Abstract: An enhanced quadrature receive serial interface circuit and methods are provided for calibrating the quadrature receive serial interface circuit. A quadrature receive serial interface circuit comprises a first phase rotator and a second phase rotator generating quadrature clocks of identical frequency. Calibration of the quadrature receive serial interface circuit uses a pseudo random bit sequence (PRBS) received by the quadrature receive serial interface circuit. For calibration, one-half of the received PRBS bits are sampled and the phase rotator generating in-phase 0° and 180° clock signals is adjusted to center the data eye for the sampled half of the PRBS bits. Then all data bits (even and odd data bits) of the received PRBS bits are sampled and the phase rotator generating quadrature phase 90° and 270° clock signals is adjusted to center the data eye of all data bits of the PRBS bits to complete calibration.

Abstract: Provided herein are constructs comprising a CAR nucleic acid sequence comprising a single chain variable fragment from monoclonal antibody VAC69 for use in stimulating an immune response against solid tumors. The CAR nucleic acid sequence is linked to nucleic acids encoding one or more cytokines, and one or more of a matrix metalloprotease, a PD1 fusion protein, a chemokine receptor, a dominant negative or nonfunctional immunosuppressive or toxic receptor, a FOXP3 inhibitory peptide P60, and a Bi-specific T Cell Engager (BiTE). The constructs can further include one or more of a signal peptide, a self-cleaving peptide, an epitope tag, an internal ribosome entry site (IRES), or a selectable marker.

Abstract: In one aspect, the invention relates to methods and compositions for determining migration of a virus particle to the nucleus of a cell. This abstract is intended as a scanning tool for purposes of searching in the particular art and is not intended to be limiting of the present invention.

Abstract: Checkpoint receptors and their cognate ligands are frequently targeted in autoimmune disorders by B and T cells, wherein these adaptive immune responses are likely to significantly contribute to the underlying immunopathology. A novel technology for the clonal elimination of autoreactive B cells that targets checkpoint receptors and their ligands is described herein. One embodiment of this technology is a checkpoint receptor or ligand extracellular domain molecular chimera with an effector domain, which is capable of inducing B cell apoptosis, necrosis, and/or tolerization/anergization: herein, this technology is referred as PantIds (polyclonal anti-idiotypics). In other embodiments, this technology also includes effector molecular chimeras with immunoregulatory cytokines. Novel apoptotic effectors are also described. Methods for the identification of checkpoint receptor/ligand autoreactive B cell responses, construction of PantIds, and their in vitro and in vivo application are also described.

Abstract: In one aspect, the invention relates to methods and compositions for determining migration of a virus particle to the nucleus of a cell. This abstract is intended as a scanning tool for purposes of searching in the particular art and is not intended to be limiting of the present invention.