Abstract: The present disclosure relates in some aspects to methods and compositions for in situ detection of an analyte in a biological sample embedded in a matrix that is attached to metal nanoparticles. In some embodiments, the detection involves generation of a fluorescent signal that is enhanced by the metal nanoparticles. The fluorescent signal can be detected at a 3-dimensional (3D) location in the matrix which corresponds to the 3D location of the analyte in the biological sample.

Abstract: The present disclosure provides compositions, methods, and systems for enrichment and analysis of template nucleic acid molecules, e.g., of a biological sample. Further provided herein are methods of using a plurality of unique bead species each comprising a unique set of identical primer molecules.

Abstract: According to the invention, an Ethernet system and method of using an Ethernet system is provided including a network side device (a hub/switch/concentrator), and a plurality of end devices (either a standard end device powered locally or a system powered device), connected to the hub/switch. The system powered devices have circuitry for indicating the system power need to the hub, which has a power source for selectively supplying system power over the Ethernet. A stimulus device associated with the network device sends a stimulus signal over the network connection lines during a detection phase. The need for power is signaled by an indication device associated with the system powered end devices, providing an indication signal in response to the stimulus signal.