Abstract: The invention relates, in general, to methods of processing Lignocellulosic biomass to fermentable sugars and to methods that rely on hydrothermal pretreatment. Xylose monomer yields comparable to those achieved using two-stage pretreatments can be achieved from soft Lignocellulosic biomass feedstocks by pretreasting to very low severity in a single-stage pressurized hydrothermal pretreatment, followed by enzymatic hydrolysis to release xylose retained in the solid state. In some embodiments, pretreated biomass is separated into a solid fraction and a liquid fraction, the solid fraction subjected to enzymatic hydrolysis, and the separated liquid fraction subsequently mixed with the hydrolysed solid fraction.

Abstract: The invention relates, in general, to methods of processing Lignocellulosic biomass to fermentable sugars and to methods that rely on hydrothermal pretreatment. Xylose monomer yields comparable to those achieved using two-stage pretreatments can be achieved from soft Lignocellulosic biomass feedstocks by pretreasting to very low severity in a single-stage pressurized hydrothermal pretreatment, followed by enzymatic hydrolysis to release xylose retained in the solid state. In some embodiments, pretreated biomass is separated into a solid fraction and a liquid fraction, the solid fraction subjected to enzymatic hydrolysis, and the separated liquid fraction subsequently mixed with the hydrolysed solid fraction.

Abstract: A method of producing biomass degradation products from soft biomass is disclosed, the method comprising the steps of—providing a soft biomass, pretreating the soft biomass in a pretreatment step at a pressure below 2 bar by heating the soft biomass to at a pretreatment temperature between 65 and 100 degrees Celsius to obtain a pretreated biomass, hydrolyzing the pretreated biomass in a first hydrolyzation step to obtain a biomass hydrolysate, and posttreating the biomass hydrolysate in a pressurized posttreatment step by heating the biomass hydrolysate to a posttreatment temperature above 150 degrees Celsius to obtain a posttreated biomass, hydrolyzing the posttreated biomass in a second hydrolyzation step, wherein biogas is obtained from at least the first hydrolyzation step or the second hydrolyzation step.

Abstract: A method for processing biomass digestate is disclosed. The method comprises the steps of—providing a biomass digestate (BD), —subjecting the biomass digestate to a separation step (SEP) into a liquid fraction (LF) and a solid biomass digestate fraction (SBD), —subjecting the solid biomass digestate fraction (SBD) to an acidic wash step (AW) at a pressure below 200 kPa (2 bar) to obtain a washed biomass digestate (WBD), —separating (SEP) the washed biomass digestate (WBD) into a liquid wash fraction (LW) and a solid wash fraction (SW), —posttreating (POST) the solid wash fraction (SW) to obtain a posttreated solid wash fraction (PSW) and—anaerobic digesting (AD) the posttreated solid wash fraction (PSW) to obtain a posttreated biomass digestate (PBD) wherein the amount of phosphorus in the solid wash fraction (SW) is reduced compared to amount of phosphorus in the solid biomass digestate fraction (SBD).

Abstract: The invention relates, in general, to methods of processing Lignocellulosic biomass to fermentable sugars and to methods that rely on hydrothermal pretreatment. Xylose monomer yields comparable to those achieved using two-stage pretreatments can be achieved from soft Lignocellulosic biomass feedstocks by pretreasting to very low severity in a single-stage pressurized hydrothermal pretreatment, followed by enzymatic hydrolysis to release xylose retained in the solid state. In some embodiments, pretreated biomass is separated into a solid fraction and a liquid fraction, the solid fraction subjected to enzymatic hydrolysis, and the separated liquid fraction subsequently mixed with the hydrolysed solid fraction.

Abstract: The invention relates, in general, to methods of processing lignocellulosic biomass to fermentable sugars and to methods that rely on hydrothermal pretreatment. Xylose monomer yields comparable to those achieved using two-stage pretreatments can be achieved from soft lignocellulosic biomass feedstocks by pretreating to very low severity in a single-stage pressurized hydrothermal pretreatment, followed by enzymatic hydrolysis to release xylose retained in the solid state. In some embodiments, pretreated biomass is separated into a solid fraction and a liquid fraction, the solid fraction subjected to enzymatic hydrolysis, and the separated liquid fraction subsequently mixed with the hydrolysed solid fraction.

