Abstract: An optical assembly for the illumination and hyperspectral evaluation of an object, having a light source or an optical element at which a light source radiates, wherein the light source or the optical element is designed to divide pairs of unambiguously assignable photons into a first light beam and a second light beam so that the first light beam hits a first detector system and the second light beam is directed at an object and light radiation coming from the object is directed at an optical element which spectrally decomposes light radiation and, from the optical element spectrally decomposing said light radiation, is directed at a second detector system. The first light beam can also be directed at a spectrally decomposing optical element and, from there, at a first detector system, and the light radiation coming from the object can be directed directly at the second detector system.

Abstract: In the optical arrangement for fluorescent microscopic applications, one or more multiphoton beams, but at least one or two photon pair beams, from a source of non-classical light is/are directed at a first optical system, consisting of an arrangement of at least one lens or one photon-reflecting element or another beam-forming element or a combination thereof. The first optical system (3) is designed to shape the non-classical light into a light sheet (4) or a light sheet-like shape and thence to direct it at a specimen (5), so that fluorescent radiation is excited by means of multiphoton absorption using the multiple multiphoton beams that are simultaneously incident on/in the specimen. Fluorescent radiation (6) obtained by excitation is incident by means of a second optical system (7) on a detection system (8) that is designed for the spatially resolved capture of fluorescent radiation.

Abstract: An optical arrangement for fluorescence microscopy applications. Electromagnetic radiation from a radiation source is directed onto a biological sample in the form of a light sheet. One of more fluorophore(s) is contained in the sample. The radiation photoactivates the fluorophore(s) by exciting them from a state which they cannot be exited to fluoresce to a state which they can be exited to fluoresce by illuminating with electromagnetic radiation of a particular wavelength, and subsequently photodeactivating them. Multiphoton beams of nonclassical light are directed onto a first optical system the beam(s) are directed onto a sample of the light sheet. Fluorescent radiation of fluorophores, can be excited within the light sheet by the plurality of multiphoton beams occurring simultaneously on/in the sample. The fluorescence radiation occurs by means of a second optical system on a detection system which measures in a spatially resolving manner.

Abstract: The invention relates to the optical assembly for the illumination and hyperspectral evaluation of an object, having a light source or an optical element (1) at which a light source radiates, wherein the light source or the optical element is designed to divide pairs of unambiguously assignable photons into a first light beam (2) and a second light beam (5) in such a way that the first light beam hits a first detector system (4) and the second light beam is directed at an object (7) and light radiation coming from the object is directed at an optical element (8) which spectrally decomposes said light radiation and, from the optical element spectrally decomposing said light radiation is directed at a second detector system (9). The first light beam can also be directed at a spectrally decomposing optical element and from there, at a first detector system, and the light radiation coming from the object can be directed directly at the second detector system.