Patents by Inventor Masaki Torimura
Masaki Torimura has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Patent number: 12117343Abstract: Provided is a sample analysis system including: a droplet device configured to intermittently introduce a sample to a measurement region set in plasma; a light emission detection device configured to detect light emission in the measurement region at a detection timing, the detection timing being set at a predetermined cycle in advance; and an analysis device configured to analyze the sample based on the detected light emission, wherein the analysis device is provided with: a distribution computing unit configured to compute a time-spatial light intensity distribution based on the detected light emission, the time-spatial light intensity distribution being a distribution of a light intensity according to the detection timing, a position in the measurement region, and a wavelength component of the light emission; and a characteristic specifying unit configured to compute, from the time-spatial light intensity distribution, a feature amount that correlates with a sample characteristic indicating a property of tType: GrantFiled: December 10, 2020Date of Patent: October 15, 2024Assignee: NATIONAL INSTITUTE OF ADVANCED INDUSTRIAL SCIENCE AND TECHNOLOGYInventors: Yoshiyuki Teramoto, Masaki Torimura, Taizo Sano
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Publication number: 20230070781Abstract: A sample analysis system including: a droplet device that intermittently introduces a sample to a measurement region set in plasma; a light emission detection device that detects light emission in the measurement region at a detection timing, the detection timing being set at a predetermined cycle in advance; and an analysis device that analyzes the sample based on the detected light emission, the analysis device being provided with: a distribution computing unit that computes a time-spatial light intensity distribution based on the detected light emission, the time-spatial light intensity distribution being a distribution of a light intensity according to the detection timing, a position in the measurement region, and an wavelength component of the light emission; and a characteristic specifying unit that computes a feature amount that correlates with a sample characteristic indicating a property of the sample and specifies the sample characteristic based on the feature amount.Type: ApplicationFiled: December 10, 2020Publication date: March 9, 2023Applicant: National Institute of Advanced Industrial Science and TechnologyInventors: Yoshiyuki TERAMOTO, Masaki TORIMURA, Taizo SANO
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Patent number: 10663456Abstract: The present invention provides means for evaluating harmfulness of a chemical substance before occurrence of cell death, that is, more quickly and sensitively compared to conventional methods wherein the remaining viable cell count is determined using a reductive coloring reagent after a period of time required for occurrence of cell death due to the chemical substance. A double-stranded RNA probe comprising an RNA strand labeled with a fluorescent dye A that emits fluorescence, and an RNA strand labeled with a fluorescent dye B that quenches emission from a fluorescent dye in the vicinity thereof, wherein the fluorescence is quenched in a double-stranded state due to occurrence of fluorescence resonance energy transfer (FRET) between the two kinds of fluorescent dyes.Type: GrantFiled: July 6, 2016Date of Patent: May 26, 2020Assignee: NATIONAL INSTITUTE OF ADVANCED INDUSTRIAL SCIENCE AND TECHNOLOGYInventors: Hidenori Tani, Masaki Torimura, Hiroaki Sato
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Patent number: 10533231Abstract: The invention relates to establishment of a series of artificial luciferases based on artificial amino acid sequences extracted by amino acid alignment of copepod-derived luciferase sequences in a database based on amino acid similarity. The invention provides high luminescence intensity, high luminescence stability, and a spectrum with increased wavelength as luminescence characteristics. A series of artificial luciferases (ALuc) was established. The group of ALucs has superior luminescence characteristics, such as an increase in luminescence intensity, an increase in luminescence stability, or an increase in wavelength of the luminescence spectrum, which were not obtained before. Further, by using the artificial luciferases (ALuc) of the invention, it is possible to provide a novel, superior bioassay system, such as a bioluminescent probe, two-hybrid assay, a luminescent capsule, or the like having improved measurement function.Type: GrantFiled: September 18, 2013Date of Patent: January 14, 2020Assignee: National Institute of Advanced Industrial Science and TechnologyInventors: Sung Bae Kim, Masaki Torimura, Hiroaki Tao, Tetsuya Nakazato
