Abstract: The present invention concerns a method for detecting a molecular recognition by electrochemiluminescence. Said method comprises the steps consisting in a) depositing an electronically conductive film on a support and attaching on said film a sensor molecule in order to obtain a test support; b) subjecting the sample to be tested to a protocol of marking the target molecule with an electrochemiluminescent marker; c) bringing the marked target into contact with the test support; d) rinsing the test support; and e) subjecting the test support rinsed in step d) to a reading of the electrochemiluminescence signal triggered by a direct, or indirect, electronic transfer between the target and the support via the electronically conductive film.