Patents by Inventor Martha A. O'Brien
Martha A. O'Brien has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Patent number: 9951372Abstract: The present invention provides compounds and methods for assaying redox state of metabolically active cells and methods for assaying enzyme activity and/or metabolite level by coupling to redox defining co-factor NAD(P)/NAD(P)H measurement.Type: GrantFiled: September 20, 2013Date of Patent: April 24, 2018Assignee: PROMEGA CORPORATIONInventors: Wenhui Zhou, Jolanta Vidugiriene, Helene A. Benink, James J. Cali, Sarah Duellman, Dieter Klaubert, Donna Leippe, Martha O'Brien, John Shultz, Mary Sobol
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Patent number: 9631225Abstract: A method and kit are provided for enhancing the tolerance of an assay reagent to compounds in an assay sample, the assay reagent including a luciferase enzyme. The method includes contacting the luciferase with a tolerance enhancement agent in an amount sufficient to substantially protect luciferase enzyme activity from interference of the compound and minimize interference by at least about 10% relative to an assay not having tolerance enhancement agent.Type: GrantFiled: October 14, 2014Date of Patent: April 25, 2017Assignee: PROMEGA CORPORATIONInventors: Erika Hawkins, James J. Cali, Samuel Kin Sang Ho, Martha A. O'Brien, Richard Somberg, Robert F. Bulleit, Keith V. Wood
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Patent number: 9273343Abstract: The present invention provides compounds and methods for assaying redox state of metabolically active cells and methods for assaying enzyme activity and/or metabolite level by coupling to redox defining co-factor NAD(P)/NAD(P)H measurement.Type: GrantFiled: August 31, 2012Date of Patent: March 1, 2016Assignee: PROMEGA CORPORATIONInventors: James J. Cali, Sarah Duellman, Dieter Klaubert, Donna Leippe, Martha O'Brien, John Shultz, Jolanta Vidugiriene, Wenhui Zhou, Mary Sobol
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Publication number: 20150337359Abstract: A method and kit are provided for enhancing the tolerance of an assay reagent to compounds in an assay sample, the assay reagent including a luciferase enzyme. The method includes contacting the luciferase with a tolerance enhancement agent in an amount sufficient to substantially protect luciferase enzyme activity from interference of the compound and minimize interference by at least about 10% relative to an assay not having tolerance enhancement agent.Type: ApplicationFiled: October 14, 2014Publication date: November 26, 2015Inventors: Erika Hawkins, James J. Cali, Samuel Kin Sang Ho, Martha A. O'Brien, Richard Somberg, Robert F. Bulleit, Keith V. Wood
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Patent number: 8962266Abstract: The present invention provides compounds and methods for assaying activities of enzymes such as histone deacetylases and histone acetyltransferases. In some embodiments, the methods may be performed in one step. The compounds described herein features peptide-based compounds having at least one blocked lysine or arginine residue which are coupled to reporter moieties. The methods described herein involve reacting a compound described herein with an enzyme, such as a histone deacetylase enzyme or a histone acetyltransferase enzyme, and an endopeptidase that recognizes basic amino acids to release the reporter moiety which may be subsequently detected.Type: GrantFiled: November 26, 2013Date of Patent: February 24, 2015Assignee: Promega CorporationInventors: Thomas Kirkland, Andrew L. Niles, Martha O'Brien, Carolyn Woodroofe Hitko
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Patent number: 8859220Abstract: A method and kit are provided for enhancing the tolerance of an assay reagent to compounds in an assay sample, the assay reagent including a luciferase enzyme. The method includes contacting the luciferase with a tolerance enhancement agent in an amount sufficient to substantially protect luciferase enzyme activity from interference of the compound and minimize interference by at least about 10% relative to an assay not having tolerance enhancement agent.Type: GrantFiled: January 25, 2013Date of Patent: October 14, 2014Assignee: Promega CorporationInventors: Erika Hawkins, James J. Cali, Samuel Kin Sang Ho, Martha A. O'Brien, Richard Somberg, Robert F. Bulleit, Keith V. Wood
