Abstract: Shoe outsoles having anti-infective properties are provided herein, as are methods and materials for making shoes and shoe outsoles having anti-infective properties.

Abstract: Shoe outsoles having anti-infective properties are provided herein, as are methods and materials for making shoes and shoe outsoles having anti-infective properties.

Abstract: Shoe outsoles having anti-infective properties are provided herein, as are methods and materials for making shoes and shoe outsoles having anti-infective properties.

Abstract: A novel high throughput screening (HTS) technique to rapidly identify eukaryotic topoisomerase I active agents is presented. The method is based on genetic tagging of the topoisomerase I enzyme to directly immobilize the enzyme on a solid surface in a microtiter well format. For HTS operations, DNA is added to the wells and a fraction of the input plasmid is retained on the enzyme attached to the solid phase substratum. The retained DNA is detected by Picogreen fluorescence. Compounds that result in an increase in Picogreen staining represent potential topoisomerase interfacial poisons while those that reduce fluorescence report the presence of a catalytic inhibitor; therefore, the solid phase assay represents a ‘bimodal’ readout that reveals mechanisms of action.

Abstract: Disclosed herein are novel methods for screening for compounds useful in treating telomerase positive cancers. In exemplary embodiments, screening methods are based on the implementation or manipulation of therapeutic agents that up-regulate activity or expression of MKRN polypeptides or polynucleotides encoding the same, respectively. The methods are useful in identifying agents that can serve as cancer therapeutic agents.