Abstract: A disposable bioassay diagnostic cartridge for performing enzyme-linked immunosorbent assay for a single patient in whole blood and plasma where the cartridge has a plurality of wells. The cartridge includes a pipette tip with internal sidewalls disposed in one well of the plurality of wells, the internal sidewalls being pretreated and coated with antibodies, a reagent mixture disposed in another well of the plurality of wells, the reagent mixture comprising at least one reagent selected from the group consisting of pH stabilizer, ionic catalyst control agent, ionic intracellular inhibitory control agent, detergent, negative control agent, chelating agent, and biocide preservative, antibody, and conjugate stabilizing agent. At least a chromogenic substrate and visualizing reagent disposed in an integrated cuvette of the plurality of wells. It also includes the detection of the hemoglobin amount in the blood sample so a correction for plasma amount in a sample can be performed.

Abstract: This invention relates to a method and apparatus for determining the activity of coagulation factors in dilute capillary whole blood, citrated whole blood and citrated plasma. It also includes the detection of the hemoglobin amount in a whole blood sample so a correction of the clotting time can be performed thereby making the clotting time values independent of hemoglobin and hematocrit effect.

Abstract: Methods for determining and/or quantifying one or more analytes in fluids are generally provided. In some embodiments, a method comprises introducing or exposing a plurality of conjugated capture structures and a plurality of metal-containing (e.g., silver) conjugated particles to a fluid comprising the analyte such that the analyte binds with both a capture structure and a metal-containing (e.g., silver) particle to form a bound complex. The bound complex may then be subjected to conditions (e.g., electrochemical conditions) that allow quantification of the analyte based on the amount of metal-containing particles present. The methods described herein may be useful for determining and quantifying relatively low concentrations of analytes present in a patient sample (e.g., a droplet of whole blood).