Abstract: The present invention relates to a chromatography ligand, which comprises Domain C from Staphylococcus protein A (SpA), or a functional fragment or variant thereof. The chromatography ligand presents an advantageous capability of withstanding harsh cleaning in place (CIP) conditions, and is capable of binding Fab fragments of antibodies. The ligand may be provided with a terminal coupling group, such as arginine or cysteine, to facilitate its coupling to an insoluble carrier such as beads or a membrane. The invention also relates to a process of using the ligand in isolation of antibodies, and to a purification protocol which may include washing steps and/or regeneration with alkali.

Abstract: The present invention relates to a chromatography ligand, which comprises Domain C from Staphylococcus protein A (SpA), or a functional fragment or variant thereof. The chromatography ligand presents an advantageous capability of withstanding harsh cleaning in place (CIP) conditions, and is capable of binding Fab fragments of antibodies. The ligand may be provided with a terminal coupling group, such as arginine or cysteine, to facilitate its coupling to an insoluble carrier such as beads or a membrane. The invention also relates to a process of using the ligand in isolation of antibodies, and to a purification protocol which may include washing steps and/or regeneration with alkali.

Abstract: A method and apparatus for dispensing a predetermined fixed amount of pre-stretched packaging material based upon load girth is disclosed. A non-rotating ring carries a belt driven by a motor. A packaging material dispenser is mounted on a rotating ring, and the rotating ring may include a pulley that connects to the band, such that the rotating ring is driven by the drive belt. Based upon the girth of the load to be wrapped, an amount of pre-stretched packaging material to be dispensed for at least a portion of a revolution made by the rotating ring is determined. Good wrapping performance in terms of load containment (wrap force) and optimum packaging material use is obtained by dispensing a length of pre-stretched packaging material that is between approximately 90% and approximately 120% of load girth. Once the amount of packaging material to be dispensed per revolution is determined, a ratio of rotating ring drive to final pre-stretch surface speed (i.e.

Abstract: A system and method for monitoring a computer, particularly a pay-per-use computer, uses an isolated computing environment or supervisor. The isolated computing environment boots prior to any boot device associated with an operating system, runs concurrently with the operating system and monitors and measures the computer in operation. Once the isolated computing environment determines the computer is not in compliance with the required policies, the isolated computing environment may either impose an impediment to use such as slowing clock speed or completely disable the operating system. The user may have to return the computer to a service provider to restore it from the offending condition and reset the computer to an operational state.

Abstract: An apparatus and method for wrapping a palletized load are provided. The apparatus preferably includes a film dispenser for dispensing a film web, at least one drive down roller configured to engage the width of the film web, and at least one roping mechanism. The at least one roping mechanism may be a cable rolling element configured to roll a portion of the film web into a rolled cable of film. The apparatus may also include an assembly for providing relative rotation between the load and the dispenser to wrap a roped portion of the film web around a base of the load/top portion of a pallet supporting the load.

Abstract: The present invention relates to a process of enriching one target compound from a liquid, which process comprises at least one step of isolation performed by differentially partitioning between two aqueous phases. In the present invention the phases are formed by adding a thermally responsive, self-associating (i.e. clouding) hydrophilic polymer, and if needed some additional salts, to an aqueous biotechnical solution (such as a fermentation sample or bioseparation process stream) under thermal and other conditions where the solution separates into a one polymer, two-phase system with one phase enriched in the polymer. The target compound is to be found in the phase not enriched in the polymer, while a significant though varying percentage of contaminants may differentially partition to the phase interface or the polymer enriched phase.

Abstract: An apparatus and method for wrapping a palletized load are provided. The apparatus preferably includes a film dispenser for dispensing a film web, at least one drive down roller configured to engage the width of the film web, and at least one roping mechanism. The at least one roping mechanism may be a cable rolling element configured to roll a portion of the film web into a rolled cable of film. The apparatus may also include an assembly for providing relative rotation between the load and the dispenser to wrap a roped portion of the film web around a base of the load/top portion of a pallet supporting the load.

Abstract: The present disclosure provides a method and apparatus for dispensing a predetermined fixed amount of pre-stretched packaging material based upon load girth. A non-rotating ring carries a belt driven by a motor. A packaging material dispenser is mounted on a rotating ring, and the rotating ring may include a pulley that connects to the band, such that the rotating ring is driven by the drive belt. Based upon the girth of the load to be wrapped, an amount of pre-stretched packaging material to be dispensed for at least a portion of a revolution made by the rotating ring is determined. Good wrapping performance in terms of load containment (wrap force) and optimum packaging material use is obtained by dispensing a length of pre-stretched packaging material that is between approximately 90% and approximately 120% of load girth. Once the amount of packaging material to be dispensed per revolution is determined, a ratio of rotating ring drive to final pre-stretch surface speed (i.e.

Abstract: The present invention relates to a simulated moving bed process, wherein at least one adsorbent is washed after binding of target compound and wherein the outlet of wash liquid from the adsorbent is subsequently passed onto another adsorbent for binding of target compound removed by the washing. In one embodiment, the method comprises binding of at least one target compound using three or more adsorbents connected in series and elution of target compound from said three adsorbents. After the binding to an adsorbent, wash liquid is passed across the adsorbent to recover desorbed and/or unbound target compound, and the outlet of such wash liquid is directed to the adsorbent after the next in the series, to which no feed has yet been added. The target compound is recovered by eluting target compound from the washed adsorbents.

Abstract: Systems and methods are provided for linking a web identity and a portable device to provide web access to a user from a vehicle. An example system includes a presence agent that may be configured to validate the presence of the portable device in the vehicle by recognizing a device identifier saved on the portable device. After the presence of the portable device is validated, a security manager may receive a user identifier, and to verify if the user identifier corresponds with a known user value. Then, the security manager may send a user identity secure proxy to a credential manager. The credential manager may be configured to receive the user identity secure proxy, and in response, pass one or more credentials to a computing device onboard the vehicle. The credentials may indicate that the user is authorized to access one or more web services from the computing device.

