Abstract: The present invention provides a DNA coding for a protein defined in the following (A) or (B) is obtained from Brevibacterium flavum chromosomal DNA library by cloning a DNA fragment that complicates serB deficiency of Escherichia coli as a open reading frame in the DNA fragment. (A) A protein which comprises an amino acid sequence of SEQ ID: 2 in Sequence Listing; or (B) A protein which comprises an amino acid sequence including substitution, deletion, insertion, addition or inversion of one or several amino acids in the amino acid sequence of SEQ ID NO: 2 in Sequence Listing, and which has phosphoserine phosphatase activity.

Abstract: A structure is provided between a first columnar structural member and at least a second structural member in which the stress concentration at the fixed end portion of the reinforcing member with the first columnar structural member and the second structural member(s) is greatly relaxed. Also, in the case where the reinforcing member is welded, the residual welding stress is relaxed, with the result that the proof stress and the fatigue performance are remarkably improved over the prior art. In the joining structure with the first columnar structural member and the second structural member(s), the first columnar structural member and the second structural member(s) joined to each other have welded thereto at least a tabular reinforcing member to reinforce the joining of the structural members. The reinforcing member has a U- or V-shaped bent portion along the surface of the structural members and also an opening.

Abstract: The present invention provides a DNA coding for a protein defined in the following (A) or (B) is obtained from Brevibacterium flavum chromosomal DNA library by cloning a DNA fragment that complicates serB deficiency of Escherichia coli as a open reading frame in the DNA fragment. (A) A protein which comprises an amino acid sequence of SEQ ID: 2 in Sequence Listing; or (B) A protein which comprises an amino acid sequence including substitution, deletion, insertion, addition or inversion of one or several amino acids in the amino acid sequence of SEQ ID NO: 2 in Sequence Listing, and which has phosphoserine phosphatase activity.

Abstract: The present invention provides a DNA coding for a protein defined in the following (A) or (B) is obtained from Brevibacterium flavum chromosomal DNA library by cloning a DNA fragment that complicates serB deficiency of Escherichia coli as a open reading frame in the DNA fragment. (A) A protein which comprises an amino acid sequence of SEQ ID: 2 in Sequence Listing; or (B) A protein which comprises an amino acid sequence including substitution, deletion, insertion, addition or inversion of one or several amino acids in the amino acid sequence of SEQ ID NO: 2 in Sequence Listing, and which has phosphoserine phosphatase activity.

Abstract: The present invention: provides a steel pipe pole base and a method for reinforcing the steel pipe pole base that do not allow the strength to lower in the vicinity of the weld toe of a rib even in the event of applying repeated bending moment; and is characterized by forming peening processed portions 20 by ultrasonic vibration at the weld toes 16 of tabular ribs 12 welded to the base of a steel pipe pole 10 in the form of a T-joint or the weld toes of inverted-U shaped ribs 13 or inverted-V shaped ribs 14, the ribs being bent at the upper end portions, welded to the base of a steel pipe pole 10 in the form of a T-joint. Preferable treatment conditions are 20 to 50 ?m in amplitude and 10 to 50 kHz in frequency. In particular, the present invention makes it possible to remarkably improve the fatigue property because stress concentration is relaxed by the synergetic effect of bending the upper end portions 16 of the ribs 13 and applying a peening treatment.

Abstract: A gene encoding a protein constituting sucrose PTS of coryneform bacterium is provided by amplifying a region existing downstream from sucrase gene in coryneform bacterium by the cassette-ligation mediated PCR to obtain DNA encoding sucrose PTS enzyme II, which is a protein defined in the following (A) or (B): (A) a protein which has the amino acid sequence of SEQ ID NO: 2 in Sequence Listing; (B) a protein which has the amino acid sequence of SEQ ID NO: 2 in Sequence Listing including substitution, deletion, insertion, addition or inversion of one or several amino acids, and an activity for binding to sucrose.

Abstract: The present invention provides a joining structure capable of greatly improving the proof stress and the fatigue property by alleviating the stress concentration and residual stress caused by welding heat at one or both ends of a tabular member. In the present invention, one or both ends 4 of a tabular member 3 such as a reinforcing rib, fixed to the surface of a structural member 1 in the direction of the principal stress of the structural member 1 so as to protrude in the shape of T, is/are bent in a direction deviating from the direction of the principal stress and, by this, the rigidity at the end(s) 4 of the tabular member 3 decreases and the stress concentration is alleviated. It is preferable to bend one or both ends of a tabular member 3 in the shape of an gradual curve and to the extent that each bent end is formed at a right angle to the direction of the principal stress. The tabular member may have the shape of a flat plate, or it may be bent so that it has the shape of U or V as a whole.

