Abstract: This disclosure describes methods for detecting early stages of disease, in particular carpal tunnel syndrome, by using cooperative ultrasound techniques. In a particular embodiment, a grayscale ultrasonogram may be used to detect local anatomical features within the carpal tunnel by physically moving a tendon and identifying the corresponding feature on the ultrasonogram display device. A high-resolution color Doppler ultrasound device may then be used to interrogate features of anatomy surrounding the tendon for disease. In a particular embodiment, the color Doppler ultrasound device may be used to measure the velocities of the tendon and surrounding anatomy, in particular the sub-synovial connective tissue; the resultant data may be used to quantify certain anatomical anomalies, indicative or non-indicative of carpal tunnel syndrome.