Patents by Inventor Martin Schatte
Martin Schatte has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Patent number: 11572552Abstract: Reported herein is a sortase comprising an amino acid sequence that is at least 90% identical to the amino acid sequence of SEQ ID NO: 11 and that comprises the mutations D101S and K137S.Type: GrantFiled: December 18, 2020Date of Patent: February 7, 2023Assignee: HOFFMANN-LA ROCHE INC.Inventors: Mara Boenitz-Dulat, Martin Schatte
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Patent number: 11306302Abstract: Herein is reported a polypeptide comprising the amino acid sequence of SEQ ID NO: 38 as sole Listeria monocytogenes derived polypeptide and its use in conjugating polypeptides.Type: GrantFiled: March 23, 2018Date of Patent: April 19, 2022Assignee: Hoffmann-La Roche Inc.Inventors: Mara Boenitz-Dulat, Martin Schatte
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Patent number: 11174502Abstract: Herein is reported a method for the enzymatic production of a polypeptide comprising the step of incubating i) a first polypeptide comprising the amino acid sequence LPXTG (SEQ ID NO: 01, wherein X can be any amino acid residue) or LPXTA (SEQ ID NO: 41, wherein X can be any amino acid residue), ii) a second polypeptide that has i) a glycinyl, an alaninyl, or a cysteinyl compound at its N-terminus, or ii) an oligoglycine, or oligoalanine, or a cysteine amino acid residue followed by one to three glycine or alanine amino acid residues at its N-terminus, or iii) a lysine amino acid residue within its 5 N-terminal amino acid residues, and iii) a third polypeptide with sortase A activity, in a deep eutectic solvent and thereby producing a polypeptide.Type: GrantFiled: March 23, 2018Date of Patent: November 16, 2021Assignee: Hoffmann-La Roche Inc.Inventors: Martin Schatte, Mara Boenitz-Dulat
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Patent number: 11169146Abstract: Herein is reported a method for the detection of a sortase in a sample, comprising the following steps: a) incubating the sample with a first substrate comprising an immobilization tag and a second substrate comprising a detectable label, whereby in the presence of a sortase in the sample a conjugate comprising the immobilization tag and the detectable label is formed, b) immobilizing the conjugate of step a) via/using the immobilization tag to a solid phase, c) detecting the immobilized conjugate via/using the detectable label and thereby detecting the sortase in the sample.Type: GrantFiled: June 16, 2017Date of Patent: November 9, 2021Assignee: Hoffmann-La Roche Inc.Inventors: Mara Boenitz-Dulat, Erhard Kopetzki, Peter Kratzsch, Martin Schatte
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Patent number: 11162088Abstract: Herein is reported a method for the enzymatic production of a thioester comprising incubating i) a first polypeptide comprising the amino acid sequence LPXTG (SEQ ID NO: 01, wherein X can be any amino acid residue), ii) a second polypeptide that has at its N-terminus a cysteine amino acid residue or is a cysteinyl compound, and iii) a third polypeptide with sortase A activity.Type: GrantFiled: March 23, 2018Date of Patent: November 2, 2021Assignee: Hoffmann-La Roche Inc.Inventors: Martin Schatte, Mara Boenitz-Dulat, Frank Bergmann
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Publication number: 20210317427Abstract: The present invention refers to an optimized 3-phytase which is a bio-efficacious phytase mutant having sequence identity of at least 95% around 3-phytase (SEQ ID NO. 1) comprising a sequence of SEQ ID NO. 2 and one or more mutations. The invention is further directed to the use of such optimized 3-phytase in the production of a food or feed product.Type: ApplicationFiled: July 17, 2019Publication date: October 14, 2021Inventors: Helge JOCHENS, Frank WALLRAPP, Michael HOESL, Thomas EISELE, Lutz ROECHER, Gabi GEBHARDT-WEIDL, Martin GRAEBER, Werner STUERMER, Jörg CLAREN, Timothy O'CONNELL, Martin SCHATTE, Christoph REISINGER
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Publication number: 20210238571Abstract: Reported herein is a sortase comprising an amino acid sequence that is at least 90% identical to the amino acid sequence of SEQ ID NO: 11 and that comprises the mutations D101S and K137S.Type: ApplicationFiled: December 18, 2020Publication date: August 5, 2021Applicant: Hoffmann-La Roche Inc.Inventors: Mara Boenitz-Dulat, Martin Schatte
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Patent number: 10900026Abstract: Reported herein is a sortase comprising an amino acid sequence that is at least 90% identical to the amino acid sequence of SEQ ID NO: 11 and that comprises the mutations D101S and K137S.Type: GrantFiled: September 27, 2018Date of Patent: January 26, 2021Assignee: Hoffmann-La Roche Inc.Inventors: Mara Boenitz-Dulat, Martin Schatte
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Publication number: 20200190553Abstract: A deep eutectic solvent consisting of (2-hydroxyethyl) trimethyl ammonium chloride and dithiothreitol in a molar ratio of from 1:2 to 1:3 and from 0% to 10% co-solvent, and methods of enzymatic production of polypeptides using the deep eutectic solvent.Type: ApplicationFiled: February 13, 2020Publication date: June 18, 2020Applicant: Hoffmann-La Roche Inc.Inventors: Mara Boenitz-Dulat, Martin Schatte
