Abstract: The present invention relates to an assay apparatus for detecting a product in a sample. The assay apparatus comprises a fluid pathway with an input of the sample and an input for a reagent with a detectable moiety which transports the sample to a detecting means via a barrier arranged to prevent a product-reagent complex passage but allow passage of unbound reagent and sample. The apparatus also includes supply means for supplying a substance adapted to breakdown the complex into a detectable moiety which can flow through the barrier to the detecting means.

Abstract: An apparatus and its use for chemical synthesis is disclosed. In one embodiment, the apparatus comprises a solid support input and a plurality of reagent inputs, wherein each input is connectable to a main fluid pathway via a respective switchable valve. The apparatus further comprises a reaction chamber that is fluidly connectable to the main fluid pathway by a respective switchable valve downstream of the inputs, and that includes an outlet downstream of a barrier that prevents passage of solid support but allows passage of unbound molecules.

Abstract: The present invention relates to a composition for use in fluorometry, a method of fluorometry and the use of a class of new reagents in fluorometry. The composition comprises a substrate which is a non-fluorescent derivative of a fluorophore, and a shifting reagent which shifts the absorbance wavelength maximum of the fluorophore which maximum is naturally above 450 nm. The shifting reagent is present in an amount predetermined to shift the maximum to a preset value.

Abstract: The present invention relates to an assay apparatus for detecting a product in a sample. The assay apparatus comprises a fluid pathway with an input for the sample and an input for a reagent with a detectable moiety which transports the sample to a detecting means via a barrier arranged to prevent a product-reagent complex passage but allow passage to the reagent and the sample. The complex is formed through interaction of the reagent and any of the product in the sample. The apparatus also including supply means for supplying a substance adapted to breakdown the complex into a detectable moiety which can flow through the barrier to the detecting means.