Abstract: A structure is provided between a first columnar structural member and at least a second structural member is disclosed in which the stress concentration at the fixed end portion of the reinforcing member with the first columnar structural member and the second structural member(s) is greatly relaxed. Also, in the case where the reinforcing member is welded, the residual welding stress is remarkably relaxed, with the result that the proof stress and the fatigue performance are remarkably improved over the prior art. In the joining structure with the first columnar structural member and the second structural member(s), the first columnar structural member and the second structural member(s) joined to each other have welded thereto at least a tabular reinforcing member to reinforce the joining of the structural members. The reinforcing member has a U- or V-shaped bent portion along the surface of the structural members and also an opening.

Abstract: A coryneform bacterium which has enhanced intracellular pyruvate carboxylase activity obtained by increasing copy number of a gene encoding the intracellular pyruvate carboxylase, or by enhancing function of a expression regulatory sequence for the gene, and has L-glutamic acid-producing ability is cultured in a medium so that L-glutamic acid should be produced and accumulated in the culture, and L-glutamic acid is collected from the culture.

Abstract: A gene coding for fructose phosphotransferase is introduced into a coryneform bacterium having an ability to produce an L-amino acid such as L-lysine or L-glutamic acid to enhance fructose phosphotransferase activity and thereby improve the L-amino acid producing ability.

Abstract: The object of the present invention is to produce a polymer, which is free from the troubles of the coloration and the decrease in the mechanical properties of molded articles (films, etc.), crosslinkage failure, migration (stain) or the like, by the living radical polymerization method wherein the content of impurities consisting of a transition metal and its ligand employed as a polymerization activator is reduced. A process for producing a polymer is described, comprising: a) the step of performing a polymerization reaction by using a monomer, a transition metal and its ligand and a polymerization initiator to form a polymer; and b) the step of eliminating the transition metal and its ligand contained in the polymer under the condition of a viscosity of 100 Pa·s or below, wherein the step b comprises: b1) the former step of eliminating the transition metal and its ligand insoluble in the polymer; and b2) the latter step of eliminating the transition metal and its ligand dissolved in the polymer.

Abstract: L-serine is produced by cultivating in a medium a coryneform bacterium having L-serine productivity in which an activity of at least one of phosphoserine phosphatase and phosphoserine transaminase is enhanced, preferably, further having introduced therein a gene coding for D-3-phosophoglycerate dehydrogenase in which feedback inhibition by L-serine is desensitizied, allowing L-serine to accumulate in the medium, and collecting the L-serine from the medium.

Abstract: A coryneform bacterium which has enhanced intracellular pyruvate carboxylase activity obtained by increasing copy number of a gene encoding the intracellular pyruvate carboxylase, or by enhancing function of a expression regulatory sequence for the gene, and has L-glutamic acid-producing ability is cultured in a medium so that L-glutamic acid should be produced and accumulated in the culture, and L-glutamic acid is collected from the culture.

Abstract: The present invention provides a DNA coding for a protein defined in the following (A) or (B) is obtained from Brevibacterium flavum chromosomal DNA library by cloning a DNA fragment that complicates serB deficiency of Escherichia coli as a open reading frame in the DNA fragment.

Abstract: The L-lysine-producing ability and the L-lysine-producing speed are improved in a coryneform bacterium harboring an aspartokinase in which feedback inhibition by L-lysine and L-threonine is substantially desensitized, by successively enhancing DNA coding for a dihydrodipicolinate reductase, DNA coding for a dihydrodipicolinate synthase, DNA coding for a diaminopimelate decarboxylase, and DNA coding for a diaminopimelate dehydrogenase.

Abstract: Disclosed is a coryneform bacterium having resistance to azaserine or &bgr;-(2-thienyl)-DL-alanine and having L-serine productivity.

Abstract: A circuit board for mounting a bare chip in the form of a flip chip. A metallic foil for protecting circuits in a state insulated therefrom is arranged in an area where the bare chip is located.

Abstract: The present invention provides a DNA coding for a protein defined in the following (A) or (B) is obtained from Brevibacterium flavum chromosomal DNA library by cloning a DNA fragment that complicates serB deficiency of Escherichia coli as a open reading frame in the DNA fragment.

Abstract: A low thermal expansion circuit board 1 on which a semiconductor element can be mounted with ease and high reliability, which comprises an insulating layer 3 having an Ni—Fe—based alloy foil or a titanium foil as a core, a wiring conductor 4 on both sides thereof, and an adhesive resin layer 5 on the side on which a semiconductor element is to be mounted.

Abstract: The L-lysine-producing ability and the L-lysine-producing speed are improved in a coryneform bacterium harboring an aspartokinase in which feedback inhibition by L-lysine and L-threonine is substantially desensitized, by successively enhancing DNA coding for a dihydrodipicolinate reductase, DNA coding for a dihydrodipicolinate synthase, DNA coding for a diaminopimelate decarboxylase, and DNA coding for a diaminopimelate dehydrogenase.