Patents by Inventor Masako Shinjoh

Masako Shinjoh has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Patent number: 9279138
    Abstract: The present invention relates to newly identified genes that encode proteins that are involved in the synthesis of L-ascorbic acid (hereinafter also referred to as Vitamin C). The invention also features polynucleotides comprising the full-length polynucleotide sequences of the novel genes and fragments thereof, the novel polypeptides encoded by the polynucleotides and fragments thereof, as well as their functional equivalents. The present invention also relates to the use of the polynucleotides and polypeptides as biotechnological tools in the production of Vitamin C from microorganisms, whereby a modification of the polynucleotides and/or encoded polypeptides has a direct or indirect impact on yield, production, and/or efficiency of production of the fermentation product in the microorganism. Also included are methods/processes of using the polynucleotides and modified polynucleotide sequences to transform host microorganisms.
    Type: Grant
    Filed: February 10, 2006
    Date of Patent: March 8, 2016
    Assignee: DSM ASSETS B.V.
    Inventors: Tatsuo Hoshino, Masako Shinjoh, Christine Toepfer, Noribumi Tomiyama
  • Patent number: 9115378
    Abstract: The present invention relates to newly identified microorganisms capable of direct production of L-ascorbic acid (hereinafter also referred to as Vitamin C). The invention also relates to polynucleotide sequences comprising genes that encode proteins which are involved in the synthesis of Vitamin C. The invention also features polynucleotides comprising the full length polynucleotide sequences of the novel genes and fragments thereof, the novel polypeptides encoded by the polynucleotides and fragments thereof, as well as their functional equivalents. The present invention also relates to the use of said polynucleotides and polypeptides as biotechnological tools in the production of Vitamin C from microorganisms, whereby a modification of said polynucleotides and/or encoded polypeptides has a direct or indirect impact on yield, production, and/or efficiency of production of the fermentation product in said microorganism.
    Type: Grant
    Filed: October 17, 2012
    Date of Patent: August 25, 2015
    Assignee: DSM IP ASSETS B.V.
    Inventors: Marie-Gabrielle Beuzelin-Ollivier, Bastien Chevreux, Manuela Dalluege, Marina Van Gelder, Markus G. Goese, Corina Hauk, Bertus P. Koekman, Connie Lee, Anne F. Mayer, Anja Meury, Nigel J. Mouncey, Dick Schipper, Masako Shinjoh, Christine Toepfer, Adrianus W. H. Vollebregt
  • Patent number: 9090920
    Abstract: Microorganism selected from Gluconobacter, Gluconacetobacter, Acetobacter or Ketogulonicigenium, wherein a gene encoding a polypeptide with the activity of a repressor of L-sorbosone dehydrogenase (SNDH) and L-sorbose dehydrogenase (SDH) is disrupted. The gene has a polynucleotide selected from polynucleotides encoding a polypeptide comprising the amino acid sequence of which is represented by SEQ ID NO:2, polynucleotide comprising the nucleotide sequence according to SEQ ID NO:1, polynucleotides the complementary strand of which hybridizes under stringent conditions to a polynucleotide as defined above and which encodes a polypeptide with the activity of a repressor of SNDH and SDH. The microorganism produces at least 10% more Vitamin C and/or 2-KGA from L-sorbose compared to a microorganism wherein the repressor is intact.
    Type: Grant
    Filed: September 7, 2006
    Date of Patent: July 28, 2015
    Assignee: DSM IP ASSETS B.V.
    Inventors: Bastien Chevreux, Nigel J. Mouncey, Masako Shinjoh
  • Patent number: 9079951
    Abstract: The present invention relates to newly identified genes that encode proteins that are involved in the synthesis of L-ascorbic acid (hereinafter also referred to as Vitamin C). The invention also features polynucleotides comprising the full-length polynucleotide sequences of the novel genes and fragments thereof, the novel polypeptides encoded by the polynucleotides and fragments thereof, as well as their functional equivalents. The present invention also relates to the use of the polynucleotides and polypeptides as biotechnological tools in the production of Vitamin C from microorganisms, whereby a modification of the polynucleotides and/or encoded polypeptides has a direct or indirect impact on yield, production, and/or efficiency of production of the fermentation product in the microorganism. Also included are methods/processes of using the polynucleotides and modified polynucleotide sequences to transform host microorganisms.