Abstract: Systems and methods are provided for delivering contextual advertising to a vehicle. An example system may include a profiler module executed by an onboard computing device of the vehicle, and configured to aggregate vehicle event data from a plurality of vehicle-based event sources, and to develop user profile data based on the vehicle event data. A communication agent may also be executed by the onboard computing device, and configured to transmit the user profile data to an advertising service executed on an advertising server via a communication network. The communication agent may also be configured to retrieve an advertisement from the advertising service. The advertisement may be selected based on content of the user profile data. The system may also include an interface module executed by the onboard computing device, and configured to present the advertisement via a display, and/or speaker associated with the onboard computing device.

Abstract: Embodiments related to network video messaging are disclosed. One disclosed embodiment provides a method that comprises receiving a video message from a client application of a source client; associating a navigation link with the video message; transmitting a notification message to the recipient client including the navigation link; receiving a retrieval request from the recipient client to access the video content via the navigation link; and transmitting the video content to the recipient client responsive to receiving the retrieval request by providing a persistent download of the video content from the storage server if the download condition indicates that a persistent download of the video content is permissible; and providing a transient download of the video content to the recipient client while prohibiting a persistent download of the video content if the download condition indicates that a persistent download of the video content is not permissible.

Abstract: The present invention provides a method and apparatus for dispensing a predetermined fixed amount of pre-stretched packaging material based upon load girth. A non-rotating ring carries a belt driven by a motor. A packaging material dispenser is mounted on a rotating ring, and the rotating ring may include a pulley that connects to the band, such that the rotating ring is driven by the drive belt. Based upon the girth of the load to be wrapped, an amount of pre-stretched packaging material to be dispensed for each revolution made by the rotating ring is determined. Good wrapping performance in terms of load containment (wrap force) and optimum packaging material use is obtained by dispensing a length of pre-stretched packaging material that is between approximately 90% and approximately 120% of load girth. Once the amount of packaging material to be dispensed per revolution is determined, a ratio of rotating ring drive to final pre-stretch surface speed (i.e.

Abstract: The present invention relates to a simulated moving bed process, wherein at least one adsorbent is washed after binding of target compound and wherein the outlet of wash liquid from the adsorbent is subsequently passed onto another adsorbent for binding of target compound removed by the washing. In one embodiment, the method comprises binding of at least one target compound using three or more adsorbents connected in series and elution of target compound from said three adsorbents. After the binding to an adsorbent, wash liquid is passed across the adsorbent to recover desorbed and/or unbound target compound, and the outlet of such wash liquid is directed to the adsorbent after the next in the series, to which no feed has yet been added. The target compound is recovered by eluting target compound from the washed adsorbents.

Abstract: The present invention relates to a chromatography ligand, which comprises Domain C from Staphylococcus protein A (SpA), or a functional fragment or variant thereof. The chromatography ligand presents an advantageous capability of withstanding harsh cleaning in place (CIP) conditions, and is capable of binding Fab fragments of antibodies. The ligand may be provided with a terminal coupling group, such as arginine or cysteine, to facilitate its coupling to an insoluble carrier such as beads or a membrane. The invention also relates to a process of using the ligand in isolation of antibodies, and to a purification protocol which may include washing steps and/or regeneration with alkali.

Abstract: A film dispenser for a stretch wrapping apparatus is provided. The film dispenser includes a pre-stretch assembly having a top portion mounted to and extending downwardly from a drive plate of a roll carriage frame. The pre-stretch assembly includes two pre-stretch rollers having first ends connected to the roll carriage frame and second ends connected to one another. The pre-stretch rollers may be coated. The pre-stretch assembly further includes a cantilevered idle roller mounted to the drive plate and positioned between the pre-stretch rollers. A film path is defined by the space between the pre-stretch rollers and the idle roller. The film path is easily accessible via the bottom end of the pre-stretch assembly.

Abstract: A golf training system and method wherein a device comprising a straight, elongated arm having an axis, a first proximal end, and a second distal end; a wrist bar connected to the elongated arm at a point near the first proximal end, extending perpendicularly from the straight, elongated arm at a right angle to the axis of the straight, elongated arm; a first green laser enclosed by the straight, elongated arm adapted to emit a green laser beam from the first proximal end along the axis; and a second green laser enclosed by the straight, elongated arm adapted to emit a green laser beam from the second distal end is used to train on plane swing, proper wrist angulations, proper hip turn, proper shoulder turn, and a proper putting motion.

Abstract: User persona content targeting is described. In an embodiment, a targeted content system includes a client account that corresponds to a user of computing-based client device(s), and the client account is associated with multiple personas that are each a different on-line representation of the user. A persona system initiates content being targeted to the user via a computing-based client device when the user is represented on-line as a first persona, and then initiates different content being targeted to the user via the computing-based client device when the user is represented on-line as a second persona.

Abstract: An application program interface (API) for sending and receiving batches of requests and responses has a batch request call for exchanging one or more request items between a provider system and a subscription system and a batch response call for exchanging corresponding responses between the provider system and the subscription system. Requests are grouped according to an extensible markup language schema and responses are grouped according to another extensible markup language schema.

Abstract: A fast method of creating nanostructures comprising the steps of forming one or more electrically-charged regions (5) of predetermined shape on a surface (1) of a first material, by contacting the regions with a stamp for transferring electric charge, and providing electrically charged nanoparticles (7) of a second material, and permitting the particles to flow in the vicinity of the regions, to be deposited on the regions.