Patents by Inventor Masaharu Yoshioka

Masaharu Yoshioka has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Promoters and utilization thereof
    Patent number: 7385048
    Abstract: The present invention relates to improved promoters and utilization thereof, in particular to promoters which are improved so as not to undergo methylation in the course of constructing transformants, and utilization thereof. According to the present invention, the expression efficiency of a structural gene can be enhanced even in a plant, e.g. chrysanthemum, which has weak expression of the structural gene by a cauliflower mosaic virus 35S promoter which as been considered a high expression promoter for plants.
    Type: Grant
    Filed: August 9, 2006
    Date of Patent: June 10, 2008
    Assignee: Kirin Beer Kabushiki Kaisha
    Inventors: Toshio Fujii, Toshiya Ogawa, Masaharu Yoshioka, Kanji Mamiya, Toshihiro Toguri
  • Promoters and utilization thereof
    Publication number: 20060277623
    Abstract: The present invention relates to improved promoters and utilization thereof, in particular to promoters which are improved so as not to undergo methylation in the course of constructing transformants, and utilization thereof. The improved promoters of the present invention are represented by the following (1) and (2): (1) a DNA comprising the nucleotide sequences shown in SEQ ID NOS: 1 to 4; (2) a DNA comprising a nucleotide sequence consisting of the nucleotide sequences shown in SEQ ID NOS 1 to 4, wherein one to several nucleotides are deleted, added or inserted and the deleted, added or inserted sequence is free from any consecutive sequences represented by CG, CAG, CTG, CCG or CGG; the DNA having promoter activity. According to the present invention, the expression efficiency of a structural gene can be enhanced even in a plant, e.g. chrysanthemum, which has weak expression of the structural gene by a cauliflower mosaic virus 35S promoter which has been considered a high expression promoter for plants.
    Type: Application
    Filed: August 9, 2006
    Publication date: December 7, 2006
    Inventors: Toshio Fujii, Toshiya Ogawa, Masaharu Yoshioka, Kanji Mamiya, Toshihiro Toguri
  • Promoters and utilization thereof
    Patent number: 7115420
    Abstract: The present invention relates to improved promoters and utilization thereof, in particular to promoters which are improved so as not to undergo methylation in the course of constructing transformants, and utilization thereof. According to the present invention, the expression efficiency of a structural gene can be enhanced even in a plant, e.g. chrysanthemum, which has weak expression of the structural gene by a cauliflower mosaic virus 35S promoter which as been considered a high expression promoter for plants.
    Type: Grant
    Filed: February 23, 2001
    Date of Patent: October 3, 2006
    Assignee: Kirin Beer Kabushiki Kaisha
    Inventors: Toshio Fujii, Toshiya Ogawa, Masaharu Yoshioka, Kanji Mamiya, Toshihiro Toguri
  • Promoters and utilization thereof
    Publication number: 20030177519
    Abstract: The present invention relates to improved promoters and utilization thereof, in particular to promoters which are improved so as not to undergo methylation in the course of constructing transformants, and utilization thereof.
    Type: Application
    Filed: September 3, 2002
    Publication date: September 18, 2003
    Inventors: Toshio Fujii, Toshiya Ogawa, Masaharu Yoshioka, Kanji Mamiya, Toshiro Toguri
  • Virus resistant plants expressing animal cell-derived (2?-5?)oligadenylate synthetase and ribonuclease L and A method for creating the same
    Patent number: 6320099
    Abstract: The present invention relates to a method for creating a plant having resistance to RNA viruses, comprising incorporating a DNA sequence encoding an animal cell-derived (2′-5′)oligoadenylate synthetase and a DNA sequence encoding an animal cell-derived ribonuclease L into a chromosome(s) of the plant and expressing the DNA sequences, as well as plants created by the above method. The present invention is widely applicable to the breeding of virus resistant plant varieties.
    Type: Grant
    Filed: December 1, 1997
    Date of Patent: November 20, 2001
    Assignee: Kirin Beer Kabushiki Kaisha
    Inventors: Toshiya Ogawa, Masaharu Yoshioka, Isao Ishida