    Type: Grant
    Filed: October 15, 2010
    Date of Patent: July 14, 2015
    Assignee: DSM IP ASSETS B.V.
    Inventors: Bastien Chevreux, Corina Hauk, Andrea Muffler, Nigel J. Mouncey, Masako Shinjoh
  • Patent number: 8940503
    Abstract: The present invention relates to microorganisms genetically engineered to increase yield and/or efficiency of biomass production from a carbon source, such as e.g. glucose. Processes for generating such microorganisms are also provided by the present invention. The invention also relates to polynucleotide sequences comprising genes that encode proteins that are involved in the bioconversion of a carbon source such as e.g. glucose into biomass. The invention also features polynucleotides comprising the full-length polynucleotide sequences of the novel genes and fragments thereof, the novel polypeptides encoded by the polynucleotides and fragments thereof, as well as their functional equivalents. Also included are processes of using the polynucleotides and modified polynucleotide sequences to transform host microorganisms leading to a microorganism with reduced carbon source diversion, i.e. higher yield and/or efficiency of biomass production from a carbon source such as e.g. glucose.
    Type: Grant
    Filed: June 17, 2010
    Date of Patent: January 27, 2015
    Assignee: DSM IP Assets B.V.
    Inventor: Masako Shinjoh
  • Patent number: 8771979
    Abstract: The present invention relates to microorganisms genetically engineered to increase yield and/or efficiency of biomass production from a carbon source, such as e.g. glucose. Also included are processes of using the polynucleotides and modified polynucleotide sequences to transform host microorganisms leading to a microorganism with reduced carbon source diversion, i.e. higher yield and/or efficiency of biomass production from a carbon source such as e.g. glucose.
    Type: Grant
    Filed: September 7, 2006
    Date of Patent: July 8, 2014
    Assignee: DSM IP Assets B.V.
    Inventor: Masako Shinjoh
  • Patent number: 8338144
    Abstract: The present invention discloses an isolated polynucleotide molecule derived from a polynucleotide encoding a polypeptide having L-sorbosone dehydrogenase activity comprising a partial nucleotide sequence of at least 20 consecutive nucleotides of SEQ ID NO:1. The present invention further relates to a process for the production of L-ascorbic acid in high yield, in particular a process using resting cells of a microorganism able to convert given carbon sources into vitamin C. The thus obtained vitamin C may be further processed by purification and/or separation steps.
    Type: Grant
    Filed: February 8, 2010
    Date of Patent: December 25, 2012
    Assignee: DSM IP Assets B.V.
    Inventors: Alan Berry, Connie Lee, Anne Fran├žoise Mayer, Masako Shinjoh
  • Patent number: 8318462
    Abstract: The present invention relates to newly identified microorganisms capable of direct production of L-ascorbic acid (hereinafter also referred to as Vitamin C). The invention also relates to polynucleotide sequences comprising genes that encode proteins which are involved in the synthesis of Vitamin C. The invention also features polynucleotides comprising the full length polynucleotide sequences of the novel genes and fragments thereof, the novel polypeptides encoded by the polynucleotides and fragments thereof, as well as their functional equivalents. The present invention also relates to the use of said polynucleotides and polypeptides as biotechnological tools in the production of Vitamin C from microorganisms, whereby a modification of said polynucleotides and/or encoded polypeptides has a direct or indirect impact on yield, production, and/or efficiency of production of the fermentation product in said microorganism.
    Type: Grant
    Filed: August 1, 2007
    Date of Patent: November 27, 2012
    Assignee: DSM IP Assets B.V.
