Abstract: A method includes detecting a nucleotide sequence in genomic DNA obtained from a sample; and evaluating a content of mesenchymal stem cells in the sample. The nucleotide sequence comprises at least one CpG site selected from the group consisting of: a CpG site consisting of C at position 157 and G at position 158 of SEQ ID NO: 1; and a CpG site complementary to the CpG site of SEQ ID NO: 1 in a nucleotide sequence complementary to the nucleotide sequence of SEQ ID NO: 1. The sample is an adipose tissue or a cell-containing fraction obtained from an adipose tissue.

Abstract: A method includes detecting a nucleotide sequence in genomic DNA obtained from a sample; and evaluating a content of mesenchymal stem cells in the sample. The nucleotide sequence comprises at least one CpG site selected from the group consisting of: a CpG site consisting of C at position 157 and G at position 158 of SEQ ID NO: 1; and a CpG site complementary to the CpG site of SEQ ID NO: 1 in a nucleotide sequence complementary to the nucleotide sequence of SEQ ID NO: 1. The sample is an adipose tissue or a cell-containing fraction obtained from an adipose tissue.

Abstract: The present invention relates to a separation system and a separation material for easily and rapidly separating a red blood cell-rich fraction, a white blood cell-rich fraction, and/or a platelet-rich fraction from a biological fluid containing these blood cell components without the necessity of performing centrifugation. The separation of a red blood cell-rich fraction, a white blood cell-rich fraction, and/or a platelet-rich fraction from a biological fluid can be accomplished by capturing white blood cells and platelets on a blood cell separation material by contacting the biological fluid with the blood cell separation material, thereby providing a red blood cell-rich fraction; and recovering a white blood cell-rich fraction by separating white blood cells captured from the resulting blood cell separation material using a separation solution.

Abstract: The present invention aims to provide a leukocyte depletion filter that has both higher leukocyte removal performance and higher blood permeability than conventional filters. The leukocyte depletion filter according to the present invention includes a filter material having an average fiber diameter of 0.30 to 1.60 ?m and a coefficient of variation of fiber diameter of at least 0.40 but not more than 1.20.

Abstract: It is an object to achieve a closed system that has a simple structure without resorting to large scale equipment and that sequentially carries out all of the step of separating useful cells, the step of culturing the useful cells separated in the separation step, and the step of washing and concentrating the cells cultured in the culturing step and to enable the preparation of useful cells having high safety and quality, while improving operability.

Abstract: The present invention aims to provide a leukocyte depletion filter that has both higher leukocyte removal performance and higher blood permeability than conventional filters. The leukocyte depletion filter according to the present invention includes a filter material having an average fiber diameter of 0.30 to 1.60 ?m and a coefficient of variation of fiber diameter of at least 0.40 but not more than 1.20.

Abstract: It is an object to achieve a closed system that has a simple structure without resorting to large scale equipment and that sequentially carries out all of the step of separating useful cells, the step of culturing the useful cells separated in the separation step, and the step of washing and concentrating the cells cultured in the culturing step and to enable the preparation of useful cells having high safety and quality, while improving operability.

Abstract: The present invention has its object to provide a direct hemoperfusion adsorber packed with an adsorbent made by using a water-insoluble carrier having a relatively readily available particle size distribution, with water-insoluble microparticles having been removed therefrom, without needing any special granulation apparatus or coating apparatus, and method of obtaining a direct hemoperfusion adsorbent having water-insoluble microparticles removed therefrom.

Abstract: The present invention relates to a separation system and a separation material for easily and rapidly separating a red blood cell-rich fraction, a white blood cell-rich fraction, and/or a platelet-rich fraction from a biological fluid containing these blood cell components without the necessity of performing centrifugation. The separation of a red blood cell-rich fraction, a white blood cell-rich fraction, and/or a platelet-rich fraction from a biological fluid can be accomplished by capturing white blood cells and platelets on a blood cell separation material by contacting the biological fluid with the blood cell separation material, thereby providing a red blood cell-rich fraction; and recovering a white blood cell-rich fraction by separating white blood cells captured from the resulting blood cell separation material using a separation solution.

Abstract: The present invention has its object to provide a direct hemoperfusion adsorber packed with an adsorbent made by using a water-insoluble carrier having a relatively readily available particle size distribution, with water-insoluble microparticles having been removed therefrom, without needing any special granulation apparatus or coating apparatus, and method of obtaining a direct hemoperfusion adsorbent having water-insoluble microparticles removed therefrom.

