pump and/or a meter and/or a valve or other suitable control device(s) or apparatus that supplies and/or delivers fluid (eg, a drug) to the microfabricated device. The device may have multiple channels disposed side by side or in different surfaces of the device.
    Type: Grant
    Filed: February 12, 2008
    Date of Patent: July 29, 2014
    Assignees: Cornell University, Yale University
    Inventors: William L. Olbricht, Keith B. Neeves, Conor Foley, Russell T. Mattews, W. Mark Saltzman, Andrew Sawyer
  • Publication number: 20110268810
    Abstract: Polymeric microparticles are used to deliver recombinagenic or mutagenic nucleic acid molecules such as donor nucleic acid alone, or in combination with triplex-forming molecules, to induce a site-specific mutation in the target DNA. Target cells endocytose the particles, releasing the nucleic acid molecules inside of the cell, where they induce mutagenesis or recombination at a target site. The examples demonstrate that triplex forming oligonucleotides, preferably PNAs, preferably in combination with a donor nucleotide molecule, can be encapsulated into polymeric microparticles, which are delivered into cells. Results demonstrate significantly greatly levels of uptake and expression, and less cytotoxicity, as compared to direct transfer of the nucleic acid molecules into the cell by nucleofection.
    Type: Application
    Filed: November 2, 2010
    Publication date: November 3, 2011
    Inventors: William Mark Saltzman, Peter M. Glazer, Joanna Chin, Nicole McNeer
  • Publication number: 20100151436
    Abstract: Methods for ex vivo administration of drugs to grafts using polymeric micro- and nanoparticles and applications for these methods are described herein. The particles contain encapsulated molecules which are released locally at the site of implantation and function to prevent graft rejection or aid in the proper adaptation of the graft to the host. The disclosed methods may be used to inhibit or reduce hyperplasia and stenosis of vascular grafts or to prevent graft rejection.
    Type: Application
    Filed: February 28, 2008
    Publication date: June 17, 2010
    Inventors: Peter M. Fong, William Mark Saltzman, Tarek M. Fahmy
  • Publication number: 20100104503
    Abstract: Modular nanoparticle vaccine compositions and methods of making and using the same have been developed. Modular nanoparticle vaccine compositions comprise an antigen encapsulated in a polymeric particle and adaptor elements which modularly couple functional elements to the particle. The modular design of these vaccine compositions, which involves flexible addition and subtraction of antigen, adjuvant, immune potentiators, molecular recognition and transport mediation elements, as well as intracellular uptake mediators, allows for exquisite control over variables that are important in optimizing an effective vaccine delivery system.
    Type: Application
    Filed: February 15, 2008
    Publication date: April 29, 2010
    Inventors: Ira S. Mellman, Tarek M. Fahmy, William Mark Saltzman, Michael J. Caplan
  • Publication number: 20090269397
    Abstract: Polymeric delivery devices have been developed which combine high loading/high density of molecules to be delivered with the option of targeting. As used herein, “high density” refers to microparticles having a high density of ligands or coupling agents, which is in the range of 1000-10,000,000, more preferably between 10,000 and 1,000,000 ligands per square micron of microparticle surface area. A general method for incorporating molecules into the surface of biocompatible polymers using materials with an HLB of less than 10, more preferably less than 5, such as fatty acids, has been developed. Because of its ease, generality and flexibility, this method has widespread utility in modifying the surface of polymeric materials for applications in drug delivery and tissue engineering, as well other fields.
    Type: Application
    Filed: May 18, 2009
    Publication date: October 29, 2009
    Inventors: William Mark Saltzman, Tarek Fahmy, Peter Fong