Patents by Inventor Mark Skylar-Scott
Mark Skylar-Scott has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Patent number: 11969935Abstract: In one aspect, the present disclosure provides a nozzle for a 3D printing system. The nozzle may include a flowpath with a material inlet and a material outlet. The nozzle may further include a valve in fluid communication with the flowpath between the material inlet and the material outlet, where the valve includes a closed state and an open state, where in the closed state the valve obstructs the flowpath between the material inlet and the material outlet, and where in the open state the material inlet is in fluid communication with the material outlet. The nozzle may further include a compensator in fluid communication with the flowpath, where the compensator includes a contracted state associated with the open state of the valve and an expanded state associated with the closed state of the valve.Type: GrantFiled: December 5, 2017Date of Patent: April 30, 2024Assignee: PRESIDENT AND FELLOWS OF HARVARD COLLEGEInventors: Jennifer A. Lewis, Mark A. Skylar-Scott, Jochen Mueller
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Publication number: 20240100767Abstract: In one aspect, the present disclosure provides a nozzle for a 3D printing system. The nozzle may include a flowpath with a material inlet and a material outlet. The nozzle may further include a valve in fluid communication with the flowpath between the material inlet and the material outlet, where the valve includes a closed state and an open state, where in the closed state the valve obstructs the flowpath between the material inlet and the material outlet, and where in the open state the material inlet is in fluid communication with the material outlet. The nozzle may further include a compensator in fluid communication with the flowpath, where the compensator includes a contracted state associated with the open state of the valve and an expanded state associated with the closed state of the valve.Type: ApplicationFiled: September 6, 2023Publication date: March 28, 2024Applicant: President and Fellows of Harvard CollegeInventors: Jennifer A. LEWIS, Mark A. Skylar-Scott, Jochen Mueller
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Patent number: 11648106Abstract: This disclosure features artificial tympanic membrane graft devices and two-component bilayer graft devices that include a scaffold having a plurality of ribs made of a first material and a plurality of spaces between the ribs filled or made with the first material, a different, second material, a combination of the first and a second materials, or a combination of a second material and one or more other different materials. The bilayer graft devices have two components or layers. One component, e.g., the underlay graft device, can include a projection, and the second component, e.g., the overlay graft device, can include an opening that corresponds to the projection (or vice versa) so that the opening and the projection can secure the two layers together in a “lock and key” manner. This disclosure also features methods of making, using, and implanting the three-dimensional artificial tympanic membrane and bilayer graft devices.Type: GrantFiled: September 22, 2020Date of Patent: May 16, 2023Assignees: Massachusetts Eye and Ear Infirmary, President and Fellows of Harvard CollegeInventors: Aaron K. Remenschneider, Elliott Kozin, Nicole Leah Black, Michael J. McKenna, Daniel J. Lee, Jennifer A. Lewis, John Rosowski, David B. Kolesky, Mark A. Skylar-Scott, Alexander D. Valentine
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Patent number: 11214768Abstract: Methods of tissue engineering, and more particularly methods and compositions for generating various vascularized 3D tissues, such as 3D vascularized embryoid bodies and organoids are described. Certain embodiments relate to a method of generating functional human tissue, the method comprising embedding an embryoid body or organoid in a tissue construct comprising a first vascular network and a second vascular network, each vascular network comprising one or more interconnected vascular channels; exposing the embryoid body or organoid to one or more biological agents, a biological agent gradient, a pressure, and/or an oxygen tension gradient, thereby inducing angiogenesis of capillary vessels to and/or from the embryoid body or organoid; and vascularizing the embryoid body or organoid, the capillary vessels connecting the first vascular network to the second vascular network, thereby creating a single vascular network and a perfusable tissue structure.Type: GrantFiled: March 3, 2016Date of Patent: January 4, 2022Assignee: PRESIDENT AND FELLOWS OF HARVARD COLLEGEInventors: Jennifer A. Lewis, Mark A. Skylar-Scott, David B. Kolesky, Kimberly A. Homan, Alex H. M. Ng, George M. Church
