Abstract: A method is provided to simultaneously identify and quantify critical structure attributes (CSAs) in proteoforms or proteoform families that includes the steps of: 1. injecting a sample into a reagent stream of one or more detection enhanced molecular recognition reagents; 2. incubating the combined sample:reagent(s) during migration through an analytical platform the causes molecular recognition reagent(s) to be sequestered by a specific critical structure attribute (CSA) in the analyte to form a luminon (An:*Sas) complex; 3. using a sequestron selector in the reagent stream to overtake the luminon complex in a down-stream size exclusion chromatography (SEC) column to achieve mixing and formation of an Nc˜Pas:An:*Sas, Pas:An:*Sas, or *Pas:An:*Sas sequestome complex; 4. resolving this sequestome complex from any unbound affinity selector(s) such as *Sas or *Pas, and non-analytes in the SEC column; 5.

Abstract: A method is provided to simultaneously identify and quantify critical structure attributes (CSAs) in proteoforms or proteoform families that includes the steps of: 1. injecting a sample into a reagent stream of one or more detection enhanced molecular recognition reagents; 2. incubating the combined sample:reagent(s) during migration through an analytical platform the causes molecular recognition reagent(s) to be sequestered by a specific critical structure attribute (CSA) in the analyte to form a luminon (An:*Sas) complex; 3. using a sequestron selector in the reagent stream to overtake the luminon complex in a down-stream size exclusion chromatography (SEC) column to achieve mixing and formation of an Nc˜Pas:An:*Sas, Pas:An:*Sas, or *Pas:An:*Sas sequestome complex; 4. resolving this sequestome complex from any unbound affinity selector(s) such as *Sas or *Pas, and non-analytes in the SEC column; 5.

Abstract: A proteoformic analytical platform and method enables analysis of multiple function-related structural features in single recombinant therapeutic protein (RTP) proteoform, rather than in a family of proteoforms. The platform and method achieve rapid identification and quantification of biological quality attributes (BQAs) of the (RTP), and provides a therapeutic performance appraisal (TPA) that assesses the quality of RTP.

Abstract: A method is provided to simultaneously identify and quantify critical structure attributes (CSAs) in proteoforms or proteoform families that includes the steps of: 1. injecting a sample into a reagent stream of one or more detection enhanced molecular recognition reagents; 2. incubating the combined sample:reagent(s) during migration through an analytical platform the causes molecular recognition reagent(s) to be sequestered by a specific critical structure attribute (CSA) in the analyte to form a luminon (An:*Sas) complex; 3. using a sequestron selector in the reagent stream to overtake the luminon complex in a down-stream size exclusion chromatography (SEC) column to achieve mixing and formation of an Nc˜Pas:An:*Sas, Pas:An:*Sas, or *Pas:An:*Sas sequestome complex; 4. resolving this sequestome complex from any unbound affinity selector(s) such as *Sas or *Pas, and non-analytes in the SEC column; 5.