Abstract: A method for providing a C5/C6 product from a lignocellulosic material is disclosed, said method comprising the steps: (i) pretreatment of the lignocellulosic material; (ii) solid/liquid separation of the pretreated lignocellulosic material from step (a) into a first solid fraction and a first liquid fraction; (iii) enzymatic fiber hydrolysis of said first solid fraction from step (b) by use of an enzyme composition capable of degrading lignocellulosic material, thereby providing a C5/C6 fiber slurry comprising C5 and/or C6 sugars; (iv) solid/liquid separation of the C5/C6 fiber slurry from step (c) into a second solid fraction and a second liquid fraction; and optionally (v) combining said first liquid fraction and said second liquid fraction for enzymatic mixed sugar hydrolysis (MSH), whereby a MSH C5/C6 product is provided.

Abstract: Methods of processing lignocellulosic biomass to fermentable sugars are provided which rely on hydrothermal pretreatment. Soft lignocellulosic biomass feedstock is pretreated in a single-stage pressurized hydrothermal pretreatment to very low severity. The pre-treated biomass is hydrolysed, typically as a whole slurry, using enzymatic hydrolysis catalysed by an enzyme mixture comprising endoglucanase, exoglucanase, ?-glucosidase, endoxylanase, and ?-xylosidase activities at activity levels in nkat/g glucan of endoglucanase of at least 1100, exoglucanase of at least 280, ?-glucosidase of at least 3000, endoxylanase of at least 1400, and ?-xylosidase of at least 75, so as to produce a hydrolysate in which the yield of C5 monomers is at least 55% of the original xylose and arabinose content of the feedstock prior to pretreatment.

Abstract: The current invention concerns methods and products related to the production of alcohol from sugar cane and/or sweet sorghum with integration of 1st and 2nd generation (1G/2G) biorefining, thus comprising the integrated conversion of primary and secondary soft lignocellulosic biomass. In particular, methods of processing sugar cane and/or sweet sorghum feedstock are disclosed, said methods comprising the steps of providing raw juice from the feedstock, recovering a residual bagasse, pretreating the bagasse and mixing it with some quantity of raw juice, and hydrolyzing the pretreated bagasse enzymatically.

Abstract: Methods of processing lignocellulosic biomass to fermentable sugars are provided which rely on hydrothermal pretreatment. Soft lignocellulosic biomass feedstock is pretreated in a single-stage pressurized hydrothermal pretreatment to very low severity. The pre-treated biomass is hydrolysed, typically as a whole slurry, using enzymatic hydrolysis catalysed by an enzyme mixture comprising endoglucanase, exoglucanase, ?-glucosidase, endoxylanase, and ?-xylosidase activities at activity levels in nkat/g glucan of endoglucanase of at least 1100, exoglucanase of at least 280, ?-glucosidase of at least 3000, endoxylanase of at least 1400, and ?-xylosidase of at least 75, so as to produce a hydrolysate in which the yield of C5 monomers is at least 55% of the original xylose and arabinose content of the feedstock prior to pretreatment.

Abstract: The invention relates, in general, to methods of processing lignocellulosic biomass to fermentable sugars and to methods that rely on hydrothermal pretreatment. Xylose monomer yields comparable to those achieved using two-stage pretreatments can be achieved from soft lignocellulosic biomass feedstocks by pretreating to very low severity in a single-stage pressurized hydrothermal pretreatment, followed by enzymatic hydrolysis to release xylose retained in the solid state. In some embodiments, pretreated biomass is separated into a solid fraction and a liquid fraction, the solid fraction subject to enzymatic hydrolysis, and the separated liquid fraction subsequently mixed with the hydrolysed solid fraction.

Abstract: Methods are provided for improved processing of lignocellulosic biomass. Hydrothermally pretreated lignocellulosic biomass is subject to separate hydrolysis and fermentation (SHF) or prehydrolysed and subject to simultaneous saccharification and fermentation (SSF) at high initial loadings of cellulase enzymes, at least 15 FPU/g DM. The cellulase enzymes are subsequently recycled and used in subsequent hydrolysis cycles along with a lower dose supplementation of fresh enzyme. Loss of enzyme activity between hydrolysis cycles is offset by improved overall process advantage.