Abstract: A process is described for cultivating thermophilic bacteria of the genus Bacillus having the ability to produce acetate kinase, wherein said cultivation is carried out continuously at a dilution rate (D) in the range from about 0.9 .mu..sub.max to 1.0 .mu..sub.max, wherein D is the dilution rate (1/hr), and .mu..sub.max is the maximum specific growth rate (1/hr), to thereby produce bacterial cells having a high content of acetate kinase.

Abstract: A reagent composition for determining blood triglycerides comprising a color former and a thermostable lipoprotein lipase obtained from Streptomyces 7825 (FERM P-9983, FERM BP-2489) is presented.

Abstract: The present invention is directed toward a thermostable lipoprotein lipase capable of hydrolyzing triglycerides in lipoproteins to glycerol and fatty acids, a process for producing the thermostable lipoprotein lipase, and a blood triglyceride determining reagent containing the thermostable lipoprotein lipase. The thermostable lipoprotein lipase exhibits about 100% retention of the hydrolyzing activity when treated in a buffer having a pH of from bout 4 to 7 at about 60.degree. C. for about 15 minutes and a glycerol forming activity/fatty acid forming activity ratio of at least about 15%. The process comprises cultivating a thermophilic actinomycetes, particularly Streptomyces 7825 (FERM P-9983, FERM BP-2489 is cultivated to produce a thermostable lipoprotein lipase.

Abstract: The present invention is directed toward a thermostable lipoprotein lipase capable of hydrolyzing triglycerides in lipoproteins to glycerol and fatty acids, a process for producing the thermostable lipoprotein lipase, and a blood triglyceride determining reagent containing the thermostable lipoprotein lipase. The thermostable lipoprotein lipase exhibits about 100% retention of the hydrolyzing activity when treated in a buffer having a pH of from about 4 to 7 at about 60.degree. C. for about 15 minutes and a glycerol forming activity/fatty acid forming activity ratio of at least about 15%. The process comprises cultivating a thermophilic actinomycetes, particularly Streptomyces 7825 (FERM P-9983, FERM BP-2489 and recovering lipoprotein lipase from the culture.

Abstract: Disclosed is a reagent for measuring amylase activity in body fluids by cleaving an oligosaccharide having a defined chain length with amylase in body fluids to produce a glucose and measuring said glucose, characterized in that the reagent is divided to two portions, the first portion comprises an enzyme for converting a glucose and/or maltose naturally present in body fluids to glucose-6-phosphate, phosphoglucose isomerase, phosphofructokinase and adenosine-5'-triphosphate, and the second portion comprises said oligosaccharide being used as a substrate and a phosphoric acid ester of saccharides and/or saccharic acids; and a method for measuring amylase activity in body fluids comprising;eliminating glucose and/or maltose naturally present in body fluids with a first reagent comprising an enzyme for converting the glucose and/or maltose to glucose-6-phosphate, phosphoglucose isomerase, phosphofructokinase and adenosine-5'-triphosphate,adding a second reagent comprising a oligosaccharide substrate and a phosp

Abstract: An acyl-CoA synthetase is disclosed having the following characteristics:(a) Reaction:Capable of acting on saturated or unsaturated lower to higher fatty acids to produce a CoA derivative thereof (Acyl-CoA), adenosine monophosphate and pyrophosporic acid, in the presence of adenosine triphosphate and coenzyme A;(b) Stability:Having a residual activity of not less than about 50% after being incubated in a buffer (pH about 7.5 for about 15 minutes at a temperature of about 55.degree. C.; and(c) Optimum reaction temperature:Having an optimum reaction temperature in the range of from 50.degree. to 60.degree. C.Also disclosed is Psuedomonas UKSW-3733 strain which is capable of producing the acyl-CoA synthetase, which has an optimum temperature for growth in the range of from 45.degree. to 55.degree. C. and is incapable of assimilating carbonates.

Abstract: A reagent system for assaying creatine kinase is disclosed. The reagent system consisting essentially of a first reagent comprising glucose-6-phosphate dehydrogenase, .beta.-nicotinamideadenine dinucleotide (phosphate), and adenosine diphosphate, and a second reagent comprising creatine phosphate, said second reagent being maintained at a pH of from 7.5 to 10, and at least one of said first reagent and said second reagent containing glucokinase and glucose. The creatine kinase-assaying reagent exhibits remarkably improved stability in a dissolved state so that it can be prepared in large quantities and can be conventionally utilized to cope with urgent clinical examinations.

Abstract: Strain UK 788 (FERM-P No. 5141) that belongs to Bacillus stearothermophilus, the cell of which is longer than about 10 microns and which permits easier release of intracellular components than a type culture Bacillus stearothermophilus, IAM 11001 is disclosed. Also, a process for producing a useful enzyme selected from the group consisting of a heat-resistant polynucleotide phosphorylase, heat-resistant maleate dehydrogenase, heat-resistant glucokinase, heat-resistant glucose-6-phosphate dehydrogenase and heat-resistant pyruvate kinase by culturing such UK 788 and recovering the desired enzyme from the culture is disclosed. Since the cell of the strain UK 788 is easy to settle and its membrane is also easy to break, the useful enzymes mentioned above can be efficiently produced on an industrial scale.