Abstract: The present disclosure provides a system for automatically constructing and providing microbial genome data. This method for processing sequence information about a single biological unit includes: (A) a step for performing clustering, for each same lineage, on partial sequence information (in a slide, SAG) about a genome (or an equivalent gene set) of a plurality of single biological units (for example, cells), on the basis of a sequence (16S rRNA or a marker gene) for identifying biological lineage; and (B) a step for performing collation with information about a genome of the single biological units in a database, if necessary.

Abstract: The present disclosure provides a technique enabling analysis of cell viability information and nucleic acid information together for each single cell in a cell population when analyzing the composition of the cell population. The present disclosure discloses a method that is for analyzing the composition of a cell population and that comprises: a step for providing gel capsules in each of which a single cell of the cell population is encapsulated in a state of being labeled with a labeling agent, where the labeling agent distinctively labels a living cell and a dead cell; a step for amplifying a nucleic acid derived from each single cell of the cell population; a step for obtaining cell viability information for each single cell of the cell population; and a step for analyzing the amplified nucleic acid derived from each single cell of the cell population.

Abstract: The present invention provides a genome library production method in which cell lysis and genome amplification are performed using a simple operation. More particularly, the present invention provides a method that is for amplifying polynucleotides in cells and that comprises: a step for using a sample containing two or more cells or cell-like structures, and encapsulating the cells or cell-like structures into droplets, one for each droplet; a step for gelling the droplets to generate gel capsules; a step for performing lysis of the cells or cell-like structures by immersing the gel capsules in one or more types of reagents for lysis so as to cause the polynucleotides in the cells to be eluted in the gel capsules and to be kept in the gel capsules in a state where substances binding to the polynucleotides are removed; and a step for bringing the polynucleotides into contact with a reagent for amplification to amplify the polynucleotides in the gel capsules.

Abstract: The present invention provides a genome library production method in which cell lysis and genome amplification are performed using a simple operation. More particularly, the present invention provides a method that is for amplifying polynucleotides in cells and that comprises: a step for using a sample containing two or more cells or cell-like structures, and encapsulating the cells or cell-like structures into droplets, one for each droplet; a step for gelling the droplets to generate gel capsules; a step for performing lysis of the cells or cell-like structures by immersing the gel capsules in one or more types of reagents for lysis so as to cause the polynucleotides in the cells to be eluted in the gel capsules and to be kept in the gel capsules in a state where substances binding to the polynucleotides are removed; and a step for bringing the polynucleotides into contact with a reagent for amplification to amplify the polynucleotides in the gel capsules.

Abstract: The purpose of the present invention is to provide a sample collection system capable of repeatedly collecting a sample. The present invention discloses a system including: at least one or multiple hollow collection needle(s) each having a knife edge for collecting the sample from a biological specimen; and a syringe, a solenoid and a reservoir that serve as a liquid-inflow mechanism allowing a solution to flow into the collection needle, wherein the sample is ejected from the collection needle together with the solution so as to recover the sample.

Abstract: A cell trapping device includes a housing that includes an inlet opening connected to an inlet line through which a cell dispersion liquid is introduced and an outlet opening connected to an outlet line through which the cell dispersion liquid is discharged; and a filter which is positioned within the housing and includes a trapping region for trapping cancer cells contained in the cell dispersion liquid. The filter is bonded to the housing, at least a part of the trapping region is formed of an observation region for observing the trapping region from the outside, the inlet line and the inlet opening are arranged at outer positions than the observation region when viewed from a normal line direction of the filter, and the inlet line is extended along an in-plane direction of the filter.

Abstract: The instant invention is directed to a microfluidic device which separates and captures with high efficiency a large amount of cells in a sample at one-cell level without damaging the cells by utilizing a microfabrication technology.

Abstract: It is to provide a microfluidic device which separates and captures with high efficiency a large amount of cells in a sample at one-cell level without damaging the cells by utilizing a microfabrication technology.