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Publication number: 20140099655Abstract: The present invention provides compounds and methods for assaying activities of enzymes such as histone deacetylases and histone acetyltransferases. In some embodiments, the methods may be performed in one step. The compounds described herein features peptide-based compounds having at least one blocked lysine or arginine residue which are coupled to reporter moieties. The methods described herein involve reacting a compound described herein with an enzyme, such as a histone deacetylase enzyme or a histone acetyltransferase enzyme, and an endopeptidase that recognizes basic amino acids to release the reporter moiety which may be subsequently detected.Type: ApplicationFiled: November 26, 2013Publication date: April 10, 2014Applicant: Promega CorporationInventors: Thomas Kirkland, Andrew L. Niles, Martha O'Brien, Carolyn Woodroofe Hitko
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Publication number: 20140093894Abstract: The present invention provides compounds and methods for assaying redox state of metabolically active cells and methods for assaying enzyme activity and/or metabolite level by coupling to redox defining co-factor NAD(P)/NAD(P)H measurement.Type: ApplicationFiled: September 20, 2013Publication date: April 3, 2014Applicant: PROMEGA CORPORATIONInventors: Helene A. BENINK, James J. CALI, Sarah DUELLMAN, Dieter KLAUBERT, Donna LEIPPE, Martha O'BRIEN, John SHULTZ, Jolanta VIDUGIRIENE, Wenhui ZHOU, Mary SOBOL
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Patent number: 8603767Abstract: A method and kit are provided for enhancing the tolerance of an assay reagent to compounds in an assay sample, the assay reagent including a luciferase enzyme. The method includes contacting the luciferase with a tolerance enhancement agent in an amount sufficient to substantially protect luciferase enzyme activity from interference of the compound and minimize interference by at least about 10% relative to an assay not having tolerance enhancement agent.Type: GrantFiled: January 25, 2013Date of Patent: December 10, 2013Assignee: Promega CorporationInventors: Erika Hawkins, James J. Cali, Samuel Kin Sang Ho, Martha A. O'Brien, Richard Somberg, Robert F. Bulleit, Keith V. Wood
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Publication number: 20130130289Abstract: The present invention provides compounds and methods for assaying redox state of metabolically active cells and methods for assaying enzyme activity and/or metabolite level by coupling to redox defining co-factor NAD(P)/NAD(P)H measurement.Type: ApplicationFiled: August 31, 2012Publication date: May 23, 2013Applicant: PROMEGA CORPORATIONInventors: Helene A. BENINK, James J. CALI, Sarah DUELLMAN, Dieter KLAUBERT, Donna LEIPPE, Martha O'BRIEN, John SHULTZ, Jolanta VIDUGIRIENE, Wenhui ZHOU, Mary SOBOL
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Patent number: 8361739Abstract: A method and kit are provided for enhancing the tolerance of an assay reagent to compounds in an assay sample, the assay reagent including a luciferase enzyme. The method includes contacting the luciferase with a tolerance enhancement agent in an amount sufficient to substantially protect luciferase enzyme activity from interference of the compound and minimize interference by at least about 10% relative to an assay not having tolerance enhancement agent.Type: GrantFiled: June 21, 2010Date of Patent: January 29, 2013Assignee: Promega CorporationInventors: Erika Hawkins, James J. Cali, Samuel Kin Sang Ho, Martha A. O'Brien, Richard Somberg, Robert F. Bulleit, Keith V. Wood
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Publication number: 20120064554Abstract: The present invention provides compounds and methods for assaying activities of enzymes such as histone deacetylases and histone acetyltransferases. In some embodiments, the methods may be performed in one step. The compounds described herein features peptide-based compounds having at least one blocked lysine or arginine residue which are coupled to reporter moieties. The methods described herein involve reacting a compound described herein with an enzyme, such as a histone deacetylase enzyme or a histone acetyltransferase enzyme, and an endopeptidase that recognizes basic amino acids to release the reporter moiety which may be subsequently detected.Type: ApplicationFiled: September 9, 2011Publication date: March 15, 2012Applicant: PROMEGA CORPORATIONInventors: Thomas Kirkland, Andrew L. Niles, Martha O'Brien, Carolyn Woodroofe Hitko
