Patents by Inventor Markus Aebi
Markus Aebi has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Publication number: 20220242919Abstract: Described herein are methods of producing glycosylated proteins in vitro and in vivo. The methods include using host cells to produce glycosylated proteins. Also described herein are glycosylated proteins produced using such methods and uses thereof.Type: ApplicationFiled: December 17, 2021Publication date: August 4, 2022Inventors: Michael L. Wetter, Michael T. Kowarik, Amirreza Faridmoayer, Manuela Mally, Christian A. Lizak, Markus Aebi, Chia-wei Lin, Ivan Hang, Timothy Keys
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Patent number: 11236136Abstract: Described herein are methods of producing glycosylated proteins in vitro and in vivo. The methods include using host cells to produce glycosylated proteins. Also described herein are glycosylated proteins produced using such methods and uses thereof.Type: GrantFiled: November 30, 2016Date of Patent: February 1, 2022Assignees: LIMMATECH BIOLOGICS AG, EIDGENÖSSISCHE TECHNISCHE HOCHSCHULE ZÜRICHInventors: Michael L. Wetter, Michael T. Kowarik, Amirreza Faridmoayer, Manuela Mally, Christian A. Lizak, Markus Aebi, Chia-wei Lin, Ivan Hang, Timothy Keys
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Patent number: 11180738Abstract: The present invention is directed to all aspects of novel methyl transferase enzymes that methylate backbone amides of (poly)peptides. The present invention also relates to nucleic acids encoding these enzymes as well as corresponding vectors and host cells comprising these. Moreover, the present invention encompasses the use of said enzymes for modifying (poly)peptides as well as corresponding methods. Also, the present invention pertains to further novel enzymes for modifying (poly)peptides derived from the omphalotin gene cluster of O. olearius and the homologous gene clusters from D. bispora, L. edodes and F. mediterranea as well as related aspects.Type: GrantFiled: April 7, 2017Date of Patent: November 23, 2021Assignee: ETH ZURICHInventors: Markus Aebi, Markus Kunzler, Jorn Piel, Michael Freeman, Niels Van Der Velden, Noemi Kalin
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Publication number: 20190112583Abstract: The present invention is directed to all aspects of novel methyl transferase enzymes that methylate backbone amides of (poly)peptides. The present invention also relates to nucleic acids encoding these enzymes as well as corresponding vectors and host cells comprising these. Moreover, the present invention encompasses the use of said enzymes for modifying (poly)peptides as well as corresponding methods. Also, the present invention pertains to further novel enzymes for modifying (poly)peptides derived from the omphalotin gene cluster of O. olearius and the homologous gene clusters from D. bispora, L. edodes and F. mediterranea as well as related aspects.Type: ApplicationFiled: April 7, 2017Publication date: April 18, 2019Inventors: Markus AEBI, Markus KUNZLER, Jorn PIEL, Michael FREEMAN, Niels VAN DER VELDEN, Noemi KALIN
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Publication number: 20180354997Abstract: Described herein are methods of producing glycosylated proteins in vitro and in vivo. The methods include using host cells to produce glycosylated proteins. Also described herein are glycosylated proteins produced using such methods and uses thereof.Type: ApplicationFiled: November 30, 2016Publication date: December 13, 2018Inventors: Michael L. Wetter, Michael T. Kowarik, Amirreza Faridmoayer, Manuela Mally, Christian A. Lizak, Markus Aebi, Chia-wei Lin, Ivan Hang, Timothy Keys
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Patent number: 9881124Abstract: The present invention is directed to methods for identifying or designing (a) a potential oligosaccharide donor, (b) a potential oligosaccharyltransferase (OST), (c) a potential consensus sequence motif polypeptide, and/or (d) a potential glycosylation inhibitor for use in the oligosaccharyltransferase (OST)—catalysed asparagine-linked (“N-linked”) glycosylation, comprising the steps of generating a three-dimensional model of the catalytic domain and/or the polypeptide binding site of the oligosaccharyltransferase (OST) of Campylobacter lari, and designing or selecting a potential component selected from (a) to (d) which optimizes the stereo chemical complementarity of said three-dimensional model(s) and the potential component.Type: GrantFiled: May 3, 2012Date of Patent: January 30, 2018Assignee: ETH ZURICHInventors: Markus Aebi, Kaspar Locher, Christian Lizak
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Patent number: 9764021Abstract: The present invention relates to Salmonella enterica comprising at least pgl operon of Campylobacter jejuni or a functional derivative thereof and presenting at least one N-glycan of Campylobacter jejuni or N-glycan derivative thereof on its cell surface and, in particular, to medical uses and pharmaceutical compositions thereof as well as methods for treating and/or preventing Campylobacter and optionally Salmonella infections and methods for producing these Salmonella strains.Type: GrantFiled: April 8, 2016Date of Patent: September 19, 2017Assignee: EIDGENOESSISCHE TECHNISCHE HOCHSCHULE ZURICHInventors: Karin Ilg, Markus Aebi, Umesh Ahuja, Saba Amber, Flavio Schwartz
