Abstract: A normothermic machine perfusion method for a pancreas is provided. First, a perfusate contained in a venous reservoir may be oxygenated such that a ratio of oxygen to carbon dioxide in the oxygenated perfusate is approximately 90%/10%. A first portion of the oxygenated perfusate may be pumped from the venous reservoir, through a dialysis filter, and back to the venous reservoir. A dialysate comprising a salt and glucose may be infused into the first portion of the oxygenated perfusate as the first portion is pumped through the dialysis filter. A concentration of glucose in the dialysate may be less than 8 mmol/L. A second portion of the oxygenated perfusate may be pumped through an arterial filter and through a pancreas graft. Venous outflow from the pancreas graft may then be pumped to the venous reservoir.

Abstract: The present disclosure provides a method of treating or preventing hepatic ischemia and reperfusion injury in an animal comprising administering an effective amount of an FGL2-Fc?RIIB inhibitor to an animal in need thereof. Also provided herein are uses, compositions and screening assays for FGL2- Fc?RIIB inhibitors useful in treating hepatic ischemia and reperfusion injury.