Abstract: Methods for recovery of recyclable water and/or fermentation co-products from thin stillage process streams. Microbial metabolites plant derivatives and/or plant extractives are removed from thin stillage after which water is recovered and recycled. Thin stillage is commingled with polar organic solvents or with oil to react microbial metabolites, plant derivatives and/or plant extractives. The reacted organic solvents are then separated from the processed thin stillage. Thin stillage may be commingled with an oil to re act with certain organic compounds, after which, the reacted oil is separated from the processed thin stillage is further extracted with one or more polar organic solvents. Alternatively, thin stillage may be first processed with one or more polar organic solvents, and then processed with an oil. Useful organic compounds may be recovered from the reacted oil and reacted organic solvents.

Abstract: A process for the extraction of macromolecules from a biomass material comprising: a) contacting the biomass material with a solution comprising thin stillage to provide a slurry comprising undissolved solids, dissolved solids and suspended solids; and b) separating undissolved solids from the slurry to provide a solid fraction and a liquid fraction; and wherein the macromolecules are comprised in the dissolved solids.

Abstract: Methods for separation and recovery of individual cyclic peptides from plant materials, said method comprising the steps of: (a) extracting an oil from a plant material; (b) separating the extracted oil into a non-polar fraction and a polar fraction; (c) separation and recovery of cyclic peptides from the non-polar fraction, and (d) separation and recovery of cyclic peptides from the polar fraction. The methods are suitable for separation and recovery of individual cyclolinopeptides from flax seed oil. Individual cyclolinopeptides are useful for modulation physiological disorders associated with apoptosis. Modified flaxseed oils may be produced by commingling flaxseed oils absent cyclolinopeptides, with at least one cyclolinopeptide separated and recovered with the methods disclosed herein.

Abstract: A process for the extraction of macromolecules from a biomass material comprising: a) contacting the biomass material with a solution comprising thin stillage to provide a slurry comprising undissolved solids, dissolved solids and suspended solids; and b) separating undissolved solids from the slurry to provide a solid fraction and a liquid fraction; and wherein the macromolecules are comprised in the dissolved solids

Abstract: Methods for recovery of recyclable water and/or fermentation co-products from thin stillage process streams. Microbial metabolites and/or plant derivatives and/or plant extractives are removed from thin stillage after which, recyclable water is recovered from the processed thin stillage. Thin stillage is commingled with one or more polar organic solvents to react microbial metabolites and/or plant derivatives and/or plant extractives with the organic solvents. The reacted organic solvents are then separated from the processed thin stillage. Thin stillage may be commingled with an oil to re act with certain organic compounds, after which, the reacted oil is separated from the processed thin stillage is further extracted with one or more polar organic solvents. Alternatively, thin stillage may be first processed with one or more polar organic solvents, and then processed with an oil. Useful organic compounds may recovered from the reacted oil and from the reacted organic solvents.

Abstract: Methods for separation and recovery of individual cyclic peptides from plant materials, said method comprising the steps of: (a) extracting an oil from a plant material; (b) separating the extracted oil into a non-polar fraction and a polar fraction; (c) separation and recovery of cyclic peptides from the non-polar fraction, and (d) separation and recovery of cyclic peptides from the polar fraction. The methods are suitable for separation and recovery of individual cyclolinopeptides from flax seed oil. Individual cyclolinopeptides are useful for modulation physiological disorders associated with apoptosis. Modified flaxseed oils may be produced by comingling flaxseed oils absent cyclolinopeptides, with at least one cyclolinopeptide separated and recovered with the methods disclosed herein.

Abstract: Methods for recovery of concentrates of lubricating compounds from vegetable and animal oils, fats and greases that allow separation of triglycerides, from components with higher lubricity or enrichment protocols that increase the concentration of high lubricity compounds in the triglyceride. The triglycerides are transesterified with a lower alcohol to produce alkyl esters. Following the conversion process the esters are separated from high molecular weight high lubricity compounds by distillation. The esters have some lubricity and may be sold as pollution reducing fuel components. The high boiling point compounds that are the residues of distillation, however, can contribute significant lubricity and may be used widely in lubricant applications or added to petroleum fuels to decrease friction.

Abstract: Methods for recovery of concentrates of lubricating compounds and biologically active compounds from vegetable and animal oils, fats and greases that allow separation of triglycerides, from components with higher lubricity or biological activity or enrichment protocols that increase the concentration of high lubricity or biologically active compounds in the triglyceride. The triglycerides are transesterified with a lower alcohol to produce alkyl esters. Following the conversion process the esters are separated from high molecular weight high lubricity compounds and biologically active compounds by distillation. The esters have some lubricity and may be sold as pollution reducing fuel components.

Abstract: Methods for preparation of a unique superbase catalyst consisting of mixture of polyether alcohol and base in which a polyether alcohol superbase is produced by the removal of water or alcohol at elevated temperatures to form a polyether alcohol alkoxide. The superbase catalyst is useful in, but not limited to, quantitative isomerization of alkyl esters of vegetable oils containing interrupted double bond systems to yield esters with conjugated double bond systems, alkylations, arylations, acylations, aminations, condensations, eliminations, isomerizations, rearrangements, and Wittig reactions.

Abstract: Methods for simultaneous saponification and quantitative isomerization of glyceride oils containing interrupted double bond systems, with alkali in water to yield soaps with conjugated double bond systems are disclosed. Novel methods of hydrolysis of the soap product with acid to form fatty acid-water emulsions and the breaking of those emulsions are also disclosed. The preferred embodiment uses a vegetable oil rich in linoleic acid such as sunflower or safflower oil, potassium hydroxide, phosphoric acid to neutralize the soaps, and an emulsion breaking compound which can include ethanol or other monohydric alcohol, tannins (either hydrolysable or condensed tannin) or polyethylene glycol. The reaction forms positional and geometric isomers of conjugated linoleic acid and the preferred isomer mixture is controlled by a combination of agitation, precisely controlled heating and rapid initiation and termination of the reaction. The reaction product composition may be enriched by crystallization.

Abstract: Methods for acidification of soapstock and recovery of acidulated fatty acids are disclosed. Further methods of conversion of the acidulated fatty acids to esters are also disclosed. Further methods of crystallizing the acidulated fatty acids from the extraction solvent at low temperatures and fractionating the acidulated fatty acids from the extraction solvent using urea are also disclosed. The novel method of adding both a monohydric alcohol to the soapstock to lower viscosity and a strong acid to hydrolyse the soaps prevents the formation of emulsions of soaps and oils. The preferred embodiment uses a soapstock such as soybean soapstock, sulphuric acid and isopropanol. The novel process allows the quantitative recovery of fatty acids and glyceride from the soapstock by removal of the alcohol by evaporation. Alternatively the fatty acids and glyceride may be converted to esters of monohydric alcohols.