Abstract: Provided is a method for detecting a stem cell based on an undifferentiated sugar chain marker having a specific sugar chain structure, wherein the stem cell is detected by detecting podocalyxin contained in a culture supernatant or a lysate of cells by a “lectin-antibody sandwich method” using a combination of a lectin and an antibody and having high sensitivity, the method including steps of: contacting the culture supernatant or the lysate, a lectin capable of binding to a sugar chain represented by (Formula 1) or (Formula 2), and an antibody capable of binding to keratan sulfate to form a complex composed of the lectin, podocalyxin and the antibody; and detecting the complex. wherein R1 represents an OH group or any sugar chain and R2 represents an OH group or any sugar chain, protein, lipid, or another molecule. wherein R1 represents an OH group or any sugar chain and R2 represents an OH group or any sugar chain, protein, lipid, or another molecule.

Abstract: Provided is a method for detecting a stem cell based on an undifferentiated sugar chain marker having a specific sugar chain structure, wherein the stem cell is detected by detecting podocalyxin contained in a culture supernatant or a lysate of cells by a “lectin-antibody sandwich method” using a combination of a lectin and an antibody and having high sensitivity, the method including steps of: contacting the culture supernatant or the lysate, a lectin capable of binding to a sugar chain represented by (Formula 1) or (Formula 2), and an antibody capable of binding to keratan sulfate to form a complex composed of the lectin, podocalyxin and the antibody; and detecting the complex. wherein R1 represents an OH group or any sugar chain and R2 represents an OH group or any sugar chain, protein, lipid, or another molecule. wherein R1 represents an OH group or any sugar chain and R2 represents an OH group or any sugar chain, protein, lipid, or another molecule.

Abstract: An object of the present invention is to provide a method for evaluating a differentiation status of cells using a culture supernatant of stem cells. Provided is “undifferentiation sugar chain marker” composed of the sugar chain structure “Fuc?1-2Gal?1-3GlcNAc” or “Fuc?1-2Gal?1-3GalNAc” and capable of sensitively determining the undifferentiated state of stem cells using a culture supernatant of stem cells. Also found is BC2LCN lectin or a modified product thereof capable of sensitively recognizing the “undifferentiation sugar chain marker” as excellent “probe for detecting the undifferentiation sugar chain marker” capable of determining the undifferentiation status of cells using a culture supernatant.

Abstract: Provided are a method for accurately evaluating the differentiation status of stem cells by selectively staining only stem cells in an undifferentiated state, and a method for positively isolating only stem cells in an undifferentiated state. Specifically provided is a method for determining differentiation of a cell comprising a step of contacting a test cell with a probe comprising protein (A) or (B) below and a step of detecting the presence of binding of the probe to the test cell. The method for determining differentiation of a cell is capable of detecting the presence or absence of an undifferentiated stem cell in test cells by using a probe that specifically reacts with undifferentiated stem cells and detecting the presence of bonding to the test cell.

Abstract: An object of the present invention is to provide a method for evaluating a differentiation status of cells using a culture supernatant of stem cells. Provided is “undifferentiation sugar chain marker” composed of the sugar chain structure “Fuc?1-2Gal?1-3GlcNAc” or “Fuc?1-2Gal?1-3GalNAc” and capable of sensitively determining the undifferentiated state of stem cells using a culture supernatant of stem cells. Also found is BC2LCN lectin or a modified product thereof capable of sensitively recognizing the “undifferentiation sugar chain marker” as excellent “probe for detecting the undifferentiation sugar chain marker” capable of determining the undifferentiation status of cells using a culture supernatant.

Abstract: Provided are a method for accurately evaluating the differentiation status of stem cells by selectively staining only stem cells in an undifferentiated state, and a method for positively isolating only stem cells in an undifferentiated state. Specifically provided is a method for determining differentiation of a cell comprising a step of contacting a test cell with a probe comprising protein (A) or (B) below and a step of detecting the presence of binding of the probe to the test cell. The method for determining differentiation of a cell is capable of detecting the presence or absence of an undifferentiated stem cell in test cells by using a probe that specifically reacts with undifferentiated stem cells and detecting the presence of bonding to the test cell.

Abstract: The present invention provides compositions and methods for inducing neuronal cell differentiation.

Abstract: The present invention provides compositions and methods for inducing neuronal cell differentiation.

Abstract: The present invention provides compositions and methods for inducing neuronal cell differentiation.

Abstract: The present invention provides compositions and methods for inducing neuronal cell differentiation.

Abstract: A stable linkage between a genotype and a phenotype in a cell-free system was successfully achieved by using interaction between a RNA-binding protein and RNA, between a DNA-binding protein and DNA, or by using a protein that inactivates a ribosome. Furthermore, it was found that functional proteins could be selected by using these stable linkages.

Abstract: An object of the present invention is to provide a maxizyme that can bind to a target mRNA regardless of its conformation, and can effectively cleave the mRNA. The present invention provides a maxizyme which binds to a molecule having helicase activity.

Abstract: This invention relates to a chimeric molecule comprising a region with binding affinity for a molecule capable of sliding and any functional region; a chimeric molecule comprising a molecule capable of sliding and any functional region; a chimeric molecule comprising a region with binding affinity for a molecule forming a complex with a molecule capable of sliding, and any functional region; a chimeric molecule comprising a protein capable of sliding and any functional nucleic acid; a chimeric molecule comprising a nucleic acid with binding affinity for a protein capable of sliding or a nucleic acid with binding affinity for a molecule forming a complex with said protein, and any functional nucleic acid; a vector comprising DNA encoding the chimeric molecule; and use thereof in medicaments, etc.

Abstract: The present invention relates to a nucleic acid enzyme which has a sensor site and a cleavage active site, wherein the nucleic acid enzyme exhibits a cleavage activity towards a target RNA only when the target RNA binds to the cleavage active site while another RNA, DNA or protein binds to the sensor site, and use of the enzyme. According to the present invention, the nucleic acid enzyme has an RNA-cleaving activity by which harmful cells such as infected or cancerated cells can be killed selectively and efficiently. Furthermore, the nucleic acid enzyme has an RNA-cleaving ability that can be regulated by a cell-specifically or time-specifically expressed RNA, DNA or protein or by an artificially introduced RNA, DNA or protein.