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Patent number: 9551019Abstract: The present invention relates to recombinant N-glycosylated proteins, comprising one or more introduced N-glycosylated optimized amino acid sequence(s), nucleic acids encoding these proteins as well as corresponding vectors and host cells. In addition, the present invention is directed to the use of said proteins, nucleic acids, vectors and host cells for preparing medicaments. Furthermore, the present invention provides methods for producing said proteins.Type: GrantFiled: February 6, 2014Date of Patent: January 24, 2017Assignee: ETH ZÜRICHInventors: Markus Aebi, Michael Kowarik, Umesh Ahuja
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Publication number: 20160326563Abstract: The present invention relates to recombinant N-glycosylated proteins, comprising one or more introduced N-glycosylated optimized amino acid sequence(s), nucleic acids encoding these proteins as well as corresponding vectors and host cells. In addition, the present invention is directed to the use of said proteins, nucleic acids, vectors and host cells for preparing medicaments. Furthermore, the present invention provides methods for producing said proteins.Type: ApplicationFiled: May 19, 2016Publication date: November 10, 2016Applicant: ETH ZÜRICHInventors: Markus Aebi, Michael Kowarik, Umesh Ahuja
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Publication number: 20160213770Abstract: The present invention relates to Salmonella enterica comprising at least pgl operon of Campylobacter jejuni or a functional derivative thereof and presenting at least one N-glycan of Campylobacter jejuni or N-glycan derivative thereof on its cell surface and, in particular, to medical uses and pharmaceutical compositions thereof as well as methods for treating and/or preventing Campylobacter and optionally Salmonella infections and methods for producing these Salmonella strains.Type: ApplicationFiled: April 8, 2016Publication date: July 28, 2016Inventors: Karin Ilg, Markus Aebi, Umesh Ahuja, Saba Amber, Flavio Schwartz
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Patent number: 9309493Abstract: The present invention relates to Salmonella enterica comprising at least one pgl operon of Campylobacter jejuni or a functional derivative thereof and presenting at least one N-glycan of Campylobacter jejuni or N-glycan derivative thereof on its cell surface. In addition, it is directed to medical uses and pharmaceutical compositions thereof as well as methods for treating and/or preventing Campylobacter and optionally Salmonella infections and methods for producing these Salmonella strains.Type: GrantFiled: March 25, 2010Date of Patent: April 12, 2016Assignee: Eidgenoessische Technische Hochschule ZurichInventors: Karin Ilg, Markus Aebi, Umesh Ahuja, Saba Amber, Flavio Schwarz
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Publication number: 20150203828Abstract: The present invention relates to new galactosyltransferases, nucleic acids encoding them, as well as recombinant vectors, host cells, antibodies, uses and methods relating thereto.Type: ApplicationFiled: February 21, 2014Publication date: July 23, 2015Applicants: ETH ZURICH, UNIVERSITAT FUR BODENKULTUR WIEN, UNIVERSITAT ZURICHInventors: Markus KÜNZLER, Markus AEBI, Iain WILSON, Alexander Walter TITZ, Micahel HENGARTNER, Alex BUTSCHI
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Publication number: 20140335127Abstract: A system and a method for the production of recombinant N-glycosylated target proteins. The system comprises a prokaryotic organism (e.g. Escherichia coli) into which is introduced a genetic information encoding for a metabolic apparatus capable of carrying out the requested N-glycosylation of the target protein. Said prokaryotic organism also contains the genetic information required for the expression of one or more recombinant target proteins. The metabolic apparatus preferably comprises specific glycosyltransferases for the assembly of the oligosaccharide on a lipid carrier and an OTase that covalently links this oligosaccharide to specific residues of the desired protein.Type: ApplicationFiled: February 25, 2014Publication date: November 13, 2014Applicant: ETH ZÜRICHInventors: Markus Aebi, Michael Wacker
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Publication number: 20140323700Abstract: The present invention relates to recombinant N-glycosylated proteins, comprising one or more introduced N-glycosylated optimized amino acid sequence(s), nucleic acids encoding these proteins as well as corresponding vectors and host cells. In addition, the present invention is directed to the use of said proteins, nucleic acids, vectors and host cells for preparing medicaments. Furthermore, the present invention provides methods for producing said proteins.Type: ApplicationFiled: February 6, 2014Publication date: October 30, 2014Applicant: ETH ZÜRICHInventors: Markus Aebi, Michael Kowarik, Umesh Ahuja
