Abstract: Disclosed are compositions and methods for studying a Tat gene. Specifically, the disclosure provides a vector comprising a double-stranded nucleic acid construct which comprises a Tat gene and a green fluorescent protein (GFP) reporter element, and further wherein the double-stranded nucleic acid construct comprising AAVS1 (adeno-associated virus integration site, a safe harbor locus) arms that flank on both sides of the Tat gene and the reporter element for integration at the human AAVS1 site by homologus recombination. Further provided are methods of using a cell comprising the vector for studying the effects of exogenous conditions on expression of the Tat gene.

Abstract: The invention of the present disclosure relates to methods of analyzing results of a high-throughput assay system for molecular functions and cell processes, comprising generation of a plasmid cDNA library for a gene of interest, conversion of the plasmid cDNA library into a lentiviral library, engineering of a cell line to encode a reporter assay to measure a specific molecular function or cell process, providing a population of cells, each cell comprising a gene of interest and an engineered reporter element, sorting the population of cells into a plurality of pools, performing targeted next-generation sequencing for the integrated gene cDNA and barcode to generate a plurality of paired end reads for each cell-sorted pool, and performing a bioinformatics analysis to determine the affect each mutation in the protein of interest has on its measured function.

Abstract: Disclosed herein, are compositions and methods for the treatment of human immunodeficiency virus infection. The compositions comprise engineered transcription activator like effector nucleases (TALENs) comprising a TALE DNA binding domain flanked by two spacer sequences, and a Fokl nuclease catalytic domain. Also, described herein, are methods of using TALENs to cleave nucleic acids; and methods of administering the TALENs to subjects at risk for or having an HIV infection.

Abstract: Disclosed are compositions and methods for studying a Tat gene. Specifically, the disclosure provides a vector comprising a double-stranded nucleic acid construct which comprises a Tat gene and a green fluorescent protein (GFP) reporter element, and further wherein the double-stranded nucleic acid construct comprising AAVS1 (adeno-associated virus integration site, a safe harbor locus) arms that flank on both sides of the Tat gene and the reporter element for integration at the human AAVS1 site by homologus recombination. Further provided are methods of using a cell comprising the vector for studying the effects of exogenous conditions on expression of the Tat gene.

Abstract: Disclosed herein, are compositions and methods for the treatment of human immunodeficiency virus infection. The compositions comprise engineered transcription activator like effector nucleases (TALENs) comprising a TALE DNA binding domain flanked by two spacer sequences, and a Fold nuclease catalytic domain. Also, described herein, are methods of using TALENs to cleave nucleic acids; and methods of administering the TALENs to subjects at risk for or having an HIV infection.

Abstract: Disclosed herein are novel compositions and methods for elucidating biological activity and detection of molecular function. The methods and compositions disclosed herein can comprise the use of one or more minimotifs and a minimotif database for integrating and coordinating orthogonal knowledge derived from a variety of technological endeavors to provide systemic models representing complex biological and molecular interactions ranging from individual cells to entire organisms. The methods and compositions disclosed herein can utilize information related to biometrics including protein/protein interaction, and gene/gene interaction for evaluating cellular functions and cellular mechanisms.