Abstract: A biopsy needle assembly that is an improvement over the Jamshidi biopsy needle assembly is provided. The improved biopsy needle assembly includes a cannula with a uniform diameter internal surface defining an inner biopsy tissue sample receiving and retaining bore having projections thereon, and a stylet having rounded edges with at least one V-shaped groove at a distal cutting tip removably received within the bore of the cannula. The projections may be barbs, annular or spiral ribs that line the inner biopsy tissue sample receiving and retaining bore. The stylet may include a quick release knob for engaging with an improved coupling at the proximal end of the cannula. The improved coupling is substantially cylindrical with a cross handle substantially in the middle thereof. The coupling may also include internal threads engaging a syringe. The improved biopsy needle assembly permits more consistent acquisition of an adequate tissue sample for biopsy and is less painful to the patient.