    Inventors: Marie-Gabrielle Beuzelin-Ollivier, Bastian Chevreux, Manuela Dalluege, Marina Van Gelder, Markus G. Goese, Corina Hauk, Bertus P. Koekman, Connie Lee, Anne F. Mayer, Anja Meury, Nigel J. Mouncey, Dick Schipper, Masako Shinjoh, Christine Toepfer, Adrianus W. H. Vollebregt
  • Patent number: 8137940
    Abstract: The present invention relates to microorganisms genetically engineered to increase yield and/or efficiency of biomass production from a carbon source, such as e.g. glucose. Processes for generating such microorganisms are also provided by the present invention. The invention also relates to polynucleotide sequences comprising genes that encode proteins that are involved in the bioconversion of a carbon source such as e.g. glucose into biomass. The invention also features polynucleotides comprising the full-length polynucleotide sequences of the novel genes and fragments thereof, the novel polypeptides encoded by the polynucleotides and fragments thereof, as well as their functional equivalents. Also included are processes of using the polynucleotides and modified polynucleotide sequences to transform host microorganisms leading to a microorganism with reduced carbon source diversion, i.e. higher yield and/or efficiency of biomass production from a carbon source such as e.g. glucose.
    Type: Grant
    Filed: September 7, 2006
    Date of Patent: March 20, 2012
    Assignee: DSM IP Assets B.V.
    Inventor: Masako Shinjoh
  • Publication number: 20120058563
    Abstract: The present invention relates to the production of recombinant microorganisms, in particular of the genus Gluconobacter, for production of 2-keto-L-gulonic acid (2-KGA) and/or L-ascorbic acid (hereinafter also referred to as Vitamin C), wherein the microorganism has been modified to overexpress L-sorbose dehydrogenase (SDH). This overexpression has been achieved by introducing of one or more copies of a polynucleotide encoding SDH into the genome of the host microorganism resulting in enhanced yield, production, and/or efficiency of 2-KGA production and/or Vitamin C compared to a non-modified microorganism. Expression of said one or more extra-copies of sdh is dependent on the integration site. The invention also relates to genetically engineered microorganisms and their use for the production of 2-KGA and/or Vitamin C.
    Type: Application
    Filed: March 4, 2010
    Publication date: March 8, 2012
    Inventors: Tatsuo Hoshino, Nigel John Mouncey, Akiko Shimizu, Masako Shinjoh
  • Patent number: 8053218
    Abstract: The present invention relates to newly identified genes that encode proteins that are involved in the synthesis of L-ascorbic acid (hereinafter also referred to as Vitamin C). The invention also features polynucleotides comprising the full-length polynucleotide sequences of the novel genes and fragments thereof, the novel polypeptides encoded by the polynucleotides and fragments thereof, as well as their functional equivalents. The present invention also relates to the use of the polynucleotides and polypeptides as biotechnological tools in the production of Vitamin C from microorganisms, whereby a modification of the polynucleotides and/or encoded polypeptides has a direct or indirect impact on yield, production, and/or efficiency of production of the fermentation product in the microorganism. Also included are methods/processes of using the polynucleotides and modified polynucleotide sequences to transform host microorganisms.
    Type: Grant
    Filed: February 10, 2006
    Date of Patent: November 8, 2011
    Assignee: DSM IP Assets B.V.
    Inventors: Bastian Chevreux, Anne F. Mayer, Anja Meury, Nigel J. Mouncey, Masako Shinjoh
  • Publication number: 20110250636
    Abstract: The present invention relates to microorganisms genetically engineered to increase yield and/or efficiency of biomass production from a carbon source, such as e.g. glucose. Processes for generating such microorganisms are also provided by the present invention. The invention also relates to polynucleotide sequences comprising genes that encode proteins that are involved in the bioconversion of a carbon source such as e.g. glucose into biomass. The invention also features polynucleotides comprising the full-length polynucleotide sequences of the novel genes and fragments thereof, the novel polypeptides encoded by the polynucleotides and fragments thereof, as well as their functional equivalents. Also included are processes of using the polynucleotides and modified polynucleotide sequences to transform host microorganisms leading to a microorganism with reduced carbon source diversion, i.e. higher yield and/or efficiency of biomass production from a carbon source such as e.g. glucose.
