Abstract: The present invention has for its object to provide an inspection chip using a metasurface for detection of biomolecules by a fluorescence detection method. The inspection chip comprises a first substrate having a metasurface, and a second substrate positioned in opposition to the first substrate and having a microchannel, as shown in FIG. 1. The metasurface includes a gap for efficient immobilization of the biomolecules to be detected, and induces or develops fluorescence enhancement in a region including a wavelength range of fluorescence emitted by the biomolecules to be detected. The second substrate is formed of a material transparent to visible light or near infrared light, and the fluorescence resonates between the first substrate and second substrate.