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Publication number: 20140194346Abstract: The present invention relates to an N-glycosylated protein for treating and/or preventing bacterial Pasteurellaceae infection in a mammal or bird, wherein the protein is a Pasteurellaceae protein, a functional fragment or derivative thereof having at least one glycosylated N-X-S/T consensus sequence. In addition, the present invention is directed to corresponding pharmaceutical compositions for treating and/or protecting mammals or birds having or being prone to develop a bacterial Pasteurellaceae infection. Furthermore, the invention describes methods for producing said N-glycosylated proteins.Type: ApplicationFiled: August 7, 2012Publication date: July 10, 2014Applicant: ETH ZURICHInventors: Markus Aebi, Flavio Schwarz, Andreas Naegeli
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Patent number: 8753864Abstract: The present invention relates to recombinant N-glycosylated proteins, comprising one or more introduced N-glycosylated optimized amino acid sequence(s), nucleic acids encoding these proteins as well as corresponding vectors and host cells. In addition, the present invention is directed to the use of said proteins, nucleic acids, vectors and host cells for preparing medicaments. Furthermore, the present invention provides methods for producing said proteins.Type: GrantFiled: May 10, 2006Date of Patent: June 17, 2014Assignee: ETH ZurichInventors: Markus Aebi, Michael Kowarik, Umesh Ahuja
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Patent number: 8703471Abstract: A system and a method for the production of recombinant N-glycosylated target proteins. The system comprises a prokaryotic organism (e.g. Escherichia coli) into which is introduced a genetic information encoding for a metabolic apparatus capable of carrying out the requested N-glycosylation of the target protein. Said prokaryotic organism also contains the genetic information required for the expression of one or more recombinant target proteins. The metabolic apparatus preferably comprises specific glycosyltransferases for the assembly of the oligosaccharide on a lipid carrier and an OTase that covalently links this oligosaccharide to specific residues of the desired protein.Type: GrantFiled: July 21, 2008Date of Patent: April 22, 2014Assignee: ETH ZürichInventors: Markus Aebi, Michael Wacker
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Publication number: 20140100836Abstract: The present invention is directed to methods for identifying or designing (a) a potential oligosaccharide donor, (b) a potential oligosaccharyltransferase (OST), (c) a potential consensus sequence motif polypeptide, and/or (d) a potential glycosylation inhibitor for use in the oligosaccharyltransferase (OST)-catalysed asparagine-linked (“N-linked”) glycosylation, comprising the steps of generating a three-dimensional model of the catalytic domain and/or the polypeptide binding site of the oligosaccharyltransferase (OST) of Campylobacter lari, and designing or selecting a potential component selected from (a) to (d) which optimizes the stereo chemical complementarity of said three-dimensional model(s) and the potential component.Type: ApplicationFiled: May 3, 2012Publication date: April 10, 2014Applicant: ETH ZURICHInventors: Markus Aebi, Kaspar Locher, Christian Lizak
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Publication number: 20130040897Abstract: The invention provides means and methods for an improved production of glycosylated recombinant proteins in lower eukaryotes, specifically the production of human-like complex or hybrid glycosylated proteins in yeast. The invention provides genetically modified eukaryotic host cells capable of producing glycosylation optimized proteins useful as immunoglobulins and other therapeutic proteins, and provides cells capable of producing glycoproteins having glycan structures similar to glycoproteins produced in human cell. The invention further provides proteins with human-like glycan structures and novel compositions thereof producible by these modified cells.Type: ApplicationFiled: April 27, 2011Publication date: February 14, 2013Applicant: LONZA LTD.Inventors: Markus Aebi, Farnoush Parsaie Nasab, Alexander Daniel Frey
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Publication number: 20120207737Abstract: The present invention relates to the use of glycan-binding polypeptides and glycans as a medicament, in particular for treating and/or preventing helminthic infections or an immune disease. Moreover, the present invention is directed to corresponding pharmaceutical compositions, food products and animal feed comprising isolated glycans and/or glycan-binding polypeptides. In addition, the present invention teaches methods for identifying anti-helminthic carbohydrate-binding polypeptides, for identifying helminthic glycan and gene targets involved in glycan-mediated toxicity, for identifying helminths susceptible to glycan-mediated toxicity, and for identifying anti-helminthic and anti-allergic substances.Type: ApplicationFiled: October 12, 2010Publication date: August 16, 2012Applicants: UNIVERSITY OF ZURICH, ETH ZURICHInventors: Markus Kunzler, Martin Walti, Alex Butschi, Markus Aebi, Michael Hengartner