    Type: Application
    Filed: June 17, 2010
    Publication date: October 13, 2011
    Applicant: DSM IP Assets B.V.
    Inventor: Masako Shinjoh
  • Publication number: 20110039310
    Abstract: The present invention relates to newly identified genes that encode proteins that are involved in the synthesis of L-ascorbic acid (hereinafter also referred to as Vitamin C). The invention also features polynucleotides comprising the full-length polynucleotide sequences of the novel genes and fragments thereof, the novel polypeptides encoded by the polynucleotides and fragments thereof, as well as their functional equivalents. The present invention also relates to the use of the polynucleotides and polypeptides as biotechnological tools in the production of Vitamin C from microorganisms, whereby a modification of the polynucleotides and/or encoded polypeptides has a direct or indirect impact on yield, production, and/or efficiency of production of the fermentation product in the microorganism. Also included are methods/processes of using the polynucleotides and modified polynucleotide sequences to transform host microorganisms.
    Type: Application
    Filed: October 15, 2010
    Publication date: February 17, 2011
    Applicant: DSM IP ASSETS B.V.
    Inventors: Bastien CHEVREUX, Corina Hauk, Andrea Muffler, Nigel Mouncey, Masako Shinjoh
  • Patent number: 7842483
    Abstract: The present invention is directed to a novel cytochrome c oxidase complex, genetic materials useful for the preparation of the said complex, such as recombinant polypeptides involved in cytochrome c oxidase complex, recombinant DNA fragments, expression vectors, recombinant organisms and the like. Those novel cytochrome c oxidase complex and genetic materials may be originated from a microorganism having the identifying characteristics of Gluconobacter oxydans DSM 4025. The present invention also provides a method for the preparation of the said novel recombinant cytochrome c oxidase complex and a process for the production of 2-keto-L-gulonic acid (2KGA).
    Type: Grant
    Filed: January 26, 2005
    Date of Patent: November 30, 2010
    Assignee: DSM Nutritional Products, Inc.
    Inventors: Akira Asakura, Tatsuo Hoshino, Masako Shinjoh
  • Publication number: 20100248315
    Abstract: The present invention relates to newly identified microorganisms capable of direct production of L-ascorbic acid (hereinafter also referred to as Vitamin C). The invention also relates to polynucleotide sequences comprising genes that encode proteins which are involved in the synthesis of Vitamin C. The invention also features polynucleotides comprising the full length polynucleotide sequences of the novel genes and fragments thereof, the novel polypeptides encoded by the polynucleotides and fragments thereof, as well as their functional equivalents. The present invention also relates to the use of said polynucleotides and polypeptides as biotechnological tools in the production of Vitamin C from microorganisms, whereby a modification of said polynucleotides and/or encoded polypeptides has a direct or indirect impact on yield, production, and/or efficiency of production of the fermentation product in said microorganism.
    Type: Application
    Filed: August 1, 2007
    Publication date: September 30, 2010
    Inventors: Marie-Gabrielle Beuzelin-Ollivier, Bastian Chevreux, Manuela Dalluegge, Marina Van Gelder, Markus G. Goese, Corina Hauk, Bertus P. Koekman, Connie Lee, Anne F. Mayer, Anja Meury, Nigel J. Mouncey, Dick Schipper, Masako Shinjoh, Christine Toepfer, Adrianus W.H. Vollebregt
  • Publication number: 20100203598
    Abstract: The present invention discloses an isolated polynucleotide molecule derived from a polynucleotide encoding a polypeptide having L-sorbosone dehydrogenase activity comprising a partial nucleotide sequence of at least 20 consecutive nucleotides of SEQ ID NO:1. The present invention further relates to a process for the production of L-ascorbic acid in high yield, in particular a process using resting cells of a microorganism able to convert given carbon sources into vitamin C. The thus obtained vitamin C may be further processed by purification and/or separation steps.
