Patents by Inventor Martha R. Stampfer
Martha R. Stampfer has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
-
Patent number: 11293008Abstract: A method of growing primary human epithelial cells, in particular human epithelial cells using a basal formula containing individual (a) amino acids, (b) vitamins, (c) trace elements, and (d) other organics such as linoleic acid. The basal medium may be a mixture of amino acids, vitamins, and salts that constitute the basic media that is used to culture epithelial cells over a number of population doublings, e.g., over at least one week, while maintaining a normal phenotype and exerting low stress on the cultured cells, and maintaining lineage heterogeneity.Type: GrantFiled: September 23, 2016Date of Patent: April 5, 2022Assignee: The Regents of the University of CaliforniaInventors: Mark A. LaBarge, Martha R. Stampfer, James C. Garbe
-
Patent number: 10870851Abstract: Aspects of the present disclosure include RNAs that confer a mortal phenotype and nucleic acids encoding same. Liposomes, recombinant cells, and pharmaceutical compositions that include the RNAs or nucleic acids encoding same are also provided. Further provided are methods involving quantifying MORT RNAs and/or determining the methylation status of the MORT promoter, as well as methods that employ the RNAs or nucleic acids encoding same.Type: GrantFiled: October 5, 2016Date of Patent: December 22, 2020Assignees: The Regents of the University of California, The Arizona Board of Regents on Behalf of the University of ArizonaInventors: Martha R. Stampfer, James C. Garbe, Lukas Vrba, Bernard W. Futscher
-
Patent number: 10689652Abstract: Methods for inducing non-clonal immortalization of normal epithelial cells by directly targeting the two main senescence barriers encountered by cultured epithelial cells. In finite lifespan pre-stasis human mammary epithelial cells (HMEC), the stress-associated stasis barrier was bypassed, and in post-stasis HMEC, the replicative senescence barrier, a consequence of critically shortened telomeres, was bypassed. Early passage non-clonal immortalized lines exhibited normal karyotypes. Methods of efficient HMEC immortalization, in the absence of “passenger” genomic errors, should facilitate examination of telomerase regulation and immortalization during human carcinoma progression, methods for screening for toxic and environmental effect on progression, and the development of therapeutics targeting the process of immortalization.Type: GrantFiled: September 2, 2016Date of Patent: June 23, 2020Assignee: The Regents of the University of CaliforniaInventors: Martha R. Stampfer, James C. Garbe
-
Publication number: 20190352606Abstract: A method of growing primary human epithelial cells, in particular human epithelial cells using a basal formula containing individual (a) amino acids, (b) vitamins, (c) trace elements, and (d) other organics such as linoleic acid. The basal medium may be a mixture of amino acids, vitamins, and salts that constitute the basic media that is used to culture epithelial cells over a number of population doublings, e.g., over at least one week, while maintaining a normal phenotype and exerting low stress on the cultured cells, and maintaining lineage heterogeneity.Type: ApplicationFiled: September 23, 2016Publication date: November 21, 2019Inventors: Mark A. LaBarge, Martha R. Stampfer, James C. Garbe
-
Publication number: 20180298382Abstract: Aspects of the present disclosure include RNAs that confer a mortal phenotype and nucleic acids encoding same. Liposomes, recombinant cells, and pharmaceutical compositions that include the RNAs or nucleic acids encoding same are also provided. Further provided are methods involving quantifying MORT RNAs and/or determining the methylation status of the MORT promoter, as well as methods that employ the RNAs or nucleic acids encoding same.Type: ApplicationFiled: October 5, 2016Publication date: October 18, 2018Inventors: Martha R. Stampfer, James C. Garbe, Lukas Vrba, Bernard W. Futscher
-
Publication number: 20170130232Abstract: Methods for inducing non-clonal immortalization of normal epithelial cells by directly targeting the two main senescence barriers encountered by cultured epithelial cells. In finite lifespan pre-stasis human mammary epithelial cells (HMEC), the stress-associated stasis barrier was bypassed, and in post-stasis HMEC, the replicative senescence barrier, a consequence of critically shortened telomeres, was bypassed. Early passage non-clonal immortalized lines exhibited normal karyotypes. Methods of efficient HMEC immortalization, in the absence of “passenger” genomic errors, should facilitate examination of telomerase regulation and immortalization during human carcinoma progression, methods for screening for toxic and environmental effect on progression, and the development of therapeutics targeting the process of immortalization.Type: ApplicationFiled: September 2, 2016Publication date: May 11, 2017Applicants: The Regents of the University of California, The Arizona Board of Regents on Behalf of the University of ArizonaInventors: Martha R. Stampfer, James C. Garbe, Lukas Vrba, Bernard W. Futscher
-
Patent number: 9376662Abstract: Cell culture media formulations for culturing human epithelial cells are herein described. Also described are methods of increasing population doublings in a cell culture of finite life span human epithelial cells and prolonging the life span of human cell cultures. Using the cell culture media disclosed alone and in combination with addition to the cell culture of a compound associated with anti-stress activity achieves extended growth of pre-stasis cells and increased population doublings and life span in human epithelial cell cultures.Type: GrantFiled: February 20, 2015Date of Patent: June 28, 2016Assignee: The Regents of the University of CaliforniaInventors: Martha R. Stampfer, James C. Garbe
