Abstract: A method for detecting a target nucleic acid sequence in a sample, by subjecting it to an amplification reaction and taking continuous electrochemical measurements on it during the reaction. The method can be used to determine whether an amplification reaction has taken place, to quantitate the amount of target in the sample or to determine sequence characteristics. Also disclosed is apparatus for use in the method, comprising (i) an amplification reaction vessel which comprises an electrochemical cell, (ii) means for taking continuous electrochemical measurements on a sample contained in the vessel and (iii) temperature control and measurement means, wherein the electrochemical cell comprises an element formed from an electrically conducting plastics material such as a polymer loaded with an electrically conducting material. Further disclosed is a reaction vessel for use in the apparatus, a probe for use in the method and a kit for effecting the method.

Abstract: A method for detecting the presence of a target nucleic acid sequence in a sample is provided. The method comprises subjecting the sample to an amplification reaction to obtain an amplification product where the target nucleic acid sequence is present using a set of nucleotides, at least one of which is fluorescently labelled. The amplification product is contacted with a probe under conditions in which the probe will hybridise to the target sequence. The probe comprises a reactive molecule which is capable of absorbing fluorescence energy from or donating fluorescent energy to the fluorescent labelled nucleotide. The fluorescence of the sample is monitored and related to the presence of the target nucleic acid sequence. The method can be used to quantitate the amount of target nucleic acid in the sample as well as to determine sequence characteristics. Kits for effecting the method are also provided.