Abstract: Current invention is directed for rapid sample pretreatment method that allows highly sensitive and specific detection of target nucleic acid (eg human genomic DNA, human pathogen genomic DNA, human non-pathogen genomic DNA) by amplification directly from crude unpurified biological samples lysates (eg human urine, saliva, blood, urethra and cervical swabs and other samples containing biological material). Invention is focused on the description of the biological sample pretreatment method that enables fast release of the genomic material from human and pathogen cells, components of what are compatible with the following nucleic acid amplification method.

Abstract: Method and rapid test device for detection of target molecule from sample with which is performed purification and preprocessing of sample, preparation and modification, amplification, detection and capturing of target molecule, producing, of signal, amplification and visualization of read 5 out signal. For purpose of invention is used a device which includes in a case 1 one or many holes 2 for injection of sample, readout 4, target molecule detection nodal points attached to case 1 which includes one or many analysis chambers 3. Analysis chamber 3 is made of at least one room space 6, dividing wall 7, chamber 8 connected with 10 room space 6 with dividing wall 9 and at least one lever 5.