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Patent number: 10214766Abstract: The invention relates to a bioluminescent substrate suitably usable in a series of artificial luciferases (ALuc), and the invention provides a wavelength-shifted spectrum with a selective high intensity luminescence and high luminescence stability obtained by the use of the substrate together with ALuc. The luminescent substrate for ALuc obtained by the invention can be included together with a suitable luminescence solution in a luminescence kit. The bioluminescent substrate for ALuc of the invention can exhibit unprecedented excellent luminescence specificity and functionality in the conventional bioluminescence probe, two-hybrid assay, bioluminescent capsule, and reporter gene assay.Type: GrantFiled: October 16, 2014Date of Patent: February 26, 2019Assignee: NATIONAL INSTITUTE OF ADVANCED INDUSTRIAL SCIENCE AND TECHNOLOGYInventors: Sung Bae Kim, Hiroshi Izumi, Hiroaki Tao, Masaki Torimura, Akihiro Wakisaka
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Publication number: 20180217128Abstract: The present invention provides means for evaluating harmfulness of a chemical substance before occurrence of cell death, that is, more quickly and sensitively compared to conventional methods wherein the remaining viable cell count is determined using a reductive coloring reagent after a period of time required for occurrence of cell death due to the chemical substance. A double-stranded RNA probe comprising an RNA strand labeled with a fluorescent dye A that emits fluorescence, and an RNA strand labeled with a fluorescent dye B that quenches emission from a fluorescent dye in the vicinity thereof, wherein the fluorescence is quenched in a double-stranded state due to occurrence of fluorescence resonance energy transfer (FRET) between the two kinds of fluorescent dyes.Type: ApplicationFiled: July 6, 2016Publication date: August 2, 2018Applicant: NATIONAL INSTITUTE OF ADVANCED INDUSTRIAL SCIENCE AND TECHNOLOGYInventors: Hidenori TANI, Masaki TORIMURA, Hiroaki SATO
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Publication number: 20160281129Abstract: The invention relates to a bioluminescent substrate suitably usable in a series of artificial luciferases (ALuc), and the invention provides a wavelength-shifted spectrum with a selective high intensity luminescence and high luminescence stability obtained by the use of the substrate together with ALuc. The luminescent substrate for ALuc obtained by the invention can be included together with a suitable luminescence solution in a luminescence kit. The bioluminescent substrate for ALuc of the invention can exhibit unprecedented excellent luminescence specificity and functionality in the conventional bioluminescence probe, two-hybrid assay, bioluminescent capsule, and reporter gene assay.Type: ApplicationFiled: October 16, 2014Publication date: September 29, 2016Applicant: NATIONAL INSTITUTE OF ADVANCED INDUSTRIAL SCIENCE AND TECHNOLOGYInventors: Sung Bae KIM, Hiroshi IZUMI, Hiroaki TAO, Masaki TORIMURA, Akihiro WAKISAKA
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Publication number: 20150284813Abstract: The invention relates to establishment of a series of artificial luciferases based on artificial amino acid sequences extracted by amino acid alignment of copepod-derived luciferase sequences in a database based on amino acid similarity. The invention provides high luminescence intensity, high luminescence stability, and a spectrum with increased wavelength as luminescence characteristics. A series of artificial luciferases (ALuc) was established. The group of ALucs has superior luminescence characteristics, such as an increase in luminescence intensity, an increase in luminescence stability, or an increase in wavelength of the luminescence spectrum, which were not obtained before. Further, by using the artificial luciferases (ALuc) of the invention, it is possible to provide a novel, superior bioassay system, such as a bioluminescent probe, two-hybrid assay, a luminescent capsule, or the like having improved measurement function.Type: ApplicationFiled: September 18, 2013Publication date: October 8, 2015Inventors: Sung Bae Kim, Masaki Torimura, Hiroaki Tao, Tetsuya Nakazato
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Patent number: 9120071Abstract: A spotter that includes a plurality of spotting heads, each of the plurality of spotting heads having a discharging portion at a tip portion, the plurality of spotting heads form an m×n array (m, n>1) with m spotting heads arranged lengthwise and n spotting heads arranged crosswise; and a pitch varying mechanism configured to vary an array pitch of the plurality of spotting heads arrayed in a lengthwise direction and an array pitch of the plurality of spotting heads arrayed in a crosswise direction.Type: GrantFiled: May 31, 2013Date of Patent: September 1, 2015Assignee: NATIONAL INSTITUTE OF ADVANCED INDUSTRIAL SCIENCE AND TECHNOLOGYInventors: Hiroshi Aoki, Masaki Torimura, Hiroaki Tao, Takashi Ikeda
