Patents by Inventor Masahiro Kinooka
Masahiro Kinooka has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
-
Patent number: 11795438Abstract: Provided are a method for promoting the proliferation of pluripotent stem cells and a proliferation promoting composition used in the method. In one aspect, a composition for promoting the proliferation of pluripotent stem cells includes hemagglutinin or modified hemagglutinin. In another aspect, a method for promoting the proliferation of pluripotent stem cells includes culturing pluripotent stem cells in a culture medium to which the proliferation promoting composition of the present disclosure has been added.Type: GrantFiled: December 19, 2017Date of Patent: October 24, 2023Assignee: Osaka UniversityInventors: Masahiro Kinooka, Meehae Kim, Yukako Fujinaga
-
Publication number: 20230035766Abstract: The presently disclosed subject matter provides a cell culture evaluation system including a measurement unit, a first storage unit, a second storage unit, an evaluation unit and an output unit, wherein: the measurement unit includes a sensor that acquires evaluation target data about motion or a state of a culture tool; the first storage unit stores the evaluation target data; the second storage unit stores reference data; the evaluation unit compares the evaluation target data with the reference data so as to evaluate an execution state of a cell culture process; and the output unit outputs an evaluation result created by the evaluation unit.Type: ApplicationFiled: December 21, 2020Publication date: February 2, 2023Inventors: Hodaka Makino, Hirotsugu Kubo, Masahiro Kinooka
-
Patent number: 11505774Abstract: A sample storage apparatus includes: a lid member; a sample storage container; first and second fluid sucking sections; first and second fluid discharging sections; first to fourth flow paths; and a first fluid identification sensor. The first flow path is connected to the first fluid sucking section, the second flow path is connected to the second fluid sucking section, the third flow path is connected to the first fluid discharging section, and the fourth flow path is connected to the second fluid discharging section. The first fluid identification sensor for identifying a fluid is disposed in the first flow path.Type: GrantFiled: October 22, 2019Date of Patent: November 22, 2022Assignees: NIHON KOHDEN CORPORATION, OSAKA UNIVERSITYInventors: Hodaka Makino, Hirotsugu Kubo, Tetsuya Ogawa, Masahiro Kinooka
-
Publication number: 20220290106Abstract: Provided is a method that is able to simply promote cell proliferation. An aspect relates to a method for promoting proliferation of cells. The method includes adding a precursor of AGES to a culture medium containing a cell aggregate, and culturing the cell aggregate in the culture medium to which the precursor of AGEs has been added.Type: ApplicationFiled: July 10, 2020Publication date: September 15, 2022Applicant: Osaka UniversityInventors: Masahiro Kinooka, Meehae Kim
-
Patent number: 11318456Abstract: An aseptic sampling apparatus includes an isolator, a liquid delivery port that is disposed in the isolator, a sampling section that is disposed inside the isolator, a first flow path that communicates with a discharge flow path of the sampling section, and that connects an inside and outside of the isolator to each other through the liquid delivery port, a fluid supplying unit that supplies a fluid to the sampling section, a gas supplying unit that communicates with the fluid supplying unit, and a seal member that prevents the fluid supplied from fluid supplying unit to the discharge flow path from leaking.Type: GrantFiled: January 3, 2019Date of Patent: May 3, 2022Assignees: NIHON KOHDEN CORPORATION, OSAKA UNIVERSITYInventors: Hodaka Makino, Hirotsugu Kubo, Tetsuya Ogawa, Masahiro Kinooka
-
Patent number: 11320344Abstract: An aseptic sampling flow path kit to be applied to an isolator having a liquid delivery port includes a sampling section, a first flow path that communicates with the sampling section, and that connects an inside of the isolator to an outside of the isolator through the liquid delivery port, and at least one one-way valve which is disposed in the first flow path, and that limits flow of fluid in the first flow path to a direction from the sampling section toward the liquid delivery port. In the kit, at least a part of the first flow path is a germicidal flow path to which a germicidal unit is applicable.Type: GrantFiled: January 2, 2019Date of Patent: May 3, 2022Assignees: NIHON KOHDEN CORPORATION, OSAKA UNIVERSITYInventors: Hodaka Makino, Hirotsugu Kubo, Masahiro Kinooka
-
Publication number: 20220113232Abstract: Provided is a distribution method with which particle components such as cells can be dispensed. In one aspect, provided is a method for distributing particles, the method including dispersing the particles in a plastic fluid, and dispensing the plastic fluid containing the particles. In another aspect, provided is a method for producing a product including a particle and a container for the particle, the method including dispensing a plastic fluid in which the particles are dispersed, from a storage part in which the plastic fluid is stored, to a plurality of the containers.Type: ApplicationFiled: August 13, 2019Publication date: April 14, 2022Applicant: Osaka UniversityInventor: Masahiro Kinooka
-
