Abstract: There are provided hyperthermostable proteases having an amino acid sequences represented by SEQ ID Nos. 1, 3 and 5 of the Sequence Listing or functional equivalents thereof and hyperthermostable protease genes encoding those hyperthermostable protease. There is also disclosed a process for preparation of a hyperthermostable protease by culturing a transformant containing the gene.

Abstract: Regulatory elements of the 5?UTR of cold-shock inducible genes that prolong the expression of cold-shock inducible genes under conditions that elicit the cold-shock response in a bacterium, repress the expression of the cold-shock inducible genes under physiological conditions, or enhance the translation of cold-shock inducible genes under conditions that elicit a cold-shock response in bacteria; vectors incorporating these elements; and methods for expressing proteins.

Abstract: There are provided hyperthermostable proteases having an amino acid sequences represented by SEQ ID Nos. 1, 3 and 5 of the Sequence Listing or functional equivalents thereof and hyperthermostable protease genes encoding those hyperthermostable protease. There is also disclosed a process for preparation of a hyperthermostable protease by culturing a transformant containing the gene.

Abstract: There are provided hyperthermostable proteases having an amino acid sequences represented by SEQ ID NOs: 1, 3 and 5 of the Sequence Listing or functional equivalents thereof and hyperthermostable protease genes encoding those hyperthermostable protease. There is also disclosed a process for preparation of a hyperthermostable protease by culturing a transformant containing the gene.

Abstract: There are provided hyperthermostable proteases having an amino acid sequences represented by SEQ ID Nos. 1, 3 and 5 of the Sequence Listing or functional equivalents thereof and hyperthermostable protease genes encoding those hyperthermostable protease. There is also disclosed a process for preparation of a hyperthermostable protease by culturing a transformant containing the gene.

Abstract: A method of inhibiting the translation of bacterial mRNA is disclosed. The method comprises overexpressing in a bacterium an mRNA which contains a sequence which is complementary to the anti-downstream box region of the 16S rRNA. RNA and DNA constructs for the overexpression of the mRNA of the invention are disclosed. Further, there are disclosed isolated DNA constructs that direct the prolonged expression of a heterologous gene in a cold-shocked bacterium at reduced temperature. The construct can comprise a promoter region of a cold-shocked inducible gene. The replication vehicle comprising such DNA constructs and a method for overexpressing a heterologous gene in a bacterium transformed with such a replication vehicle are also disclosed.

Abstract: There are provided hyperthermostable proteases having an amino acid sequences represented by SEQ ID NOs: 1, 3 and 5 of the Sequence Listing or functional equivalents thereof and hyperthermostable protease genes encoding those hyperthermostable protease. There is also disclosed a process for preparation of a hyperthermostable protease by culturing a transformant containing the gene.

Abstract: There are provided hyperthermostable proteases having an amino acid sequences represented by SEQ ID Nos. 1, 3 and 5 of the Sequence Listing or functional equivalents thereof and hyperthermostable protease genes encoding those hyperthermostable protease. There is also disclosed a process for preparation of a hyperthermostable protease by culturing a transformant containing the gene.

Abstract: A method of inhibiting the translation of bacterial mRNA is disclosed, which method comprises overexpressing in a bacterium an mRNA which contains a sequence which is complementary to the anti-downstream box region of the 16S rRNA. RNA and DNA constructs for the overexpression of the mRNA of the invention are disclosed.

Abstract: An isolated DNA having a sequence encoding a polypeptide possessing lacto-N-biosidase activity or functionally equivalent variants thereof; and a method for producing a polypeptide possessing lacto-N-biosidase activity or functionally equivalent variants thereof using the isolated DNA by recombinant DNA technology.

Abstract: There is disclosed a hyperthermostable protease gene originating in Pyrococcus furiosus, in particular, a hyperthermostable protease gene encoding the amino acid sequence represented by the SEQ ID NO 1 in the Sequence Listing or a part thereof which retains the activity of the hyperthermostable protease. There is also disclosed a process for producing the protease by culturing a transformant transformed with a plasmid into which the above gene has been inserted.

Abstract: An almond N-glycosidase gene and genes associated therewith are described. Vectors integrated such genes therein, recombinant microorganisms transformed with said vectors, and a process for preparing the almond N-glycosidase using said recombinant microorganisms are also described.

Abstract: An isolated .alpha.-1,3/4-fucosidase gene is provided which codes for an .alpha.-1,3/4-fucosidase enzyme. The isolated gene codes for the fucosidase enzyme having the amino acid sequence shown in SEQ ID No. 1. The gene may have the DNA sequence shown in SEQ ID No. 2. A process for producing the fucosidase enzyme is also provided, which comprises cultivating a transformant into which a recombinant plasmid containing the .alpha.-1,3/4-fucosidase gene according to the invention has been introduced to produce the fucosidase enzyme encoded thereby, and harvesting the fucosidase enzyme from the culture.

Abstract: There is provided an isolated gene from Arthrobacter oxidans encoding L-fucose dehydrogenase. Also provided are a process for producing L-fucose dehydrogenase by culturing E. coli containing a plasmid that carries the gene encoding the enzyme.