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Patent number: 8808625Abstract: In a dispensing apparatus for dispensing a liquid, such as a sample solution, on a substrate, such as a glass slide, the dispensing apparatus includes a capillary provided with a distal end and a proximal end, a pump unit configured to pump an operating liquid into the capillary and to pump the operating liquid out of the capillary, and a controller configured to control the pump unit so as to change a position of a liquid surface of the operating liquid in the capillary so that a predetermined volume of liquid is suctioned from the distal end into the capillary and the liquid suctioned in the capillary is discharged from the distal end. As a result, it is possible to precisely dispense extremely small volume amounts of liquids such as a nanoliter.Type: GrantFiled: August 29, 2008Date of Patent: August 19, 2014Assignee: National Institute of Advanced Industrial Science and TechnologyInventors: Hiroshi Aoki, Hiroaki Tao, Masaki Torimura, Takashi Ikeda
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Patent number: 8663577Abstract: A spotter that includes a plurality of spotting heads, each of the plurality of spotting heads having a discharging portion at a tip portion, the plurality of spotting heads form an m×n array (m, n>1) with m spotting heads arranged lengthwise and n spotting heads arranged crosswise; and a pitch varying mechanism configured to vary an array pitch of the plurality of spotting heads arrayed in a lengthwise direction and an array pitch of the plurality of spotting heads arrayed in a crosswise direction.Type: GrantFiled: April 17, 2009Date of Patent: March 4, 2014Assignee: National Institute of Advanced Industrial Science and TechnologyInventors: Hiroshi Aoki, Masaki Torimura, Hiroaki Tao, Takashi Ikeda
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Publication number: 20130261029Abstract: A spotter that includes a plurality of spotting heads, each of the plurality of spotting heads having a discharging portion at a tip portion, the plurality of spotting heads form an m×n array (m, n>1) with m spotting heads arranged lengthwise and n spotting heads arranged crosswise; and a pitch varying mechanism configured to vary an array pitch of the plurality of spotting heads arrayed in a lengthwise direction and an array pitch of the plurality of spotting heads arrayed in a crosswise direction.Type: ApplicationFiled: May 31, 2013Publication date: October 3, 2013Applicant: National Institute of Advanced Industrial Science and TechnologyInventors: Hiroshi AOKI, Masaki TORIMURA, Hiroaki TAO, Takashi IKEDA
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Publication number: 20090305910Abstract: At each lattice point 2 of pantographs 1, a capillary 3 is held perpendicular to the pantograph lattice. The pantograph 1 includes: rings 4 and 5 to serve as lattice points; and shafts 6 linking the rings with one another. Each of the capillaries 3 is supported by the two rings 4 and 5. The shafts are free to rotate about an intersection 7. The ring 5 is fixed to the capillary 3, but the ring 4 is a movable ring being moveable freely up and down in the axial direction of the capillary 3 in conjunction with the pantograph 1. The lattice distance can be determined accomplished by moving up or down the movable ring at an arbitrarily-chosen lattice point in a pantograph 1. Accordingly, all the capillaries move in parallel to one another, and the ends of all the capillaries always stay within a single plane.Type: ApplicationFiled: June 27, 2007Publication date: December 10, 2009Applicant: NATIONAL INSTITUTE OF ADVANCED INDUSTRIAL SCIENCE AND TECHNOLOGYInventors: Hiroshi Aoki, Masaki Torimura, Hiroaki Tao, Takashi Ikeda
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Publication number: 20090233816Abstract: At each lattice point of pantographs, a capillary is held perpendicular to the pantograph lattice. The pantograph includes: rings to serve as lattice points; and shafts linking the rings with one another. Each of the capillaries is supported by the two rings. The shafts are free to rotate about an intersection. The ring is fixed to the capillary, but the ring is a movable ring being moveable freely up and down in the axial direction of the capillary in conjunction with the pantograph. The lattice distance can be determined accomplished by moving up or down the movable ring at an arbitrarily-chosen lattice point in a pantograph. Accordingly, all the capillaries move in parallel to one another, and the ends of all the capillaries always stay within a single plane.Type: ApplicationFiled: April 17, 2009Publication date: September 17, 2009Applicant: National Institute of Advanced Industrial Science and TechnologyInventors: Hiroshi Aoki, Masaki Torimura, Hiroaki Tao, Takashi Ikeda
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Publication number: 20090180930Abstract: In a dispensing apparatus for dispensing a liquid, such as a sample solution, on a substrate, such as a glass slide, the dispensing apparatus includes a capillary provided with a distal end and a proximal end, a pump unit configured to pump an operating liquid into the capillary and to pump the operating liquid out of the capillary, and a controller configured to control the pump unit so as to change a position of a liquid surface of the operating liquid in the capillary so that a predetermined volume of liquid is suctioned from the distal end into the capillary and the liquid suctioned in the capillary is discharged from the distal end. As a result, it is possible to precisely dispense extremely small volume amounts of liquids such as a nanoliter.Type: ApplicationFiled: August 29, 2008Publication date: July 16, 2009Applicant: National Institute of Advanced Industrial Science and TechnologyInventors: Hiroshi AOKI, Hiroaki TAO, Masaki TORIMURA, Takashi IKEDA