    Type: Application
    Filed: February 8, 2010
    Publication date: August 12, 2010
    Inventors: ALAN BERRY, CONNIE LEE, ANNE FRANCOISE MAYER, MASAKO SHINJOH
  • Publication number: 20100151516
    Abstract: The present invention relates to newly identified genes that encode proteins that are involved in the synthesis of L-ascorbic acid (hereinafter also referred to as Vitamin C). The invention also features polynucleotides comprising the full-length polynucleotide sequences of the novel genes and fragments thereof, the novel polypeptides encoded by the polynucleotides and fragments thereof, as well as their functional equivalents. The present invention also relates to the use of said polynucleotides and polypeptides as biotechnological tools in the production of Vitamin C from microorganisms, whereby a modification of said polynucleotides and/or encoded polypeptides has a direct or indirect impact on yield, production, and/or efficiency of production of the fermentation product in said microorganism. Also included are methods/processes of using the polynucleotides and modified polynucleotide sequences to transform host microorganisms.
    Type: Application
    Filed: February 10, 2006
    Publication date: June 17, 2010
    Inventors: Bastian Chevreux, Ann Mayer, Masako Shinjoh
  • Patent number: 7700723
    Abstract: The present invention discloses an isolated polynucleotide molecule derived from a polynucleotide encoding a polypeptide having L-sorbosone dehydrogenase activity comprising a partial nucleotide sequence of at least 20 consecutive nucleotides of SEQ ID NO:1. The present invention further relates to a process for the production of L-ascorbic acid in high yield, in particular a process using resting cells of a microorganism able to convert given carbon sources into vitamin C. The thus obtained vitamin C may be further processed by purification and/or separation steps.
    Type: Grant
    Filed: August 16, 2004
    Date of Patent: April 20, 2010
    Assignee: DSM IP Assets B.V.
    Inventors: Alan Berry, Connie Lee, Anne Fran├žoise Mayer, Masako Shinjoh
  • Publication number: 20090269852
    Abstract: The present invention relates to microorganisms genetically engineered to increase yield and/or efficiency of biomass production from a carbon source, such as e.g. glucose. Processes for generating such microorganisms are also provided by the present invention. The invention also relates to polynucleotide sequences comprising genes that encode proteins that are involved in the bioconversion of a carbon source such as e.g. glucose into biomass. The invention also features polynucleotides comprising the full-length polynucleotide sequences of the novel genes and fragments thereof, the novel polypeptides encoded by the polynucleotides and fragments thereof, as well as their functional equivalents. Also included are processes of using the polynucleotides and modified polynucleotide sequences to transform host microorganisms leading to a microorganism with reduced carbon source diversion, i.e. higher yield and/or efficiency of biomass production from a carbon source such as e.g. glucose.
    Type: Application
    Filed: September 7, 2006
    Publication date: October 29, 2009
    Inventor: Masako Shinjoh
  • Publication number: 20090215135
    Abstract: The present invention relates to newly identified genes that encode proteins that are involved in the synthesis of L-ascorbic acid (hereinafter also referred to as Vitamin C). The invention also features polynucleotides comprising the full-length polynucleotide sequences of the novel genes and fragments thereof, the novel polypeptides encoded by the polynucleotides and fragments thereof, as well as their functional equivalents. The present invention also relates to the use of said polynucleotides and polypeptides as biotechnological tools in the production of Vitamin C from microorganisms, whereby a modification of said polynucleotides and/or encoded polypeptides has a direct or indirect impact on yield, production, and/or efficiency of production of the fermentation product in said microorganism. Also included are methods/processes of using the polynucleotides and modified polynucleotide sequences to transform host microorganisms.
    Type: Application
    Filed: February 10, 2006
    Publication date: August 27, 2009
    Applicant: DSM IP ASSETS B.V.
    Inventors: Marie-Gabrielle Beuzelin-Ollivier, Bastian Chevreux, Manuela Dalluege, Markus G. Goese, Connie Lee, Anja Meury, Nigel J. Mouncey, Masako Shinjoh