Patents by Inventor Marvin P. Wickens
Marvin P. Wickens has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Patent number: 10301605Abstract: Methods, kits, and compositions of matter relating to poly(UG) polymerases are disclosed. In one embodiment, a method includes: contacting an RNA substrate with a poly(UG) polymerase; and allowing the poly(UG) polymerase to add a poly(UG) sequence to the end of the RNA substrate by retaining contact between the RNA substrate and the poly(UG) polymerase for a period of time from about 1 second to about 28 days. The poly(UG) polymerase can be Caenorhabditis elegans RDE-3.Type: GrantFiled: November 17, 2015Date of Patent: May 28, 2019Assignee: Wisconsin Alumni Research FoundationInventors: Marvin P. Wickens, Melanie A. Preston
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Patent number: 10160968Abstract: Methods, kits, and compositions of matter suitable for use in RNA Tagging are disclosed. In one embodiment, a method includes: expressing a fusion protein within the cellular environment, the fusion protein including at least part of the protein of interest and a tagging domain, the tagging domain introducing a selective tag to an RNA to which the fusion protein selectively binds, the selective tag including a selective tag sequence or a selective covalent modification; allowing the tagging domain to tag the RNA to which the protein of interest selectively binds by waiting for about 1 minute to about 28 days; and identifying the tagged RNA.Type: GrantFiled: November 19, 2015Date of Patent: December 25, 2018Assignee: Wisconsin Alumni Research FoundationInventors: Marvin P. Wickens, Christopher P. Lapointe, Melanie A. Preston
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Publication number: 20160145666Abstract: Methods, kits, and compositions of matter relating to poly(UG) polymerases are disclosed. In one embodiment, a method includes: contacting an RNA substrate with a poly(UG) polymerase; and allowing the poly(UG) polymerase to add a poly(UG) sequence to the end of the RNA substrate by retaining contact between the RNA substrate and the poly(UG) polymerase for a period of time from about 1 second to about 28 days. The poly(UG) polymerase can be Caenorhabditis elegans RDE-3.Type: ApplicationFiled: November 17, 2015Publication date: May 26, 2016Inventors: Marvin P. Wickens, Melanie A. Preston
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Publication number: 20160138012Abstract: Methods, kits, and compositions of matter suitable for use in RNA Tagging are disclosed. In one embodiment, a method includes: expressing a fusion protein within the cellular environment, the fusion protein including at least part of the protein of interest and a tagging domain, the tagging domain introducing a selective tag to an RNA to which the fusion protein selectively binds, the selective tag including a selective tag sequence or a selective covalent modification; allowing the tagging domain to tag the RNA to which the protein of interest selectively binds by waiting for about 1 minute to about 28 days; and identifying the tagged RNA.Type: ApplicationFiled: November 19, 2015Publication date: May 19, 2016Inventors: Marvin P. Wickens, Christopher P. Lapointe, Melanie A. Preston
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Publication number: 20040235001Abstract: An isolated preparation of a regulatory poly(A) polymerase (PAP), wherein the polymerase comprises both a catalytic subunit and an RNA-binding subunit is disclosed.Type: ApplicationFiled: September 18, 2003Publication date: November 25, 2004Inventors: Liaoteng Wang, Marvin P. Wickens, Judith E. Kimble
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Patent number: 6303311Abstract: A method for determining the function of a test protein is disclosed. In a preferred embodiment, this method comprises the steps of fusing a test protein to an RNA-binding protein and exposing the fusion protein to recombinant reporter mRNA molecule that comprises a binding site for the RNA-binding protein in the 3′ untranslated region. One then observes the properties of reporter mRNA and correlates the reporter properties with test protein function.Type: GrantFiled: July 27, 1999Date of Patent: October 16, 2001Assignees: Wisconsin Alumni Research Foundation, Arizona Board of Regents on behalf of the University of ArizonaInventors: Marvin P. Wickens, Roy Parker