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Publication number: 20190085311Abstract: Reported herein is a sortase comprising an amino acid sequence that is at least 90% identical to the amino acid sequence of SEQ ID NO: 11 and that comprises the mutations D101S and K137S.Type: ApplicationFiled: September 27, 2018Publication date: March 21, 2019Applicant: Hoffmann-La Roche Inc.Inventors: Mara Boenitz-Dulat, Martin Schatte
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Publication number: 20190040436Abstract: Herein is reported a method for the enzymatic production of a polypeptide comprising the step of incubating i) a first polypeptide comprising the amino acid sequence LPXTG (SEQ ID NO: 01, wherein X can be any amino acid residue) or LPXTA (SEQ ID NO: 41, wherein X can be any amino acid residue), ii) a second polypeptide that has i) a glycinyl, an alaninyl, or a cysteinyl compound at its N-terminus, or ii) an oligoglycine, or oligoalanine, or a cysteine amino acid residue followed by one to three glycine or alanine amino acid residues at its N-terminus, or iii) a lysine amino acid residue within its 5 N-terminal amino acid residues, and iii) a third polypeptide with sortase A activity, in a deep eutectic solvent and thereby producing a polypeptide.Type: ApplicationFiled: March 23, 2018Publication date: February 7, 2019Applicant: Hoffmann-La Roche Inc.Inventors: Martin Schatte, Mara Boenitz-Dulat
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Patent number: 10190182Abstract: Herein is reported a method for producing an enzymatic conjugation product of two polypeptides comprising incubating of a first polypeptide comprising the amino acid sequence LPXTG (SEQ ID NO: 20, wherein X can be any amino acid residue), a second polypeptide has an oligo-alanine Am (m=2 (SEQ ID NO: 26), or 3 (SEQ ID NO: 27), or 4 (SEQ ID NO: 28), or 5 (SEQ ID NO: 29)) amino acid sequence at its N-terminus, a third polypeptide with sortase activity which is derived from Staphylococcus aureus Sortase A, and recovering the conjugate from the reaction mixture and thereby producing the enzymatic conjugation product of two polypeptides.Type: GrantFiled: June 16, 2017Date of Patent: January 29, 2019Assignee: HOFFMANN LA-ROCHE INC.Inventors: Mara Boenitz-Dulat, Peter Kratzsch, Martin Schatte
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Publication number: 20180334661Abstract: Herein is reported a method for the enzymatic production of a thioester comprising incubating i) a first polypeptide comprising the amino acid sequence LPXTG (SEQ ID NO: 01, wherein X can be any amino acid residue), ii) a second polypeptide that has at its N-terminus a cysteine amino acid residue or is a cysteinyl compound, and iii) a third polypeptide with sortase A activity.Type: ApplicationFiled: March 23, 2018Publication date: November 22, 2018Applicant: Hoffmann-La Roche Inc.Inventors: Martin Schatte, Mara Boenitz-Dulat, Frank Bergmann
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Publication number: 20180216091Abstract: Herein is reported a polypeptide comprising the amino acid sequence of SEQ ID NO: 38 as sole Listeria monocytogenes derived polypeptide and its use in conjugating polypeptides.Type: ApplicationFiled: March 23, 2018Publication date: August 2, 2018Applicant: Hoffmann-La Roche Inc.Inventors: Mara Boenitz-Dulat, Martin Schatte
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Publication number: 20170370913Abstract: Herein is reported a method for the detection of a sortase in a sample, comprising the following steps: a) incubating the sample with a first substrate comprising an immobilization tag and a second substrate comprising a detectable label, whereby in the presence of a sortase in the sample a conjugate comprising the immobilization tag and the detectable label is formed, b) immobilizing the conjugate of step a) via/using the immobilization tag to a solid phase, c) detecting the immobilized conjugate via/using the detectable label and thereby detecting the sortase in the sample.Type: ApplicationFiled: June 16, 2017Publication date: December 28, 2017Applicant: Hoffmann-La Roche Inc.Inventors: Mara Boenitz-Dulat, Erhard Kopetzki, Peter Kratzsch, Martin Schatte
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Publication number: 20170292166Abstract: Herein is reported a method for producing an enzymatic conjugation product of two polypeptides comprising incubating of a first polypeptide comprising the amino acid sequence LPXTG (SEQ ID NO: 20, wherein X can be any amino acid residue), a second polypeptide has an oligo-alanine Am (m=2 (SEQ ID NO: 26), or 3 (SEQ ID NO: 27), or 4 (SEQ ID NO: 28), or 5 (SEQ ID NO: 29)) amino acid sequence at its N-terminus, a third polypeptide with sortase activity which is derived from Staphylococcus aureus Sortase A, and recovering the conjugate from the reaction mixture and thereby producing the enzymatic conjugation product of two polypeptides.Type: ApplicationFiled: June 16, 2017Publication date: October 12, 2017Applicant: Hoffmann-La Roche Inc.Inventors: Mara Boenitz-Dulat, Peter Kratzsch, Martin Schatte
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Publication number: 20170226555Abstract: A deep eutectic solvent consisting of (2-hydroxyethyl) trimethyl ammonium chloride and dithiothreitol in a molar ratio of from 1:2 to 1:3 and from 0% to 10% co-solvent, and methods of enzymatic production of polypeptides using the deep eutectic solvent.Type: ApplicationFiled: February 3, 2017Publication date: August 10, 2017Applicant: Hoffmann-La Roche Inc.Inventors: Mara Boenitz-Dulat, Martin Schatte