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Patent number: 11214661Abstract: The present invention enables three-dimensional nanofabrication by isotropic shrinking of patterned hydrogels. A hydrogel is first expanded, the rate of expansion being controlled by the concentration of the crosslinker. The hydrogel is then infused with a reactive group and patterned in three dimensions using a photon beam through a limited-diffraction microscope. Functional particles or materials are then deposited on the pattern. The hydrogel is then shrunk and cleaved from the pattern.Type: GrantFiled: September 9, 2016Date of Patent: January 4, 2022Assignee: Massachusetts Institute of TechnologyInventors: Samuel G. Rodriques, Daniel Oran, Ruixuan Gao, Shoh Asano, Mark A. Skylar-Scott, Fei Chen, Paul W. Tillberg, Adam H. Marblestone, Edward S. Boyden
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Publication number: 20210000590Abstract: This disclosure features artificial tympanic membrane graft devices and two-component bilayer graft devices that include a scaffold having a plurality of ribs made of a first material and a plurality of spaces between the ribs filled or made with the first material, a different, second material, a combination of the first and a second materials, or a combination of a second material and one or more other different materials. The bilayer graft devices have two components or layers. One component, e.g., the underlay graft device, can include a projection, and the second component, e.g., the overlay graft device, can include an opening that corresponds to the projection (or vice versa) so that the opening and the projection can secure the two layers together in a “lock and key” manner. This disclosure also features methods of making, using, and implanting the three-dimensional artificial tympanic membrane and bilayer graft devices.Type: ApplicationFiled: September 22, 2020Publication date: January 7, 2021Inventors: Aaron K. Remenschneider, Elliott Kozin, Nicole Leah Black, Michael J. McKenna, Daniel J. Lee, Jennifer A. Lewis, John Rosowski, David B. Kolesky, Mark A. Skylar-Scott, Alexander D. Valentine
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Publication number: 20200360567Abstract: A 3D printed tubular construct, such as a nephron, with or without embedded vasculature as well as methods of printing tubular tissue constructs are described.Type: ApplicationFiled: May 29, 2020Publication date: November 19, 2020Applicant: President and Fellows of Harvard CollegeInventors: Jennifer A. Lewis, Kimberly A. Homan, David B. Kolesky, Ryan L. Truby, Mark A. Skylar-Scott
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Patent number: 10786349Abstract: This disclosure features artificial tympanic membrane graft devices and two-component bilayer graft devices that include a scaffold having a plurality of ribs made of a first material and a plurality of spaces between the ribs filled or made with the first material, a different, second material, a combination of the first and a second materials, or a combination of a second material and one or more other different materials. The bilayer graft devices have two components or layers. One component, e.g., the underlay graft device, can include a projection, and the second component, e.g., the overlay graft device, can include an opening that corresponds to the projection (or vice versa) so that the opening and the projection can secure the two layers together in a “lock and key” manner. This disclosure also features methods of making, using, and implanting the three-dimensional artificial tympanic membrane and bilayer graft devices.Type: GrantFiled: March 21, 2016Date of Patent: September 29, 2020Assignees: Massachusetts Eye and Ear Infirmary, President and Fellows of Harvard CollegeInventors: Aaron K. Remenschneider, Elliot Kozin, Nicole Black, Michael J. McKenna, Daniel J. Lee, Jennifer Lewis, John Rosowski, David Kolesky, Mark A. Skylar-Scott, Alexander D. Valentine
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Publication number: 20200289709Abstract: Described are methods for producing tissue constructs, tissue constructs produced by the methods, and their use. The described method of producing a tissue construct comprises providing a granular tissue, depositing one or more filaments on or in the granular tissue, each filament comprising an ink, and gelling or fusing the granular tissue, thereby producing the tissue construct.Type: ApplicationFiled: September 20, 2018Publication date: September 17, 2020Inventors: Mark Skylar-Scott, Sebastien Uzel, Jennifer Lewis