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Patent number: 5985575Abstract: A method for determining the function of a test protein is disclosed. In a preferred embodiment, this method comprises the steps of fusing a test protein to an RNA-binding protein and exposing the fusion protein to recombinant reporter mRNA molecule that comprises a binding site for the RNA-binding protein in the 3' untranslated region. One then observes the properties of reporter mRNA and correlates the reporter properties with test protein function.Type: GrantFiled: May 20, 1998Date of Patent: November 16, 1999Assignees: Wisconsin Alumni Research Foundation, The Arizona Board of Regents on behalf of the University of ArizonaInventors: Marvin P. Wickens, Roy Parker
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Patent number: 5750667Abstract: A method for detecting an interaction between an RNA-binding protein and a test RNA molecule is disclosed. This method comprises providing a host cell containing a detectable gene. The detectable gene expresses a detectable protein when the detectable gene is activated by an amino acid sequence including a transcriptional activation domain when the transcriptional activation domain is in sufficient proximity to the detectable gene. First, second and third chimeric genes are also provided. The first chimeric gene comprises a DNA-binding domain that recognizes a binding site on the detectable gene in the host cell and a first RNA-binding domain. The second chimeric gene comprises a transcriptional activation domain and a second RNA-binding domain. The third chimeric gene comprises a first RNA sequence capable of binding to either the first or second RNA-binding and a second RNA sequence to be tested for interaction with the RNA-binding protein not bound to the first RNA sequence.Type: GrantFiled: November 12, 1996Date of Patent: May 12, 1998Assignee: Wisconsin Alumni Research FoundationInventors: Marvin P. Wickens, Stanley Fields
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Patent number: 5726059Abstract: Translation characteristics of an mRNA molecule in a cell can be altered by providing a prosthetic RNA molecule that includes a regulatory sequence element and a sequence element complementary to a portion of the mRNA molecule. Alteration can affect the translation rate, the mRNA stability, or the localization of the mRNA molecule.Type: GrantFiled: June 6, 1995Date of Patent: March 10, 1998Assignee: Wisconsin Alumni Research FoundationInventors: Marvin P. Wickens, Michael D. Sheets
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Patent number: 5677131Abstract: A method for detecting an interaction between an RNA-binding protein and a test RNA molecule is disclosed. This method comprises providing a host cell containing a detectable gene. The detectable gene expresses a detectable protein when the detectable gene is activated by an amino acid sequence including a transcriptional activation domain when the transcriptional activation domain is in sufficient proximity to the detectable gene. First, second and third chimeric genes are also provided. The first chimeric gene comprises a DNA-binding domain that recognizes a binding site on the detectable gene in the host cell and a first RNA-binding domain. The second chimeric gene comprises a transcriptional activation domain and a second RNA-binding domain. The third chimeric gene comprises a first RNA sequence capable of binding to either the first or second RNA-binding and a second RNA sequence to be tested for interaction with the RNA-binding protein not bound to the first RNA sequence.Type: GrantFiled: July 9, 1996Date of Patent: October 14, 1997Assignee: Wisconsin Alumni Research FoundationInventors: Marvin P. Wickens, Stanley Fields
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Patent number: 5610015Abstract: A method for detecting an interaction between an RNA-binding protein and a test RNA molecule is disclosed. This method comprises providing a host cell containing a detectable gene. The detectable gene expresses a detectable protein when the detectable gene is activated by an amino acid sequence including a transcriptional activation domain when the transcriptional activation domain is in sufficient proximity to the detectable gene. First, second and third chimeric genes are also provided. The first chimeric gene comprises a DNA-binding domain that recognizes a binding site on the detectable gene in the host cell and a first RNA-binding domain. The second chimeric gene comprises a transcriptional activation domain and a second RNA-binding domain. The third chimeric gene comprises a first RNA sequence capable of binding to either the first or second RNA-binding and a second RNA sequence to be tested for interaction with the RNA-binding protein not bound to the first RNA sequence.Type: GrantFiled: March 23, 1995Date of Patent: March 11, 1997Assignees: Wisconsin Alumni Research Foundation, State University of New York at Stony BrookInventors: Marvin P. Wickens, Stanley Fields