Abstract: The present disclosure is directed to systems and methods for automatically shutting off fluid flow through a fluid inlet line of a water heater upon detection of a leak of the water heater. The system may include an electrically powered shutoff assembly operatively coupled to the fluid inlet line, and a capacitor operatively coupled to the electrically powered shutoff assembly. The electrically powered shutoff assembly may be configured to shut off fluid flow through the fluid inlet line upon detection of a leak of the water heater, and the capacitor may be configured to store power received from an external power source. Accordingly, when the electrically powered shutoff assembly does not have access to electric power, the capacitor may power the electrically powered shutoff assembly to shut off fluid flow through the fluid inlet line.

Abstract: A class of integral membrane proteins, referred to as Mistic polypeptides, their variants, fusion proteins including a Mistic polypeptide domain, and nucleic acid molecules encoding Mistic polypeptides and Mistic fusion proteins are disclosed herein. Also described are methods of using Mistic polypeptides and Mistic fusion proteins to produce and/or isolate recombinant proteins (including without limitation classes of eukaryotic proteins that have previously been intractable to recombinant bacterial expression, such as, eukaryotic integral membrane proteins).