Patents by Inventor Martine SPAANS
Martine SPAANS has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Patent number: 10450554Abstract: The present invention relates to a polypeptide having proline-specific endoprotease activity, selected from the group consisting of i. a polypeptide comprising a mature polypeptide sequence of SEQ ID NO: 2; ii. a polypeptide that has least 70%, 75%, 80%, 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98% or 99% identity to the mature polypeptide sequence of SEQ ID NO: 2; iii. a polypeptide encoded by a nucleic acid that hybridizes under medium stringency, preferably under high stringency conditions to the complementary strand of the mature polypeptide coding sequence of SEQ ID NO:1; iv. a polypeptide encoded by a nucleic acid that has at least 70%, 75% 80%, 85%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99% or 100% identity to the mature polypeptide coding sequence of SEQ ID NO: 1. The invention further relates to a method of producing the polypeptide and a process for the preparation of a food or feed product wherein the polypeptide is used.Type: GrantFiled: May 19, 2015Date of Patent: October 22, 2019Assignee: DSM IP ASSETS B.V.Inventors: Jan Metske Van Der Laan, Peter Jozef Ida Van De Vondervoort, Chantal Christis, Martine Spaans, Angela De Bruine-Paulus, Johanna Henrica Gerdina Maria Mutsaers
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Patent number: 10294467Abstract: The present invention relates to a polypeptide having proline-specific endoprotease activity, wherein the polypeptide has less than 70% residual activity when the polypeptide has been kept at a temperature of 65° C. for 15 min. The invention further relates to a polypeptide having proline-specific endoprotease activity comprising an amino acid sequence according to SEQ ID NO: 1, wherein SEQ ID NO: 1 comprises at least one amino acid substitution selected from the group consisting of P469A, P469C, P469D, P469E, P469F, P469G, P469H, P469I, P469K, P469L, P469M, P469N, P469Q, P469R, P469S, P469T, P469V, P469W, P469Y, a nucleic acid encoding a polypeptide having proline-specific endoprotease activity, a method of making a variant polypeptide having proline-specific endoprotease activity, a recombinant host cell and a method of producing the polypeptide and a process for the preparation of a food or feed product wherein the polypeptide is used.Type: GrantFiled: June 3, 2015Date of Patent: May 21, 2019Assignee: DSM IP ASSETS B.V.Inventors: Jan Metske Van Der Laan, Peter Jozef Ida Van De Vondervoort, Chantal Christis, Martine Spaans, Angela De Bruine-Paulus
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Patent number: 10287592Abstract: The invention provides Bacillus host cell deficient in the production of a neutral protease and of a alkaline protease which host cell comprises a nucleic acid construct, wherein the nucleic acid construct comprises a polynucleotide encoding a compound of interest or encoding a compound involved in the synthesis of a compound of interest operably linked to a promoter sequence allowing for expression of the polynucleotide in the host cell, wherein the promoter comprises a bacteriophage promoter sequence, more preferably a bacteriophage SPO1 promoter sequence. Said host cell can advantageously be used in a method of production of a compound of interest.Type: GrantFiled: February 6, 2015Date of Patent: May 14, 2019Assignee: DSM IP Assets B.V.Inventors: Evert Tjeerd Van Rij, Martine Spaans, Hendrikus Bernardus Carolus Hillebrand
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Patent number: 10202594Abstract: The present invention relates to a polypeptide having proline-specific endoprotease activity, wherein the polypeptide has less than 70% residual activity when the polypeptide has been kept at a temperature of 65° C. for 15 min.Type: GrantFiled: June 3, 2015Date of Patent: February 12, 2019Assignee: DSM IP ASSETS B.V.Inventors: Jan Metske Van Der Laan, Peter Jozef Ida Van De Vondervoort, Chantal Christis, Martine Spaans, Angela De Bruine-Paulus
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Publication number: 20180119127Abstract: The present invention relates to a polypeptide having proline-specific endoprotease activity, wherein the polypeptide has less than 70% residual activity when the polypeptide has been kept at a temperature of 65° C. for 15 min.Type: ApplicationFiled: June 3, 2015Publication date: May 3, 2018Inventors: Jan Metske VAN DER LAAN, Peter Jozef Ida VAN DE VONDERVOORT, Chantal CHRISTIS, Martine SPAANS, Angela DE BRUINE-PAULUS
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Publication number: 20170198272Abstract: The present invention relates to a polypeptide having proline-specific endoprotease activity, wherein the polypeptide has less than 70% residual activity when the polypeptide has been kept at a temperature of 65° C. for 15 min. The invention further relates to a polypeptide having proline-specific endoprotease activity comprising an amino acid sequence according to SEQ ID NO: 1, wherein SEQ ID NO: 1 comprises at least one amino acid substitution selected from the group consisting of P469A, P469C, P469D, P469E, P469F, P469G, P469H, P469I, P469K, P469L, P469M, P469N, P469Q, P469R, P469S, P469T, P469V, P469W, P469Y, a nucleic acid encoding a polypeptide having proline-specific endoprotease activity, a method of making a variant polypeptide having proline-specific endoprotease activity, a recombinant host cell and a method of producing the polypeptide and a process for the preparation of a food or feed product wherein the polypeptide is used.Type: ApplicationFiled: June 3, 2015Publication date: July 13, 2017Inventors: Jan Metske VAN DER LAAN, Peter Jozef Ida VAN DE VONDERVOORT, Chantal CHRISTIS, Martine SPAANS, Angela DE BRUINE-PAULUS
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Publication number: 20170175130Abstract: The invention provides Bacillus host cell deficient in the production of a neutral protease and of a alkaline protease which host cell comprises a nucleic acid construct, wherein the nucleic acid construct comprises a polynucleotide encoding a compound of interest or encoding a compound involved in the synthesis of a compound of interest operably linked to a promoter sequence allowing for expression of the polynucleotide in the host cell, wherein the promoter comprises a bacteriophage promoter sequence, more preferably a bacteriophage SPO1 promoter sequence. Said host cell can advantageously be used in a method of production of a compound of interest.Type: ApplicationFiled: February 6, 2015Publication date: June 22, 2017Applicant: DSM IP Assets B.V.Inventors: Evert Tjeerd VAN RIJ, Martine SPAANS, Hendrikus Bernardus Carolus HILLEBRAND
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Publication number: 20170081651Abstract: The present invention relates to a polypeptide having proline-specific endoprotease activity, selected from the group consisting of i. a polypeptide comprising a mature polypeptide sequence of SEQ ID NO: 2; ii. a polypeptide that has least 70%, 75%, 80%, 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98% or 99% identity to the mature polypeptide sequence of SEQ ID NO: 2; iii. a polypeptide encoded by a nucleic acid that hybridizes under medium stringency, preferably under high stringency conditions to the complementary strand of the mature polypeptide coding sequence of SEQ ID NO:1; iv. a polypeptide encoded by a nucleic acid that has at least 70%, 75% 80%, 85%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99% or 100% identity to the mature polypeptide coding sequence of SEQ ID NO: 1. The invention further relates to a method of producing the polypeptide and a process for the preparation of a food or feed product wherein the polypeptide is used.Type: ApplicationFiled: May 19, 2015Publication date: March 23, 2017Applicant: DSM IP ASSETS B.V.Inventors: Jan Metske VAN DER LAAN, Peter Jozef Ida VAN DE VONDERVOORT, Chantal CHRISTIS, Martine SPAANS, Angela DE BRUINE-PAULUS, Johanna Henrica Gerdina Maria MUTSAERS