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Publication number: 20200248147Abstract: Described are methods of enhancing development of renal organoids, methods of using the same, and kits.Type: ApplicationFiled: June 8, 2018Publication date: August 6, 2020Applicants: President and Fellows of Harvard College, The Brigham And Women's Hospital, Inc.Inventors: Kimberly A. Homan, Navin R. Gupta, Katharina T. Kroll, David B. Kolesky, Mark Skylar-Scott, Michael T. Valerius, Joseph Bonventre, Ryuji Morizane, Jennifer Lewis
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Patent number: 10702630Abstract: A 3D printed tubular construct, such as a nephron, with or without embedded vasculature as well as methods of printing tubular tissue constructs are described.Type: GrantFiled: May 4, 2016Date of Patent: July 7, 2020Assignee: PRESIDENT AND FELLOWS OF HARVARD COLLEGEInventors: Jennifer A. Lewis, Kimberly A. Homan, David B. Kolesky, Ryan L. Truby, Mark A. Skylar-Scott
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Publication number: 20200164109Abstract: Described are methods for producing multi-layered tubular tissue structures, tissue structures produced by the methods, and their use.Type: ApplicationFiled: July 20, 2018Publication date: May 28, 2020Applicant: President and Fellows of Harvard CollegeInventors: Katharina Theresa Kroll, Kimberly A. Homan, Mark A. Skylar-Scott, Sebastien G.M. Uzel, David B. Kolesky, Patrick Lustenberger, Jennifer A. Lewis
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Publication number: 20190022283Abstract: A printed tissue construct comprises one or more tissue patterns, where each tissue pattern comprises a plurality of viable cells of one or more predetermined cell types. A network of vascular channels interpenetrates the one or more tissue patterns. An extracellular matrix composition at least partially surrounds the one or more tissue patterns and the network of vascular channels. A method of printing a tissue construct with embedded vasculature comprises depositing one or more cell-laden filaments, each comprising a plurality of viable cells, on a substrate to form one or more tissue patterns. Each of the one or more tissue patterns comprises one or more predetermined cell types. One or more sacrificial filaments, each comprising a fugitive ink, are deposited on the substrate to form a vascular pattern interpenetrating the one or more tissue patterns. The vascular pattern and the one or more tissue patterns are at least partially surrounded with an extracellular matrix composition.Type: ApplicationFiled: September 26, 2018Publication date: January 24, 2019Applicant: President and Fellows of Harvard CollegeInventors: Jennifer A. LEWIS, David B. KOLESKY, Mark A. SKYLAR-SCOTT, Kimberly A. HOMAN, Ryan L. TRUBY, Amelia Sydney GLADMAN
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Patent number: 10117968Abstract: A printed tissue construct comprises one or more tissue patterns, where each tissue pattern comprises a plurality of viable cells of one or more predetermined cell types. A network of vascular channels interpenetrates the one or more tissue patterns. An extracellular matrix composition at least partially surrounds the one or more tissue patterns and the network of vascular channels. A method of printing a tissue construct with embedded vasculature comprises depositing one or more cell-laden filaments, each comprising a plurality of viable cells, on a substrate to form one or more tissue patterns. Each of the one or more tissue patterns comprises one or more predetermined cell types. One or more sacrificial filaments, each comprising a fugitive ink, are deposited on the substrate to form a vascular pattern interpenetrating the one or more tissue patterns. The vascular pattern and the one or more tissue patterns are at least partially surrounded with an extracellular matrix composition.Type: GrantFiled: November 4, 2014Date of Patent: November 6, 2018Assignee: President And Fellows Of Harvard CollegeInventors: Jennifer A. Lewis, David B. Kolesky, Mark A. Skylar-Scott, Kimberly A. Homan, Ryan L. Truby, Amelia Sydney Gladman
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Publication number: 20180110901Abstract: A 3D printed tubular construct, such as a nephron, with or without embedded vasculature as well as methods of printing tubular tissue constructs are described.Type: ApplicationFiled: May 4, 2016Publication date: April 26, 2018Applicant: President and Fellows of Harvard CollegeInventors: Jennifer A. Lewis, Kimberly A. Homan, David B. Kolesky, Ryan L. Truby, Mark A. Skylar-Scott
