Abstract: A method for controlling microscopic imaging of a microscope includes providing a microscope control arrangement configured for receiving a focusing request and for receiving sample information on a sample to be imaged, wherein the microscope control arrangement activates, upon receipt of a focusing request and after having received the sample information, a predefined focusing setting depending on the sample information received for controlling focusing of the microscope for microscopic imaging of the sample.

Abstract: The first aspect of this disclosure is related to a computer-based method for measurement of a plurality of optical foci, comprising the steps: measuring a first focus; obtaining one or both of: a) a focus range for a measurement of a second focus, wherein the focus range is based on the first focus; b) a starting value for a measurement of a second focus, wherein the starting value is based on the first focus; measuring the second focus with the obtained focus range and/or staring value; discarding the second focus, if the second focus is measured as an extreme value of the focus range; measuring a third focus.

Abstract: A first aspect of this disclosure relates to a computer-based method of setting an optical focus, comprising the steps of: obtaining a first threshold value for a drift of an optical focus, obtaining a first drift value, wherein it is based on measurements of at least one parameter regarding the focus at different points in time, comparing the first drift value with the first threshold value, outputting the first drift value if it exceeds the first threshold value inadmissibly.

Abstract: The invention relates to a data processing device for an imaging apparatus, the device being configured to obtain a length threshold value, to access input image data representing at least one digital input image, wherein the input image data comprise a shading signal representing a brightness decrease towards the edges of the at least one digital input image, and a content signal representing image features of the at least one digital input image, the image features having a length that is smaller than the length threshold value, to compute a baseline image based on the input image data and the length threshold value, wherein the baseline image is representative of an estimate of the shading signal, to generate at least one digital output image representative of an estimate of the content signal by at least one of a) subtracting the baseline image from the input image data and b) dividing the input image data by the baseline image.

Abstract: An image processing apparatus for determining a focused output image in a passive autofocus system is configured to retrieve a set of input images and compute a baseline estimate for at least one input image. The baseline estimate represents image structures in the input image. The image structures have a length scale larger than a predetermined image feature length scale. The image processing apparatus is further configured to compute a set of output images, wherein each output image of the set of output images is computed based on one of a different input image of the set of input images and the at least one baseline estimate for the different input image and the at least one baseline estimate for a respective different input image. The image processing apparatus is further configured to determine one output image of the set of output images as the focused output image.

Abstract: An apparatus for a microscope system is provided. The apparatus comprising one or more processors and one or more storage devices. The one or more processors are configured to receive region information about an accessible spatial region for an objective of the microscope system and to trigger a restriction of a relative movement of the objective based on the region information.

Abstract: A microscope control arrangement for operating a focusing device adapted to adjust a relative focus position of an objective in relation to a sample along an optical axis, including a display device configured to display a graphical user interface for receiving a user input and at least one processor configured to control the microscope focusing device and the display device for updating the graphical user interface in response to the user input, wherein the processor is configured to cause the graphical user interface to display a focus control interface including a graphical focus range element indicating a predetermined focus range and a graphical z-position pointer indicating a current z-position within the predetermined focus range, and wherein the graphical z-position pointer is movable along the graphical focus range element responsive to the user input to adjust the relative focus position to the current z-position indicated by the graphical z-position pointer.

Abstract: An image processing apparatus for determining a focused output image in a passive autofocus system is configured to retrieve a set of input images and compute a baseline estimate for at least one input image. The baseline estimate represents image structures in the input image. The image structures have a length scale larger than a predetermined image feature length scale. The image processing apparatus is further configured to compute a set of output images, wherein each output image of the set of output images is computed based on one of a different input image of the set of input images and the at least one baseline estimate for the different input image and the at least one baseline estimate for a respective different input image. The image processing apparatus is further configured to determine one output image of the set of output images as the focused output image.