Abstract: This invention provides for an improved method of stabilizing freeze dried bacterial extracts with a carbohydrate lyoprotectant such that the extracts can be for use in cell free protein synthesis. Also provided herein are formulations for stable, freeze dried bacterial extracts that when stored at room temperature retain at least about 70% protein synthesis activity compared to undried frozen bacterial extracts.

Abstract: This invention provides for an improved method of stabilizing freeze dried bacterial extracts with a carbohydrate lyoprotectant such that the extracts can be for use in cell free protein synthesis. Also provided herein are formulations for stable, freeze dried bacterial extracts that when stored at room temperature retain at least about 70% protein synthesis activity compared to undried frozen bacterial extracts.

Abstract: This invention provides for an improved method of stabilizing freeze dried bacterial extracts with a carbohydrate lyoprotectant such that the extracts can be for use in cell free protein synthesis. Also provided herein are formulations for stable, freeze dried bacterial extracts that when stored at room temperature retain at least about 70% protein synthesis activity compared to undried frozen bacterial extracts.

Abstract: This invention provides for an improved method of stabilizing freeze dried bacterial extracts with a carbohydrate lyoprotectant such that the extracts can be for use in cell free protein synthesis. Also provided herein are formulations for stable, freeze dried bacterial extracts that when stored at room temperature retain at least about 70% protein synthesis activity compared to undried frozen bacterial extracts.

Abstract: The use of two tandem microfiltration (MF) steps in a process for making recombinant insulin is described. The two MF steps in a single downstream purification unit operation reduce both soluble and insoluble impurities and exchange the insulin product into a suitable buffer for downstream purification.

Abstract: An HME has a housing (10) moulded in two parts (26 and 27) of a thick, thermally-insulative foamed plastics to form an enlarged central region (13) and opposite end walls (24 and 25). Separate patient and machine end couplings (11 and 12) are bonded to openings (37 and 47) in the end walls. The two housing parts (26 and 27) are bonded end to end about an HME element (14) formed by a strip of treated corrugated paper wound about a support sleeve (50).

Abstract: The use of two tandem microfiltration (MF) steps in a process for making recombinant insulin is described. The two MF steps in a single downstream purification unit operation reduce both soluble and insoluble impurities and exchange the insulin product into a suitable buffer for downstream purification.

Abstract: This invention provides for an improved method of stabilizing freeze dried bacterial extracts with a carbohydrate lyoprotectant such that the extracts can be for use in cell free protein synthesis. Also provided herein are formulations for stable, freeze dried bacterial extracts that when stored at room temperature retain at least about 70% protein synthesis activity compared to undried frozen bacterial extracts.

Abstract: There is provided an apparatus and method for monitoring pulsation of a milking system in a dairy facility. The milking system includes vacuum hoses that extend from a vacuum source to a milking claw. The pressure in each vacuum hose is changed by a pulsator, with the vacuum hoses being out of phase with one another. The pressure sensors are coupled to the respective vacuum hoses, which sensors monitor the pressure pulsations to ensure that the pulsator is operating satisfactorily. The pressure sensors are coupled to the vacuum hoses by way of passageways or hoses. A bypass is provided between the passageways so as to provide air circulation in the otherwise dead air space in these passageways. The bypass is small so as to not interfere with the pressure measurements by the sensors and is located in close proximity to the pressure sensors so as to minimize the amount of dead air space.

Abstract: There is disclosed a process for purifying a natural product using a two-phase, multi-solvent system followed by vacuum concentration and back extraction. The method allows for the removal of impurities by controlling the polarity balance of a two-phase system by manipulating the proportions of the four solvents and subsequently the relative distribution of the product versus the impurities.

Abstract: A pulsator provides alternating vacuum pressure and atmospheric pressure to an elastomeric liner in a sleeve of a milking claw in a dairy barn. The purpose of pulsation is to alternate the cow teat between a milking phase and a rest phase. The invention monitors the operation of the pulsator to determine if the pulsator is operating satisfactorily. The invention records data during a calibration mode from the pulsator in normal operation. The data that is recorded is the duration of the milking phase and the rest phase and the associated pressures. During pulsator operation, the invention measures the pulsation between atmospheric and vacuum pressures provided to the milking claw and compares this measured data to the recorded calibration data. If the pulsator is not found to be operating within predetermined tolerances, then an indication of a pulsator malfunction is provided, typically in the form of a light located in the milking pen.

Abstract: A pulsator provides alternating vacuum pressure and atmospheric pressure to an elastomeric liner in a sleeve of a milking claw in a dairy barn. The purpose of pulsation is to alternate the cow teat between a milking phase and a rest phase. The invention monitors the operation of the pulsator to determine if the pulsator is operating satisfactorily. The invention records data during a calibration mode from the pulsator in normal operation. The data that is recorded is the duration of the milking phase and the rest phase and the associated pressures. During pulsator operation, the invention measures the pulsation between atmospheric and vacuum pressures provided to the milking claw and compares this measured data to the recorded calibration data. If the pulsator is not found to be operating within predetermined tolerances, then an indication of a pulsator malfunction is provided, typically in the form of a light located in the milking pen.

Abstract: There is disclosed a process for purifying a natural product using a two-phase, multi-solvent system followed by vacuum concentration and back extraction. The method allows for the removal of impurities by controlling the polarity balance of a two-phase system by manipulating the proportions of the four solvents and subsequently the relative distribution of the product versus the impurities.