Abstract: A cell culture system for dividing and subculturing cell aggregates is constituted by connecting a first container 10 and a divider 20. An outlet of a divider is connected to the first container. A mesh structure 22 for dividing a cell aggregate is provided in a conduit for dividing in the divider 20, and a cell aggregate is divided while passing through the mesh structure. The divided cell aggregate is sent to the first container 10 without contacting the outside air in a preferred embodiment, where further culture is performed. This makes it possible to divide the cell aggregate and perform suspension culture while maintaining the closed system. The division and suspension culture of cell aggregates can be repeated by sending the cultured cell aggregates to a divider of its own cell culture system or a divider of a cell culture system prepared separately.

Abstract: The present invention provides a cell culture container for long term non-adherent cell culture which comprises having a coating containing a copolymer which is a copolymer having a recurring unit which contains a group represented by the following formula (a), a recurring unit which contains a group represented by the following formula (b), and a recurring unit which contains a group represented by the following formula (c), and a molar ratio of the recurring unit which contains a group represented by the formula (c) being 1 to 50 mol % based on the whole copolymer, provided with at least a part of a surface thereof [wherein, Ua1, Ua2, Ub1, Ub2 and Ub3, Rc, and An? are as defined in the specification and Claims.