Abstract: The invention is directed to methods for producing somatic embryos of Rosa hybrida and obtaining regenerated plantlets therefrom. Somatic embryos may be produced by culturing mature tissue from Rosa hybrida in callus induction medium to induce callus formation and subsequently culturing the induced callus in regeneration medium which is capable of inducing completion of the development of somatic embryos. The availability of somatic embryos and the regenerative capacity of such embryos provides convenient raw material for carrying out current methods of plant transformation, such as ballistic methods of DNA delivery, or Agrobacterium culture.

Abstract: This invention relates to somaclonal variants from different varieties of coffee plants. The invention also contemplates methods for the high frequency generation of and the low frequency generation of said somaclonal variants in coffee plants, thereby providing variants with improved processing and agronomic characteristics. Among these somaclonal variants are high-yielding, low caffeine Coffea arabica cv. Laurina somaclones and a method for producing same. In particular, this invention relates to the unique application of tissue culture methodology as new methods for variety development, breeding and then the scale-up of the selected superior genotypes of coffee plants for the commercial production of coffee.

Abstract: The present invention provides a method for regeneration of somatic embryos from non-zygotic or zygotic tissue. The invention also provides a means for regeneration of cacao plantlets and plants from somatic embryos.

Abstract: The present invention relates to novel sweet or supersweet corn hybrids with improved yield and germination characteristics. The hybrids of the present invention are produced by crossing flint and dent field corn parents.

Abstract: This invention provides a method for high frequency plant regeneration from somatic stem donor tissue of field grown Zea diploperennis, a diploid, perennial corn ancestor with high tillering capacity. This species is used as a parent in a maize improvement strategy to transfer the unique traits of high tillering and plantlet regeneration capacity into cultivated corn. After 3-4 subcultures of cultured somatic tissues on a primary medium, small callus fragments are transferred to a secondary medium devoid of the auxin, 2,4-D. After a few days, numerous shoots regenerate and develop into normal plantlets which are then separated and transferred to a tertiary medium for root development. The selection of somaclonal variants form cultured somatic cells of interspecific hybrids between corn and teosinte are used for the synthesis of unique breeding lines suited for development of improved corn varieties. A protocol for gene transfer employing recombinant DNA techniques is also described.

Abstract: This invention provides a method for high frequency plant regeneration from somatic stem donor tissue of field grown Zea diploperennis, a diploid, perennial corn ancestor with high tillering capacity. This species is used as a parent in a maize improvement strategy to transfer the unique traits of high tillering and plantlet regeneration capacity into cultivated corn. After 3-4 subcultures of cultured somatic tissues on a primary medium, small callus fragments are transferred to secondary medium devoid of the auxin, 2,4-D. After a few days, numerous shoots regenerate and develop into normal plantlets which are then separated and transferred to a tertiary medium for root development. The selection of somaclonal variants form cultured somatic cells of interspecific hybrids between corn and teosinte are used for the synthesis of unique breeding lines suited for development of improved corn varieties. A protocol for gene transfer employing recombinant DNA techniques is also described.