Abstract: The invention relates to an immunogenic or vaccine composition comprising an inactivated bacterium from the species Brachyspira hyodysenteriae, to a method for producing said composition and to the medical use of this composition for inducing an immune response against a bacterium from the species Brachyspira hyodysenteriae or for protecting against an infection caused by a bacterium from the species Brachyspira hyodysenteriae. Further, the invention relates to a method for selecting a bacterium from the species Brachyspira hyodysenteriae useful for manufacturing a vaccine against swine dysentery.

Abstract: Provided are compounds of Formula (I?): or pharmaceutically acceptable salts thereof, wherein the variables in the formula are as defined herein; and methods for their use and production.

Abstract: Methods of decreasing bead aggregation and improving specificity of nucleic acid hybridization using non-specific nucleic acid binding proteins is described.

Abstract: Methods of generating cDNAs from RNA involving cleavage of DNA/RNA complexes are provided.

Abstract: A computing device comprising a processor is disclosed herein. The processor is to access print data of a virtual build volume including a plurality of 3D objects to be generated through a selective application of a binder agent by a 3D printer. The processor is further to modify the print data to include a 3D structure at a location within the build volume to protect a 3D object from migrating solvents of the binder agent during a curing operation.

Abstract: A build platform of an additive manufacturing apparatus is disclosed. The build platform includes a surface formed of a material having a first emissivity; and a plurality of reference markers disposed on the surface. The reference markers have a second emissivity that is different from the first emissivity. A thermal imaging unit calibration method and additive manufacturing apparatus are also disclosed.

Abstract: A sensor calibration method is disclosed. The method includes applying thermal energy to a fabrication chamber of an additive manufacturing apparatus to raise a temperature of a surface within the fabrication chamber to a first surface temperature; measuring, using a first sensor to be calibrated, the first surface temperature; measuring, using a second sensor, the first surface temperature; applying thermal energy to the fabrication chamber to raise a temperature of the surface to a second surface temperature; measuring, using the first sensor, the second surface temperature; measuring, using the second sensor, the second surface temperature; determining, using a processor, based on the first and second surface temperatures measured using the first sensor and on the first and second surface temperatures measured using the second sensor, an offset calibration to be applied to measurements obtained using the first sensor; and applying the offset calibration to measurements obtained using the first sensor.

Abstract: Methods of coupling adaptors to a target nucleic acid include coupling a first adaptor to a first end of the target nucleic acid to form a coupled first adaptor. A portion of a second adaptor is hybridized to a portion of the coupled first adaptor to form a hybridized second adaptor having a single-stranded 3?-end. The hybridized second adaptor is coupled to a second end of the target nucleic acid to form an adaptor-flanked product having at least a part of the first adaptor coupled to the first end of the target nucleic acid and at least a part of the second adaptor coupled to the second end of the target nucleic acid. These methods can minimize the formation of adaptor-dimers that may be problematic in subsequent complementary nucleic acid strand synthesis, amplification, and sequencing.

Abstract: Provided are methods for generating strand specific nucleic acids. The subject methods may include coupling an adaptor to a 3?-end of a target nucleic acid to form an adaptor-coupled target nucleic acid, which may be combined with further components in a template switching reaction to produce a product nucleic acid. The subject methods find use in a variety of applications, including but not limited to e.g., the preparation of nucleic acid libraries.

Abstract: Provided herein are compositions for and methods of generating ligation-competent nucleic acids. In some aspects, the compositions comprise Exonuclease III, T4 DNA Polymerase, Klenow, and/or T4 polynucleotide kinase.

Abstract: A positioning device for a medical field generator includes a base member, an adaptor connected with the base member and configured to support a field generator, and a fixing member allowing the positioning device to be fixed to an associated operating table. The base member includes at least one contacting section and is configured to contact an upper surface of an operating table. The adaptor is configured to prevent the field generator from being positioned at less than a predetermined distance from the operating table. A method positions a medical field generator. The field generator is attached with the positioning device. A base member of the positioning device, with the field generator attached to it, is positioned on the upper surface of the operating table and beneath a part of a head of a patient lying on the operating table. The positioning device is fixedly attached with the operating table.

Abstract: Methods of coupling adaptors to a target nucleic acid include coupling a first adaptor to a first end of the target nucleic acid to form a coupled first adaptor. A portion of a second adaptor is hybridized to a portion of the coupled first adaptor to form a hybridized second adaptor having a single-stranded 3?-end. The hybridized second adaptor is coupled to a second end of the target nucleic acid to form an adaptor-flanked product having at least a part of the first adaptor coupled to the first end of the target nucleic acid and at least a part of the second adaptor coupled to the second end of the target nucleic acid. These methods can minimize the formation of adaptor-dimers that may be problematic in subsequent complementary nucleic acid strand synthesis, amplification, and sequencing.

Abstract: Provided herein are compositions for and methods of generating ligation-competent nucleic acids. In some aspects, the compositions comprise Exonuclease III, T4 DNA Polymerase, Klenow, and/or T4 polynucleotide kinase.

Abstract: The present invention relates to a positioning device (1) for a medical field generator (2), comprising:—a base member (3) which comprises at least one contacting section (3a) and is configured to contact the upper surface of an operating table;—an adaptor (4) which is connected to the base member (3) and configured to support the field generator (2); and—fixing means (5) which allow the positioning device (1) to be fixed to an operating table, wherein the adaptor (4) is configured to prevent the field generator (2) from being positioned at less than a predetermined distance from the operating table.

Abstract: A surgical thread includes an elongate, preferably braided, thread body and a coating surrounding the thread body at least partially and preferably completely, wherein the thread body includes polyethylene and/or polypropylene and the coating consists of a resorbable material and optionally additives.

Abstract: An apparatus and method for providing a retractable self-leveling ceiling sign hanger. The apparatus comprises of a sign housing having an upper housing and a lower housing, a cord reel housing, and a cord that forms a continuous loop between the sign housing and the cord reel housing. The sign housing and the cord reel housing coact to transfer or extend cord between them to thereby raise and lower the lower housing in a level position for installing, changing, and hanging a sign. The sign housing utilizes roller assemblies and a cord lock mechanism. The cord reel housing utilizes a handle, a tool, a locking means, and a cord retracting means for moving the cord reel housing and transferring or extending cord to and from the cord reel housing. Magnets are also provided to affix the cord reel housing to the upper housing of the ceiling sign hanger.

Abstract: A platform bed assembly, to be used by a child as a sleeping area and a play area, the bed assembly includes a lower base structure structured and disposed to resemble a large scale version of a toy article such as a van or house. The platform bed assembly includes a mattress support platform positioned and secured atop the base structure whereon a mattress may be placed in overlying position, thereby defining a sunken sleeping area, includes a ladder secured to the base structure so as to allow faciliated access to the sleeping area, and includes a door in a side wall of the base structure to allow access to an interior compartment defining the play area. Additionally, there are a plurality of toy accessory components positioned within the interior compartment which correspond the design of the toy article, and add realism to the play environment.

Abstract: A collapsible doghouse, to be used indoors or outdoors, to provide a comfortable and easy to clean place for a dog to rest or seek shade. The doghouse includes elongate side walls, a pair of distal walls, one of which includes an arched entranceway and another of which includes a cutout drainage slot, a roof portion, and a weightable base portion, all of which are securely, yet easily removably connected, and are formed of a substantially solid, yet flexible, water repellant plastic which will facilitate easy cleaning and drainage, and lightweight transportation.