Patent number: 11230701Abstract: Provided are a novel hemagglutinin that can remove cells deviated from the undifferentiated state, the cells being cells that emerge in a colony during culture of stem cells having pluripotency, and a novel method for culturing stem cells having pluripotency, the method using hemagglutinin. Provided is a mutant hemagglutinin complex protein derived from Clostridium botulinum type B, the complex protein containing at least subcomponents HA2 and HA3 of hemagglutinin derived from Clostridium botulinum type B, and the complex protein containing amino acids constituting a glycosylation site, with at least one of the amino acids having been mutated. Provided is a method for culturing stem cells having pluripotency, the method including culturing the stem cell having pluriopotency in the presence of a mutant hemagglutinin complex protein of the present disclosure.Type: GrantFiled: June 29, 2015Date of Patent: January 25, 2022Assignee: Osaka UniversityInventors: Masahiro Kinooka, Meehae Kim, Yukako Fujinaga, Yo Sugawara
-
Patent number: 11186810Abstract: A cell culture container includes a storage container, a cell culture area, a culture solution supply unit, and a discharge unit. The cell culture area is a region for culturing cells, and is provided in the storage container. The culture solution supply unit supplies the culture solution into the cell culture area at a flow rate equal to or less than a predetermined flow rate. The discharge unit discharges the liquid in the cell culture area to the outside of the storage container. A rectification unit is a structure in which a plurality of outflow ports are uniformly arranged in a direction perpendicular to the flow direction of the culture solution from the culture solution supply unit to the discharge unit.Type: GrantFiled: March 16, 2016Date of Patent: November 30, 2021Assignees: SHIMADZU CORPORATION, YOKOHAMA CITY UNIVERSITY, OSAKA UNIVERSITY, CORNING INCORPORATEDInventors: Tomoki Ohkubo, Masaki Kanai, Hirohisa Abe, Hideki Taniguchi, Masahiro Kinooka, Goro Kobayashi, Takanori Takebe
-
Publication number: 20210122790Abstract: The present invention provides a miniaturized hemagglutinin complex protein having function inhibitory activity against E-cadherin, wherein (a) all or a part of at least HA1 subcomponent, and/or a part of HA3 subcomponent are/is deleted, (b) regions in HA2 and HA3 subcomponents that contribute to binding with E-cadherin are present, and (c) the HA3 subcomponent is derived from Clostridium botulinum type A or type B, and an E-cadherin function inhibitor containing the hemagglutinin complex protein.Type: ApplicationFiled: November 22, 2018Publication date: April 29, 2021Applicants: NATIONAL UNIVERSITY CORPORATION KANAZAWA UNIVERSITY, OSAKA UNIVERSITYInventors: Yukako FUJINAGA, Sho AMATSU, Masahiro KINOOKA
-
Patent number: 10954487Abstract: Provided is a cell culturing method in which physical stress is reduced and oxygen can be supplied. The invention relates to a cell culturing method including; arranging cells in a dispersed state in a culture medium which is a plastic fluid; and introducing air bubbles into said culture medium.Type: GrantFiled: January 20, 2017Date of Patent: March 23, 2021Assignee: Osaka UniversityInventors: Masahiro Kinooka, Eiji Nagamori
-
Publication number: 20200123489Abstract: A sample storage apparatus includes: a lid member; a sample storage container; first and second fluid sucking sections; first and second fluid discharging sections; first to fourth flow paths; and a first fluid identification sensor. The first flow path is connected to the first fluid sucking section, the second flow path is connected to the second fluid sucking section, the third flow path is connected to the first fluid discharging section, and the fourth flow path is connected to the second fluid discharging section. The first fluid identification sensor for identifying a fluid is disposed in the first flow path.Type: ApplicationFiled: October 22, 2019Publication date: April 23, 2020Applicants: NIHON KOHDEN CORPORATION, OSAKA UNIVERSITYInventors: Hodaka MAKINO, Hirotsugu KUBO, Tetsuya OGAWA, Masahiro KINOOKA
-
Publication number: 20200017836Abstract: Provided are a method for promoting the proliferation of pluripotent stem cells and a proliferation promoting composition used in the method. In one aspect, a composition for promoting the proliferation of pluripotent stem cells includes hemagglutinin or modified hemagglutinin. In another aspect, a method for promoting the proliferation of pluripotent stem cells includes culturing pluripotent stem cells in a culture medium to which the proliferation promoting composition of the present disclosure has been added.Type: ApplicationFiled: December 19, 2017Publication date: January 16, 2020Applicant: Osaka UniversityInventors: Masahiro Kinooka, Meehae Kim, Yukako Fujinaga
-
Publication number: 20190247842Abstract: An aseptic sampling apparatus includes an isolator, a liquid delivery port that is disposed in the isolator, a sampling section that is disposed inside the isolator, a first flow path that communicates with a discharge flow path of the sampling section, and that connects an inside and outside of the isolator to each other through the liquid delivery port, a fluid supplying unit that supplies a fluid to the sampling section, a gas supplying unit that communicates with the fluid supplying unit, and a seal member that prevents the fluid supplied from fluid supplying unit to the discharge flow path from leaking.Type: ApplicationFiled: January 3, 2019Publication date: August 15, 2019Applicants: NIHON KOHDEN CORPORATION, OSAKA UNIVERSITYInventors: Hodaka MAKINO, Hirotsugu KUBO, Tetsuya OGAWA, Masahiro KINOOKA
-