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Publication number: 20180042718Abstract: This disclosure features artificial tympanic membrane graft devices and two-component bilayer graft devices that include a scaffold having a plurality of ribs made of a first material and a plurality of spaces between the ribs filled or made with the first material, a different, second material, a combination of the first and a second materials, or a combination of a second material and one or more other different materials. The bilayer graft devices have two components or layers. One component, e.g., the under-lay graft device, can include a projection, and the second component, e.g., the overlay graft device, can include an opening that corresponds to the projection (or vice versa) so that the opening and the projection can secure the two layers together in a “lock and key” manner. This disclosure also features methods of making, using, and implanting the three-dimensional artificial tympanic membrane and bilayer graft devices.Type: ApplicationFiled: March 21, 2016Publication date: February 15, 2018Inventors: Aaron K. Remenschneider, Elliott Kozin, Nicole Black, Michael J. McKenna, Daniel J. Lee, Jennifer Lewis, John Rosowski, David Kolesky, Mark A. Skylar-Scott, Alexander D. Valentine
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Publication number: 20180030409Abstract: Methods of tissue engineering, and more particularly methods and compositions for generating various vascularized 3D tissues, such as 3D vascularized embryoid bodies and organoids are described. Certain embodiments relate to a method of generating functional human tissue, the method comprising embedding an embryoid body or organoid in a tissue construct comprising a first vascular network and a second vascular network, each vascular network comprising one or more interconnected vascular channels; exposing the embryoid body or organoid to one or more biological agents, a biological agent gradient, a pressure, and/or an oxygen tension gradient, thereby inducing angiogenesis of capillary vessels to and/or from the embryoid body or organoid; and vascularizing the embryoid body or organoid, the capillary vessels connecting the first vascular network to the second vascular network, thereby creating a single vascular network and a perfusable tissue structure.Type: ApplicationFiled: March 3, 2016Publication date: February 1, 2018Inventors: Jennifer A. Lewis, Mark A. Skylar-Scott, David B. Kolesky, Kimberly A. Homan, Alex H.M. Ng, George M. Church
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Publication number: 20170081489Abstract: The present invention enables three-dimensional nanofabrication by isotropic shrinking of patterned hydrogels. A hydrogel is first expanded, the rate of expansion being controlled by the concentration of the crosslinker. The hydrogel is then infused with a reactive group and patterned in three dimensions using a photon beam through a limited-diffraction microscope. Functional particles or materials are then deposited on the pattern. The hydrogel is then shrunk and cleaved from the pattern.Type: ApplicationFiled: September 9, 2016Publication date: March 23, 2017Inventors: Samuel G. Rodriques, Daniel Oran, Ruixuan Gao, Shoh Asano, Mark A. Skylar-Scott, Fei Chen, Paul W. Tillberg, Adam H. Marblestone, Edward S. Boyden
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Publication number: 20160287756Abstract: A printed tissue construct comprises one or more tissue patterns, where each tissue pattern comprises a plurality of viable cells of one or more predetermined cell types. A network of vascular channels interpenetrates the one or more tissue patterns. An extracellular matrix composition at least partially surrounds the one or more tissue patterns and the network of vascular channels. A method of printing a tissue construct with embedded vasculature comprises depositing one or more cell-laden filaments, each comprising a plurality of viable cells, on a substrate to form one or more tissue patterns. Each of the one or more tissue patterns comprises one or more predetermined cell types. One or more sacrificial filaments, each comprising a fugitive ink, are deposited on the substrate to form a vascular pattern interpenetrating the one or more tissue patterns. The vascular pattern and the one or more tissue patterns are at least partially surrounded with an extracellular matrix composition.Type: ApplicationFiled: November 4, 2014Publication date: October 6, 2016Applicant: President and Fellows of Harvard CollegeInventors: Jennifer A. Lewis, David B. Kolesky, Mark A. Skylar-Scott, Kimberly A. Homan, Ryan L. Truby, Amelia Sydney Gladman