Abstract: Certain embodiments are directed to compositions and methods for solving problems associated with measuring T:G mispairs, U:G mispairs and other 5-substituted uracil mispairs. Certain embodiments are directed to a hybrid enzyme that is capable of finding and cutting the T of the T:G mispair or other mispaired uracil analogs creating a method for their measurement. In certain embodiments the hybrid enzyme is a fusion of a human thymine DNA glycosylase (TDG) activator segment and a catalytic domain of an archaeal thermophilic thymine glycosylase (tTDG).

Abstract: A grass and leaf collection sled for riding lawn mowers. The sled vastly increases the total volume of grass, leaves, and other lawn residue that can be picked up and transported to a disposal site. The sled is easily assembled, simple to load and unload, and is easily stored after use. The sled enables collection of a large volume of lawn refuse without relying upon additional mechanical devices to complicate the operation of the lawnmower.