Abstract: Disclosed is a nucleic acid amplification method which is based on a new principle and enables to amplify a nucleic acid having a specific nucleotide sequence in a simple manner, within a short time and with efficiency. The nucleic acid amplification method comprises the steps of: (a) obtaining a linear DNA fragment by performing a DNA polymerase elongation reaction by using a template DNA comprising a base sequence to be amplified and a primer pair comprising a primer having a base sequence complementary to a region adjacent to a 3? end of the base sequence to be amplified and a chemically modified 3? end; and (b) performing a strand displacement-type DNA polymerase elongation reaction on a circular single-stranded DNA comprising the base sequence to be amplified and serving as a template, with a 3? end of the linear DNA fragment obtained in (a) serving as an origin of replication.

Abstract: Microprotein-inactivating ultrafine metal particles comprising ultrafine metal particles having a bond between an organic acid component and a metal, having an infrared absorption peak ascribable to the bond between the organic acid and the metal near 1518 cm?1, and capable of efficiently inactivating microproteins without impairing properties of the resin or formability thereof. When used in the form of a resin composition or a coating agent, the microprotein-inactivating ultrafine metal particles are capable of exhibiting an effect for inactivating microproteins.

Abstract: Disclosed is a nucleic acid amplification method which is based on a new principle and enables to amplify a nucleic acid having a specific nucleotide sequence in a simple manner, within a short time and with efficiency. The nucleic acid amplification method comprises the steps of: (a) conducting a DNA polymerase chain reaction by using, as a template, DNA comprising a nucleotide sequence to be amplified and using a primer pair having a nucleotide sequence complementary to the nucleotide sequence to be amplified, thereby producing a linear DNA fragment; and (b) conducting a chain-substituting DNA polymerase chain reaction in a chaining manner by using cyclic single-stranded DNA comprising the same nucleotide sequence as that of at least one of the primer pair as a template and employing the 3?-terminus of the linear DNA fragment produced in step (a) as the replication origin.

Abstract: Microprotein-inactivating ultrafine metal particles comprising ultrafine metal particles having a bond between an organic acid component and a metal, having an infrared absorption peak ascribable to the bond between the organic acid and the metal near 1518 cm?1, and capable of efficiently inactivating microproteins without impairing properties of the resin or formability thereof. When used in the form of a resin composition or a coating agent, the microprotein-inactivating ultrafine metal particles are capable of exhibiting an effect for inactivating microproteins.

Abstract: Disclosed is a nucleic acid amplification method which is based on a new principle and enables to amplify a nucleic acid having a specific nucleotide sequence in a simple manner, within a short time and with efficiency. The nucleic acid amplification method comprises the steps of: (a) conducting a DNA polymerase chain reaction by using, as a template, DNA comprising a nucleotide sequence to be amplified and using a primer pair having a nucleotide sequence complementary to the nucleotide sequence to be amplified, thereby producing a linear DNA fragment; and (b) conducting a chain-substituting DNA polymerase chain reaction in a chaining manner by using cyclic single-stranded DNA comprising the same nucleotide sequence as that of at least one of the primer pair as a template and employing the 3?-terminus of the linear DNA fragment produced in step (a) as the replication origin.