-
Publication number: 20150247121Abstract: Cell culture media formulations for culturing human epithelial cells are herein described. Also described are methods of increasing population doublings in a cell culture of finite life span human epithelial cells and prolonging the life span of human cell cultures. Using the cell culture media disclosed alone and in combination with addition to the cell culture of a compound associated with anti-stress activity achieves extended growth of pre-stasis cells and increased population doublings and life span in human epithelial cell cultures.Type: ApplicationFiled: February 20, 2015Publication date: September 3, 2015Inventors: Martha R. Stampfer, James C. Garbe
-
Publication number: 20150225696Abstract: Methods for inducing non-clonal immortalization of normal epithelial cells by directly targeting the two main senescence barriers encountered by cultured epithelial cells. In human mammary epithelial cells (HMEC), the stress-associated stasis barrier was bypassed and the replicative senescence barrier, a consequence of critically shortened telomeres, was bypassed in post-stasis HMEC. Early passage non-clonal immortalized lines exhibited normal karyotypes. Methods of efficient HMEC immortalization, in the absence of “passenger” genomic errors, should facilitate examination of telomerase regulation, immortalization during human carcinoma progression, and methods for screening for toxic and environmental effect on progression.Type: ApplicationFiled: October 2, 2014Publication date: August 13, 2015Applicants: The Regents of the University of California, THE ARIZONA BOARD OF REGENTS ON BEHALF OF THE UNIVERSITY OF ARIZONAInventors: Martha R. Stampfer, James C. Garbe, Lukas Vrba, Bernard W. Futscher
-
Patent number: 8962325Abstract: Cell culture media formulations for culturing human epithelial cells are herein described. Also described are methods of increasing population doublings in a cell culture of finite life span human epithelial cells and prolonging the life span of human cell cultures. Using the cell culture media disclosed alone and in combination with addition to the cell culture of a compound associated with anti-stress activity achieves extended growth of pre-stasis cells and increased population doublings and life span in human epithelial cell cultures.Type: GrantFiled: September 30, 2008Date of Patent: February 24, 2015Assignee: The Regents of the University of CaliforniaInventors: Martha R. Stampfer, James C. Garbe
-
Publication number: 20100022000Abstract: Cell culture media formulations for culturing human epithelial cells are herein described. Also described are methods of increasing population doublings in a cell culture of finite life span human epithelial cells and prolonging the life span of human cell cultures. Using the cell culture media disclosed alone and in combination with addition to the cell culture of a compound associated with anti-stress activity achieves extended growth of pre-stasis cells and increased population doublings and life span in human epithelial cell cultures.Type: ApplicationFiled: September 30, 2008Publication date: January 28, 2010Applicant: The Regents of the University of CaliforniaInventors: Martha R. Stampfer, James C. Garbe
-
Patent number: 4808532Abstract: Substantially genetically stable continuous human cell lines derived from normal human mammary epithelial cells (HMEC) and processes for making and using the same. In a preferred embodiment, the cell lines are derived by treating normal human mammary epithelial tissue with a chemical carcinogen such as benzo[a]pyrene. The novel cell lines serve as useful substrates for elucidating the potential effects of a number of toxins, carcinogens and mutagens as well as of the addition of exogenous genetic material. The autogenic parent cells from which the cell lines are derived serve as convenient control samples for testing. The cell lines are not neoplastically transformed, although they have acquired several properties which distinguish them from their normal progenitors.Type: GrantFiled: July 1, 1985Date of Patent: February 28, 1989Assignee: The United States of America as represented by the United States Department of EnergyInventor: Martha R. Stampfer
-
Patent number: 4423145Abstract: Methods are disclosed for isolating and culturing human mammary epithelial cells of both normal and malignant origin. Tissue samples are digested with a mixture including the enzymes collagenase and hyaluronidase to produce clumps of cells substantially free from stroma and other undesired cellular material. Growing the clumps of cells in mass culture in an enriched medium containing particular growth factors allows for active cell proliferation and subculture. Clonal culture having plating efficiencies of up to 40% or greater may be obtained using individual cells derived from the mass culture by plating the cells on appropriate substrates in the enriched media. The clonal growth of cells so obtained is suitable for a quantitative assessment of the cytotoxicity of particular treatment. An exemplary assay for assessing the cytotoxicity of the drug adriamycin is presented.Type: GrantFiled: May 7, 1981Date of Patent: December 27, 1983Inventors: Martha R. Stampfer, Helene S. Smith, Adeline J. Hackett