Abstract: A safe and practically useful body fluid processor whereby blood taken out from a patient can be directly processed, characterized by having a favorable passage of the blood cell and showing an extremely low risk of the generation and leakage of microparticles. Namely, a body fluid processor enabling direct hemoperfusion wherein the ratio of sedimented particles to the volume of the space in which the particles for processing body fluid are to be packed is 100% or less and the space in which the particles for processing body fluid are to be packed in the body fluid processor is filled with the particles for processing body fluid and a filling liquid at a ratio of 95% or more but not more than 100%.

Abstract: An object of the present invention is to provide a method of modifying a blood contact material capable of reducing granulocyte activating action of the blood contact material in medical equipment used for parts which get contact with the blood. The present invention provides a method of modifying blood contact material characterized by granulocyte activating action when in contact with blood,the modifying blood contact material is considerably reduced compared with an original blood contact material by immobilizing a tryptophan derivative and a polyanionic compound to the blood contact material. Examples of the tryptophan derivative include tryptophan. Further, examples of the polyanionic compound include dextran sulfate.

Abstract: A device for body fluid purification, comprising a container having an inlet and an outlet for a fluid and a means for preventing an adsorbent from flowing out of the container and an adsorbent charged in the container, the adsorbent comprising a spherical hydrogel, the spherical hydrogel having an average particle size in a range of from about 460 to 600 ?m and having a substantially uniform particle size and comprising an epoxidated cellulose having hexadecylamine immobilized thereon in an amount of 100 to 300 ?mol per gram of dry weight of said epoxidated cellulose, and water, wherein the weight ratio of said epoxidated cellulose having hexadecylamine immobilized thereon to the water is 2:8.

Abstract: The present invention provides an adsorbent, an adsorption method, and an adsorber for efficiently adsorbing low-density lipoproteins and fibrinogen directly from a body fluid, particularly whole blood, to decrease the concentrations of these components in the body fluid with minimizing losses of useful substances such as HDL and albumin. The adsorbent includes a tryptophan derivative and a polyanionic compound which are immobilized on a water-insoluble porous carrier, wherein the amount of the immobilized polyanionic compound is 0.10 ?mol to 1.5 ?mol per milliliter of wet volume of the adsorbent, and the molar ratio of the amount of the immobilized tryptophan derivative to the amount of the immobilized polyanionic compound is 1 to 70. The adsorbent is capable of whole blood treatment for safely and efficiently adsorbing low-density lipoproteins and fibrinogen.

Abstract: A device for body fluid purification, comprising a container having an inlet and an outlet for a fluid and a means for preventing an adsorbent from flowing out of the container and an adsorbent charged in the container, the adsorbent comprising a spherical hydrogel, the spherical hydrogel having an average particle size in a range of from about 460 to 600 ?m and having a substantially uniform particle size and comprising an epoxidated cellulose having hexadecylamine immobilized thereon in an amount of 100 to 300 ?mol per gram of dry weight of said epoxidated cellulose, and water, wherein the weight ratio of said epoxidated cellulose having hexadecylamine immobilized thereon to the water is 2:8.

Abstract: A device for body fluid purification, comprising a container having an inlet and an outlet for a fluid and a means for preventing an adsorbent from flowing out of the container and an adsorbent charged in the container, the adsorbent comprising a spherical hydrogel, the spherical hydrogel having an average particle size in a range of from about 460 to 600 ?m and having a substantially uniform particle size and comprising an epoxidated cellulose having hexadecylamine immobilized thereon in an amount of 100 to 300 ?mol per gram of dry weight of said epoxidated cellulose, and water, wherein the weight ratio of said epoxidated cellulose having hexadecylamine immobilized thereon to the water is 2:8.

Abstract: A device for body fluid purification, comprising a container having an inlet and an outlet for a fluid and a means for preventing an adsorbent from flowing out of the container and an adsorbent charged in the container, the adsorbent comprising a spherical hydrogel, the spherical hydrogel having an average particle size in a range of from about 460 to 600 &mgr;m and having a substantially uniform particle size and comprising an epoxidated cellulose having hexadecylamine immobilized thereon in an amount of 100 to 300 &mgr;mol per gram of dry weight of said epoxidated cellulose, and water, wherein the weight ratio of said epoxidated cellulose having hexadecylamine immobilized thereon to the water is 2:8.

Abstract: A safe and practically useful body fluid processor whereby blood taken out from a patient can be directly processed, characterized by having a favorable passage of the blood cell and showing an extremely low risk of the generation and leakage of microparticles. Namely, a body fluid processor enabling direct hemoperfusion wherein the ratio of sedimented particles to the volume of the space in which the particles for processing body fluid are to be packed is 100% or less and the space in which the particles for processing body fluid are to be packed in the body fluid processor is filled with the particles for processing body fluid and a filling liquid at a ratio of 95% or more but not more than 100%.