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Patent number: 8071328Abstract: A sensitive bioluminescent assay to detect proteases including caspases, trypsin and tryptase is provided.Type: GrantFiled: February 16, 2010Date of Patent: December 6, 2011Assignee: Promega CorporationInventors: Martha O'Brien, Keith V. Wood, Dieter Klaubert, William Daily
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Publication number: 20110081670Abstract: A method and kit are provided for enhancing the tolerance of an assay reagent to compounds in an assay sample, the assay reagent including a luciferase enzyme. The method includes contacting the luciferase with a tolerance enhancement agent in an amount sufficient to substantially protect luciferase enzyme activity from interference of the compound and minimize interference by at least about 10% relative to an assay not having tolerance enhancement agent.Type: ApplicationFiled: June 21, 2010Publication date: April 7, 2011Applicant: PROMEGA CORPORATIONInventors: Erika Hawkins, James J. Cali, Samuel Kin Sang Ho, Martha A. O'Brien, Richard Somberg, Robert F. Bulleit, Keith V. Wood
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Publication number: 20100249427Abstract: A sensitive bioluminescent assay to detect proteases including caspases, trypsin and tryptase is provided.Type: ApplicationFiled: February 16, 2010Publication date: September 30, 2010Applicant: PROMEGA CORPORATIONInventors: Martha O'Brien, Keith V. Wood, Dieter Klaubert, William Daily
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Patent number: 7741067Abstract: A method and kit is provided for enhancing the tolerance of an assay reagent to compounds in an assay sample, the assay reagent including a luciferase enzyme. The method includes contacting the luciferase with a tolerance enhancement agent in an amount sufficient to substantially protect luciferase enzyme activity from interference of the compound and minimize interference by at least about 10% relative to an assay not having tolerance enhancement agent.Type: GrantFiled: December 23, 2003Date of Patent: June 22, 2010Assignee: Promega CorporationInventors: Erika Hawkins, James J. Cali, Samuel Kin Sang Ho, Martha A. O'Brien, Richard Somberg, Robert F. Bulleit, Keith V. Wood
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Patent number: 7666987Abstract: A sensitive bioluminescent assay to detect proteases including caspases is provided which employs an aminoluciferin or a carboxy-terminal protected derivative thereof covalently linked via a peptide bond to a substrate for a caspase or an aminoluciferin or a carboxy-terminal protected derivative thereof covalently linked via a peptide bond to a peptide substrate comprising aspartate that is specifically cleaved by a protease specific for the substrate.Type: GrantFiled: February 3, 2006Date of Patent: February 23, 2010Assignee: Promega CorporationInventors: Martha O'Brien, Keith V. Wood, Dieter Klaubert, William Daily
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Patent number: 7384758Abstract: A sensitive bioluminescent assay to detect proteases, including caspases and proteases that specifically cleave a peptide substrate having aspartate, is provided. In one embodiment, the assay employs an aminoluciferin or a carboxy-terminal protected derivative thereof covalently linked via a peptide bond to a substrate for a caspase or an aminoluciferin or a carboxy-terminal protected derivative thereof covalently linked via a peptide bond to a peptide substrate comprising aspartate that is specifically cleaved by a protease specific for the substrate.Type: GrantFiled: February 2, 2006Date of Patent: June 10, 2008Assignee: Promeaga CorporationInventors: Martha O'Brien, Keith V. Wood, Dieter Klaubert, William Daily
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Patent number: 7148030Abstract: A sensitive bioluminescent assay to detect proteases including caspases, trypsin and tryptase is provided.Type: GrantFiled: January 31, 2003Date of Patent: December 12, 2006Assignee: Promega CorporationInventors: Martha O'Brien, Keith V. Wood, Dieter Klaubert, William Daily
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Publication number: 20060183177Abstract: A sensitive bioluminescent assay to detect proteases including caspases, trypsin and tryptase is provided.Type: ApplicationFiled: February 2, 2006Publication date: August 17, 2006Inventors: Martha O'Brien, Keith Wood, Dieter Klaubert, William Daily