Publication number: 20190250073Abstract: An aseptic sampling flow path kit to be applied to an isolator having a liquid delivery port includes a sampling section, a first flow path that communicates with the sampling section, and that connects an inside of the isolator to an outside of the isolator through the liquid delivery port, and at least one one-way valve which is disposed in the first flow path, and that limits flow of fluid in the first flow path to a direction from the sampling section toward the liquid delivery port. In the kit, at least a part of the first flow path is a germicidal flow path to which a germicidal unit is applicable.Type: ApplicationFiled: January 2, 2019Publication date: August 15, 2019Applicants: NIHON KOHDEN CORPORATION, OSAKA UNIVERSITYInventors: Hodaka MAKINO, Hirotsugu KUBO, Masahiro KINOOKA
-
Publication number: 20190078044Abstract: A cell culture container includes a storage container, a cell culture area, a culture solution supply unit, and a discharge unit. The cell culture area is a region for culturing cells, and is provided in the storage container. The culture solution supply unit supplies the culture solution into the cell culture area at a flow rate equal to or less than a predetermined flow rate. The discharge unit discharges the liquid in the cell culture area to the outside of the storage container. A rectification unit is a structure in which a plurality of outflow ports are uniformly arranged in a direction perpendicular to the flow direction of the culture solution from the culture solution supply unit to the discharge unit.Type: ApplicationFiled: March 16, 2016Publication date: March 14, 2019Applicants: Shimadzu Corporation, Yokohama City University, Osaka University, Kuraray Co., Ltd.Inventors: Tomoki OHKUBO, Masaki KANAI, Hirohisa ABE, Hideki TANIGUCHI, Masahiro KINOOKA, Goro KOBAYASHI, Takanori TAKEBE
-
Publication number: 20190032006Abstract: Provided is a cell culturing method in which physical stress is reduced and oxygen can be supplied. The invention relates to a cell culturing method including; arranging cells in a dispersed state in a culture medium which is a plastic fluid; and introducing air bubbles into said culture medium.Type: ApplicationFiled: January 20, 2017Publication date: January 31, 2019Applicant: Osaka UniversityInventors: Masahiro Kinooka, Eiji Nagamori
-
Patent number: 9822344Abstract: Provided is a method by which cells deviated from the undifferentiated state, which emerge in a colony during culture of stem cells having pluripotency, can be removed. In one aspect, provided is a method for culturing stem cells having pluripotency, the method including performing cell culture in the presence of a substance that can inhibit cell-cell adhesion. In another aspect, provided is a method for removing cells deviated from the undifferentiated state, the cells being cells that have emerged or may possibly emerge during culture of stem cells having pluripotency, the method including performing cell culture in the presence of a substance that can inhibit cell-cell adhesion. In still another aspect, provided is a method for maintaining the undifferentiated state of stem cells having pluripotency, the method including performing cell culture in the presence of a substance that can inhibit cell-cell adhesion.Type: GrantFiled: December 26, 2013Date of Patent: November 21, 2017Assignee: Osaka UniversityInventors: Masahiro Kinooka, Meehae Kim, Yukako Fujinaga, Yo Sugawara
-
Publication number: 20170130206Abstract: Provided are a novel hemagglutinin that can remove cells deviated from the undifferentiated state, the cells being cells that emerge in a colony during culture of stem cells having pluripotency, and a novel method for culturing stem cells having pluripotency, the method using hemagglutinin. Provided is a mutant hemagglutinin complex protein derived from Clostridium botulinum type B, the complex protein containing at least subcomponents HA2 and HA3 of hemagglutinin derived from Clostridium botulinum type B, and the complex protein containing amino acids constituting a glycosylation site, with at least one of the amino acids having been mutated. Provided is a method for culturing stem cells having pluripotency, the method including culturing the stem cell having pluriopotency in the presence of a mutant hemagglutinin complex protein of the present disclosure.Type: ApplicationFiled: June 29, 2015Publication date: May 11, 2017Applicant: Osaka UniversityInventors: Masahiro Kinooka, Meehae Kim, Yukako Fujinaga, Yo Sugawara
-
Publication number: 20150329831Abstract: Provided is a method by which cells deviated from the undifferentiated state, which emerge in a colony during culture of stem cells having pluripotency, can be removed. In one aspect, provided is a method for culturing stem cells having pluripotency, the method including performing cell culture in the presence of a substance that can inhibit cell-cell adhesion. In another aspect, provided is a method for removing cells deviated from the undifferentiated state, the cells being cells that have emerged or may possibly emerge during culture of stem cells having pluripotency, the method including performing cell culture in the presence of a substance that can inhibit cell-cell adhesion. In still another aspect, provided is a method for maintaining the undifferentiated state of stem cells having pluripotency, the method including performing cell culture in the presence of a substance that can inhibit cell-cell adhesion.Type: ApplicationFiled: December 26, 2013Publication date: November 19, 2015Applicant: OSAKA UNIVERSITYInventors: Masahiro Kinooka, Meehae Kim, Yukako Fujinaga, Yo Sugawara