Patents by Inventor Masao Kageyama

Masao Kageyama has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Patent number: 5610045
    Abstract: A process is described for cultivating thermophilic bacteria of the genus Bacillus having the ability to produce acetate kinase, wherein said cultivation is carried out continuously at a dilution rate (D) in the range from about 0.9 .mu..sub.max to 1.0 .mu..sub.max, wherein D is the dilution rate (1/hr), and .mu..sub.max is the maximum specific growth rate (1/hr), to thereby produce bacterial cells having a high content of acetate kinase.
    Type: Grant
    Filed: June 2, 1995
    Date of Patent: March 11, 1997
    Assignee: Unitika Ltd.
    Inventors: Masao Kageyama, Toyohiko Suga, Kenzo Motosugi, Hiroshi Nakajima
  • Patent number: 5244798
    Abstract: A reagent composition for determining blood triglycerides comprising a color former and a thermostable lipoprotein lipase obtained from Streptomyces 7825 (FERM P-9983, FERM BP-2489) is presented.
    Type: Grant
    Filed: August 17, 1992
    Date of Patent: September 14, 1993
    Assignee: Unitika Ltd.
    Inventors: Akira Takeda, Tomoko Kamei, Masao Kageyama, Kenzo Motosugi
  • Patent number: 5232846
    Abstract: The present invention is directed toward a thermostable lipoprotein lipase capable of hydrolyzing triglycerides in lipoproteins to glycerol and fatty acids, a process for producing the thermostable lipoprotein lipase, and a blood triglyceride determining reagent containing the thermostable lipoprotein lipase. The thermostable lipoprotein lipase exhibits about 100% retention of the hydrolyzing activity when treated in a buffer having a pH of from bout 4 to 7 at about 60.degree. C. for about 15 minutes and a glycerol forming activity/fatty acid forming activity ratio of at least about 15%. The process comprises cultivating a thermophilic actinomycetes, particularly Streptomyces 7825 (FERM P-9983, FERM BP-2489 is cultivated to produce a thermostable lipoprotein lipase.
    Type: Grant
    Filed: September 3, 1992
    Date of Patent: August 3, 1993
    Assignee: Unitika Ltd.
    Inventors: Akira Takeda, Tomoko Kamei, Masao Kageyama, Kenzo Motosugi
  • Patent number: 5173417
    Abstract: The present invention is directed toward a thermostable lipoprotein lipase capable of hydrolyzing triglycerides in lipoproteins to glycerol and fatty acids, a process for producing the thermostable lipoprotein lipase, and a blood triglyceride determining reagent containing the thermostable lipoprotein lipase. The thermostable lipoprotein lipase exhibits about 100% retention of the hydrolyzing activity when treated in a buffer having a pH of from about 4 to 7 at about 60.degree. C. for about 15 minutes and a glycerol forming activity/fatty acid forming activity ratio of at least about 15%. The process comprises cultivating a thermophilic actinomycetes, particularly Streptomyces 7825 (FERM P-9983, FERM BP-2489 and recovering lipoprotein lipase from the culture.
    Type: Grant
    Filed: August 9, 1989
    Date of Patent: December 22, 1992
    Assignee: Unitika Ltd.
    Inventors: Akira Takeda, Tomoko Kamei, Masao Kageyama, Kenzo Motosugi
  • Patent number: 4812398
    Abstract: Disclosed is a reagent for measuring amylase activity in body fluids by cleaving an oligosaccharide having a defined chain length with amylase in body fluids to produce a glucose and measuring said glucose, characterized in that the reagent is divided to two portions, the first portion comprises an enzyme for converting a glucose and/or maltose naturally present in body fluids to glucose-6-phosphate, phosphoglucose isomerase, phosphofructokinase and adenosine-5'-triphosphate, and the second portion comprises said oligosaccharide being used as a substrate and a phosphoric acid ester of saccharides and/or saccharic acids; and a method for measuring amylase activity in body fluids comprising;eliminating glucose and/or maltose naturally present in body fluids with a first reagent comprising an enzyme for converting the glucose and/or maltose to glucose-6-phosphate, phosphoglucose isomerase, phosphofructokinase and adenosine-5'-triphosphate,adding a second reagent comprising a oligosaccharide substrate and a phosp
    Type: Grant
    Filed: May 9, 1986
    Date of Patent: March 14, 1989
    Assignee: Unitika Ltd.
    Inventors: Hitoshi Kondo, Masao Kageyama
  • Patent number: 4794084
    Abstract: An acyl-CoA synthetase is disclosed having the following characteristics:(a) Reaction:Capable of acting on saturated or unsaturated lower to higher fatty acids to produce a CoA derivative thereof (Acyl-CoA), adenosine monophosphate and pyrophosporic acid, in the presence of adenosine triphosphate and coenzyme A;(b) Stability:Having a residual activity of not less than about 50% after being incubated in a buffer (pH about 7.5 for about 15 minutes at a temperature of about 55.degree. C.; and(c) Optimum reaction temperature:Having an optimum reaction temperature in the range of from 50.degree. to 60.degree. C.Also disclosed is Psuedomonas UKSW-3733 strain which is capable of producing the acyl-CoA synthetase, which has an optimum temperature for growth in the range of from 45.degree. to 55.degree. C. and is incapable of assimilating carbonates.
    Type: Grant
    Filed: February 5, 1987
    Date of Patent: December 27, 1988
    Assignee: Unitika Ltd.
    Inventors: Mitsuo Watanabe, Hiromi Sato, Tomoko Kamei, Masao Kageyama
  • Patent number: 4740458
    Abstract: A reagent system for assaying creatine kinase is disclosed. The reagent system consisting essentially of a first reagent comprising glucose-6-phosphate dehydrogenase, .beta.-nicotinamideadenine dinucleotide (phosphate), and adenosine diphosphate, and a second reagent comprising creatine phosphate, said second reagent being maintained at a pH of from 7.5 to 10, and at least one of said first reagent and said second reagent containing glucokinase and glucose. The creatine kinase-assaying reagent exhibits remarkably improved stability in a dissolved state so that it can be prepared in large quantities and can be conventionally utilized to cope with urgent clinical examinations.
    Type: Grant
    Filed: October 4, 1985
    Date of Patent: April 26, 1988
    Assignee: Unitika Ltd.
    Inventors: Hitoshi Kondo, Masao Kageyama, Kosuke Tomita
  • Patent number: 4331762
    Abstract: Strain UK 788 (FERM-P No. 5141) that belongs to Bacillus stearothermophilus, the cell of which is longer than about 10 microns and which permits easier release of intracellular components than a type culture Bacillus stearothermophilus, IAM 11001 is disclosed. Also, a process for producing a useful enzyme selected from the group consisting of a heat-resistant polynucleotide phosphorylase, heat-resistant maleate dehydrogenase, heat-resistant glucokinase, heat-resistant glucose-6-phosphate dehydrogenase and heat-resistant pyruvate kinase by culturing such UK 788 and recovering the desired enzyme from the culture is disclosed. Since the cell of the strain UK 788 is easy to settle and its membrane is also easy to break, the useful enzymes mentioned above can be efficiently produced on an industrial scale.
    Type: Grant
    Filed: November 21, 1980
    Date of Patent: May 25, 1982
    Assignee: Unitika Ltd.
    Inventors: Hiroshi Nakajima, Kazuhiko Nagata, Masao Kageyama, Toyohiko Suga, Tadao Suzuki, Kenzo Motosugi