Abstract: The present invention establishes a molecular therapy for glycogen storage disease type Ia. The present invention provides an oligonucleotide of 15-30 bases comprising a nucleotide sequence complementary to die cDNA of G6PC gene with c.648G>T mutation, wherein the oligonucleotide comprises a sequence complementary to a region comprising any site between the 82nd to the 92nd nucleotide from the 5? end of exon 5 of the G6PC gene with c.648C>T mutation, a pharmacologically acceptable salt or solvate thereof. Also provided is a pharmaceutical drug comprising the oligonucleotide, a pharmacologically acceptable salt or solvate thereof (e.g., therapeutic drug for glycogen storage disease type Ia).

Abstract: This invention is intended to discover a novel solvent that can be used as an alternative to toluene in the step of deprotection in the method of solid-phase nucleic acid synthesis. With the use of such novel solvent, various problems caused by the use of toluene are dissolved. This invention is also intended to provide a method of solid-phase nucleic acid synthesis in which protected nucleoside phosphoramidites in which a protective group is bonded to a hydroxyl group at the 5?position or the 3? position of a nucleoside are sequentially bound on a solid phase carrier, where a reaction of removing the protecting group from the protected nucleoside phosphoramidite is carried out in a solution comprising an acid with a pKa of 0.2 to 0.8 and acetonitrile.

Abstract: This seal state detection device includes a plurality of part case members including a cylinder facing part and a rod facing part and are aligned in a circumferential direction of a cylinder by causing the cylinder facing part to face the cylinder and the rod facing part to face a rod so that a housing chamber for housing a part of the cylinder and a part of the rod is formed between the cylinder facing part and the rod facing part. The cylinder facing part includes a seal part which contacts with the cylinder. The seal part includes a protruding part formed at one end and a recessed part formed at the other end in the circumferential direction of the cylinder. The protruding part and the recessed part are fitted to each other when the plurality of part case members are aligned in the circumferential direction of the cylinder.

Abstract: This invention is intended to discover a novel solvent that can be used as an alternative to toluene in the step of deprotection in the method of solid-phase nucleic acid synthesis. With the use of such novel solvent, various problems caused by the use of toluene are dissolved. This invention is also intended to provide a method of solid-phase nucleic acid synthesis in which protected nucleoside phosphoramidites in which a protective group is bonded to a hydroxyl group at the 5?position or the 3? position of a nucleoside are sequentially bound on a solid phase carrier, where a reaction of removing the protecting group from the protected nucleoside phosphoramidite is carried out in a solution comprising an acid with a pKa of 0.2 to 0.8 and acetonitrile.

Abstract: A method for measuring concentration of dehydrogenase and substrate thereof in the presence of living cells, wherein an electron mediator that is hard to be reduced by living cells, for example, a compound represented by Formula (I), an electron mediator represented by the Formula (I) and a measurement reagent for dehydrogenase and substrate thereof including the electron mediator are disclosed. A-L-M??(I), wherein A represents a structural part containing at least one anionic group, M represents a structural part for mediating electron transfer and L represents a linker part for linking the A to the M.

Abstract: The invention provides a cut-out method capable of suppressing production of a byproduct, wherein the object RNA oligonucleotide is not cleaved from a universal linker, which is produced in cutting out the object RNA oligonucleotide from the universal support, and capable of increasing the yield of the object RNA oligonucleotide. The RNA oligonucleotide cut-out method includes a step of bringing a universal support supporting an RNA oligonucleotide in contact with an aqueous solution containing alkylamine and a monovalent inorganic salt.

Abstract: A method for measuring concentration of dehydrogenase and substrate thereof in the presence of living cells, wherein an electron mediator that is hard to be reduced by living cells, for example, a compound represented by Formula (I) shown below, an electron mediator represented by the Formula (I) shown below and a measurement reagent for dehydrogenase and substrate thereof comprising the electron mediator are disclosed. A?L?M ??(I) [In the Formula (I), A represents a structural part containing at least one anionic group, M represents a structural part for mediating electron transfer and L represents a linker part for linking the A of the M.

Abstract: The invention provides a cut-out method capable of suppressing production of a byproduct, wherein the object RNA oligonucleotide is not cleaved from a universal linker, which is produced in cutting out the object RNA oligonucleotide from the universal support, and capable of increasing the yield of the object RNA oligonucleotide. The RNA oligonucleotide cut-out method includes a step of bringing a universal support supporting an RNA oligonucleotide in contact with an aqueous solution containing alkylamine and a monovalent inorganic salt.

Abstract: A vehicle ID radar system has a simple construction and obtains high identifying performance by making a general identification based on a plurality of responses. There are provided an antenna for obtaining a plurality of question signals from a base station radar unit, a receiver for processing the signals received from the antenna, a question code demodulator for demodulating question codes from the received signals, a question code decoder for decoding the question codes and reading responses respectively corresponding to the plurality of questions from a plurality of data bases, a response code generator for generating response signals, and a transmitter for modulating the response signals and supplying them to the antenna.

Abstract: A signal discrimination device using a neural network for discriminating input signals such as radar reception signals includes an adaptive code generator means for generating codes for representing the discrimination categories. The distances between the codes for closely related categories are smaller than the distances between the codes for remotely related categories. During the learning stage, the neural network is trained to output the codes for respective inputs. The discrimination result judgment means determines the categories by comparing the outputs of the neural network and the codes for the respective categories.