Abstract: Biological activity in holding pens in a micro-fluidic device can be assayed by placing in the holding pens capture objects that bind a particular material of interest produced by the biological activity. The biological material of interest that binds to each capture object can then be assessed, either in the micro-fluidic device or after exporting the capture object from the micro-fluidic device. The assessment can be utilized to characterize the biological activity in each holding pen. The biological activity can be production of the biological material of interest. Thus, the biological activity can correspond to or arise from one or more biological cells. Biological cells within a holding pen can be clonal cell colonies. The biological activity of each clonal cell colony can be assayed while maintaining the clonal status of each colony.

Abstract: A method of preparing an antibody therapeutic is provided comprising: (a) providing a dissociated cell sample from at least one solid tumor sample obtained from a patient; (b) loading the dissociated cell sample into a microfluidic device having a flow region and at least one isolation region fluidically connected to the flow region; (c) moving at least one B cell from the dissociated cell sample into at least one isolation region in the microfluidic device, thereby obtaining at least one isolated B cell; and (d) using the microfluidic device to identify at least one B cell that produces antibodies capable of binding to cancer cells. The cancer cells can be the patient's own cancer cells. Also provided are methods of treating patients, methods of labeling or detecting cancer, engineered T or NK cells comprising antibodies or fragments thereof, and engineered antibody constructs.

Abstract: The present disclosure relates generally to flexible tubing, for example, suitable for use in peristaltic pumps. The present disclosure relates more particularly to flexible tubing having a stable flow rate despite being extruded from thermoplastic polymer and methods for making such tubing. In one aspect, at least 50% of the material volume of a flexible tubing of the disclosure is formed from a first polymer material that includes a continuous phase that includes polypropylene and a non-cross-linked styrene block copolymer elastomer miscible with the polypropylene, as well as, dispersed within the continuous phase (e.g., discontinuously dispersed within the continuous phase), a cross-linked rubber phase. The non-cross-linked styrene block copolymer elastomer is present in the first polymeric material in an amount in the range of 2 wt % to 15 wt %.

Abstract: The present disclosure relates generally to flexible tubing, for example, suitable for use in peristaltic pumps. The present disclosure relates more particularly to flexible tubing having a stable flow rate despite being extruded from thermoplastic polymer and methods for making such tubing. In one aspect, at least 50% of the material volume of a flexible tubing of the disclosure is formed from a first polymer material that includes a continuous phase that includes polypropylene and a non-cross-linked styrene block copolymer elastomer miscible with the polypropylene, as well as, dispersed within the continuous phase (e.g., discontinuously dispersed within the continuous phase), a cross-linked rubber phase. The non-cross-linked styrene block copolymer elastomer is present in the first polymeric material in an amount in the range of 2 wt % to 15 wt %.