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Patent number: 7521672Abstract: Provided are a laser desorption ionization mass spectrometry sample plate for a soft LDI-MS measurement, in which when a laser beam is irradiated, a correct measurement of high sensitivity can be made without generation of any disturbance peak and uniform coating of a sample can be made on a sample plate in fabrication of the sample, and a measurement apparatus using the sample plate. A specified ionization element having a dot structure is used as an ionization medium which is used in laser desorption ionization mass spectrometry and absorbs a laser beam.Type: GrantFiled: April 27, 2006Date of Patent: April 21, 2009Assignee: National Institute of Advanced Industrial Science and TechnologyInventors: Hiroaki Sato, Atsushi Yamamoto, Teruyuki Seino, Masaki Torimura, Hiroaki Tao
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Patent number: 7354707Abstract: Nucleic acid probes are provided, each of which is formed of a single-stranded oligonucleotide which can hybridize to a target nucleic acid and is labeled with a fluorescent dye or with a fluorescent dye and a quencher substance. The nucleic acid probes can be easily designed, permit determination, polymorphous analysis or real-time quantitative PCR of nucleic acids in short time, and are not dissociated during reactions. Nucleic acid determination methods, polymorphous analysis methods and real-time quantitative PCR methods, which make use of the nucleic acid probes, are also provided.Type: GrantFiled: June 27, 2001Date of Patent: April 8, 2008Assignees: National Institute of Advanced Industrial Science and Technology, Kankyo Engineering Co., Ltd.Inventors: Ryuichiro Kurane, Takahiro Kanagawa, Yoichi Kamagata, Masaki Torimura, Shinya Kurata, Kazutaka Yamada, Toyokazu Yokomaku
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Patent number: 7273700Abstract: A novel nucleic acid probe for nucleic acid determination includes a single-stranded nucleic acid labeled with plural fluorescent dyes containing at least one pair of fluorescent dyes to induce FRET, the pair of fluorescent dyes including a fluorescent dye (a donor dye) capable of serving as a donor dye and a fluorescent dye (an acceptor dye) capable of serving as an acceptor dye, in which the nucleic acid probe has such a base sequence and is labeled with the fluorescent dyes so that the fluorescence intensity of the acceptor dye decreases upon hybridization with a target nucleic acid. A novel nucleic acid determination method uses the probe. The probe and method can determine one or more types of target nucleic acids in an assay system in parallel using a simple apparatus.Type: GrantFiled: March 27, 2002Date of Patent: September 25, 2007Assignees: National Institute of Advanced Industrial Science and Technology, Kankyo Engineering Co., Ltd.Inventors: Ryuichiro Kurane, Takahiro Kanagawa, Yoichi Kamagata, Masaki Torimura, Shinya Kurata, Kazutaka Yamada, Toyokazu Yokomaku
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Publication number: 20060255262Abstract: Provided are a laser desorption ionization mass spectrometry sample plate for a soft LDI-MS measurement, in which when a laser beam is irradiated, a correct measurement of high sensitivity can be made without generation of any disturbance peak and uniform coating of a sample can be made on a sample plate in fabrication of the sample, and a measurement apparatus using the sample plate. A specified ionization element having a dot structure is used as an ionization medium which is used in laser desorption ionization mass spectrometry and absorbs a laser beam.Type: ApplicationFiled: April 27, 2006Publication date: November 16, 2006Inventors: Hiroaki Sato, Atsushi Yamamoto, Teruyuki Seino, Masaki Torimura, Hiroaki Tao
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Publication number: 20050048485Abstract: A novel nucleic acid probe for nucleic acid determination includes a single-stranded nucleic acid labeled with plural fluorescent dyes containing at least one pair of fluorescent dyes to induce FRET, the pair of fluorescent dyes including a fluorescent dye (a donor dye) capable of serving as a donor dye and a fluorescent dye (an acceptor dye) capable of serving as an acceptor dye, in which the nucleic acid probe has such a base sequence and is labeled with the fluorescent dyes so that the fluorescence intensity of the acceptor dye decreases upon hybridization with a target nucleic acid. A novel nucleic acid determination method uses the probe. The probe and method can determine one or more types of target nucleic acids in an assay system in parallel using a simple apparatus.Type: ApplicationFiled: March 27, 2002Publication date: March 3, 2005Inventors: Ryuichiro Kurane, Takahiro Kanagawa, Yoichi Kamagata, Masaki Torimura, Shinya Kurata, Kazutaka Yamada, Toyokazu Yokomaku