Abstract: Embodiments of the invention include a system and method of using biomarkers in the diagnosis of disease, for example, breast cancer. A subject can be screened for breast cancer based on altered expression of one or more mRNAs in blood, plasma or salvia. Embodiments include 26 specific mRNAs for use as biomarkers to screen or distinguish healthy individuals from individuals affected with breast cancer. Levels of more than one of the mRNAs can be used to create a biomarker fingerprint for early screening of a breast cancer. Embodiments also include a kit for screening healthy subjects from subjects affected with breast cancer.

Abstract: Embodiments include a system and method of using biomarkers in the diagnosis of liver disease. A subject can be screened based on expression of specific mRNAs, miRNAs, proteins or peptides in blood, serum or plasma. Specific mRNAs/miRNAs are used as biomarkers to distinguish healthy individuals from individuals affected with a liver disease. Embodiments include 14 mRNA biomarkers to diagnose NAFL vs. healthy liver (i.e. early detection of liver disease). Embodiments also include 9 mRNA biomarkers to diagnose NAFL vs. NASH (i.e. stage of liver disease progression) and 37 mRNA biomarkers to diagnose NASH. Further embodiments include 32 miRNA biomarkers to diagnose and distinguish between NASH, hepatitis B and hepatitis C. Levels of more than one of the mRNAs, miRNAs or proteins can be scored and compared to one or more threshold values to diagnose or determine the prognosis of a liver disease. Embodiments also include a kit for screening healthy subjects from subjects affected with a liver disease.

Abstract: Embodiments of the invention include a system and method of using biomarkers in the diagnosis of lung cancer. A subject can be screened for lung cancer based on expression of specific mRNAs, miRNAs or the detection of a nucleic acid, protein, peptide or other biological molecule in blood, serum or plasma. Embodiments include 29 specific miRNAs for use as biomarkers to screen or distinguish healthy individuals from individuals affected with the disease, including a lung cancer, along with 81 additional nucleic acids, proteins or peptides for use as biomarkers to screen or distinguish healthy individuals from individuals affected with the disease, including a lung cancer. Levels of more than one of the mRNAs, miRNAs or proteins can be scored and compared to one or more threshold values to diagnose or determine the prognosis of lung cancer. Embodiments also include a kit for screening healthy subjects from subjects affected with the disease.

Abstract: Embodiments of the invention include a system and method of using biomarkers in the diagnosis of lung cancer. A subject can be screened for lung cancer based on expression of specific mRNAs, miRNAs or the detection of a nucleic acid, protein, peptide or other biological molecule in blood, serum or plasma. Embodiments include 29 specific miRNAs for use as biomarkers to screen or distinguish healthy individuals from individuals affected with the disease, including a lung cancer, along with 81 additional nucleic acids, proteins or peptides for use as biomarkers to screen or distinguish healthy individuals from individuals affected with the disease, including a lung cancer. Levels of more than one of the mRNAs, miRNAs or proteins can be scored and compared to one or more threshold values to diagnose or determine the prognosis of lung cancer. Embodiments also include a kit for screening healthy subjects from subjects affected with the disease.

Abstract: Embodiments of the invention include a system and method of using biomarkers in the diagnosis of lung cancer. A subject can be screened for lung cancer based on expression of specific mRNAs, miRNAs or the detection of a nucleic acid, protein, peptide or other biological molecule in blood, serum or plasma. Embodiments include 29 specific miRNAs for use as biomarkers to screen or distinguish healthy individuals from individuals affected with the disease, including a lung cancer, along with 81 additional nucleic acids, proteins or peptides for use as biomarkers to screen or distinguish healthy individuals from individuals affected with the disease, including a lung cancer. Levels of more than one of the mRNAs, miRNAs or proteins can be scored and compared to one or more threshold values to diagnose or determine the prognosis of lung cancer. Embodiments also include a kit for screening healthy subjects from subjects affected with the disease.