Patents by Inventor Masataka Kinjo

Masataka Kinjo has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Publication number: 20210333287
    Abstract: Disclosed is a method for acquiring information of a target polypeptide, comprising: acquiring a diffusion time of a fluorescently labeled target polypeptide and a diffusion time of each of a plurality of fluorescently labeled reference polypeptides by fluorescence correlation spectroscopy or fluorescence cross-correlation spectroscopy, and acquiring information on size of the fluorescently labeled target polypeptide from the diffusion time of the fluorescently labeled target polypeptide with reference to the diffusion times of the plurality of fluorescently labeled reference polypeptides, wherein information on size of each of the plurality of fluorescently labeled reference polypeptides is known, and the sizes of the plurality of reference polypeptides are different from each other.
    Type: Application
    Filed: April 27, 2021
    Publication date: October 28, 2021
    Applicants: NATIONAL UNIVERSITY CORPORATION HOKKAIDO UNIVERSITY, SYSMEX CORPORATION
    Inventors: Masataka KINJO, Akshay GANGULY, Lausonia RAMASWAMY
  • Patent number: 10209504
    Abstract: Provided is a light detecting device including: a light path branching unit that branches a single detection light path of fluorescence from a specimen, into a plurality of branched light paths; a plurality of light detectors that are provided to the respective branched light paths branched by the light path branching unit and that include an SSPD or Geiger mode APD to detect the fluorescence; and a signal adder that generates a single image signal in accordance with the detection signals outputted from the plurality of light detectors.
    Type: Grant
    Filed: March 27, 2018
    Date of Patent: February 19, 2019
    Assignees: OLYMPUS CORPORATION, NATIONAL INSTITUTE OF INFORMATION AND COMMUNICATIONS TECHNOLOGY, OSAKA UNIVERSITY, NATIONAL UNIVERSITY CORPORATION HOKKAIDO UNIVERSITY
    Inventors: Hirokazu Kubo, Shigehito Miki, Hirotaka Terai, Taro Yamashita, Tokuko Haraguchi, Yasushi Hiraoka, Masataka Kinjo
  • Publication number: 20180284413
    Abstract: Provided is a light detecting device including: a light path branching unit that branches a single detection light path of fluorescence from a specimen, into a plurality of branched light paths; a plurality of light detectors that are provided to the respective branched light paths branched by the light path branching unit and that include an SSPD or Geiger mode APD to detect the fluorescence; and a signal adder that generates a single image signal in accordance with the detection signals outputted from the plurality of light detectors.
    Type: Application
    Filed: March 27, 2018
    Publication date: October 4, 2018
    Applicants: OLYMPUS CORPORATION, NATIONAL INSTITUTE OF INFORMATION AND COMMUNICATIONS TECHNOLOGY, OSAKA UNIVERSITY, NATIONAL UNIVERSITY CORPORATION HOKKAIDO UNIVERSITY
    Inventors: Hirokazu KUBO, Shigehito MIKI, Hirotaka TERAI, Taro YAMASHITA, Tokuko HARAGUCHI, Yasushi HIRAOKA, Masataka KINJO
  • Patent number: 8420781
    Abstract: An object of the present invention is to provide a red or orange fluorescent protein, which is characterized in that the difference (stokes shift) between an excitation peak value (wavelength of maximum absorption) and a fluorescence peak value (wavelength of maximum fluorescence) is greatened, so that the maximum fluorescence can be obtained by the maximum excitation. The present invention provides a novel fluorescent protein monomerized by introducing a mutation into a florescent protein derived from Fungia sp., and a novel chromoprotein and fluorescent protein derived from Montipora. sp.
    Type: Grant
    Filed: August 2, 2011
    Date of Patent: April 16, 2013
    Assignees: Riken, Medical & Biological Laboratories Co., Ltd.
    Inventors: Atsushi Miyawaki, Takako Kogure, Hiroshi Hama, Masataka Kinjo, Kenta Saito, Satoshi Karasawa, Toshio Araki
  • Publication number: 20120034643
    Abstract: An object of the present invention is to provide a red or orange fluorescent protein, which is characterized in that the difference (stokes shift) between an excitation peak value (wavelength of maximum absorption) and a fluorescence peak value (wavelength of maximum fluorescence) is greatened, so that the maximum fluorescence can be obtained by the maximum excitation. The present invention provides a novel fluorescent protein monomerized by introducing a mutation into a florescent protein derived from Fungia sp., and a novel chromoprotein and fluorescent protein derived from Montipora. sp.
    Type: Application
    Filed: August 2, 2011
    Publication date: February 9, 2012
    Applicants: MEDICAL & BIOLOGICAL LABORATORIES CO., LTD., RIKEN
    Inventors: Atsushi MIYAWAKI, Takako KOGURE, Hiroshi HAMA, Masataka KINJO, Kenta SAITO, Satoshi KARASAWA, Toshio ARAKI
  • Patent number: 8017746
    Abstract: An object of the present invention is to provide a red or orange fluorescent protein, which is characterized in that the difference (stokes shift) between an excitation peak value (wavelength of maximum absorption) and a fluorescence peak value (wavelength of maximum fluorescence) is greatened, so that the maximum fluorescence can be obtained by the maximum excitation. The present invention provides a novel fluorescent protein monomerized by introducing a mutation into a florescent protein derived from Fungia sp., and a novel chromoprotein and fluorescent protein derived from Montipora. sp.
    Type: Grant
    Filed: December 3, 2004
    Date of Patent: September 13, 2011
    Assignees: Riken, Medical & Biological Laboratories Co., Ltd.
    Inventors: Atsushi Miyawaki, Takako Kogure, Hiroshi Hama, Masataka Kinjo, Kenta Saito, Satoshi Karasawa, Toshio Araki
  • Publication number: 20090253217
    Abstract: The present invention is to provide a method of quickly detecting an antigen at an arbitrary concentration in an antigen sample, without a multi-stage examination of the concentration ratio between a detection reagent and an antigen to be detected, particularly when the concentration of the antigen in the sample is unknown, in the method of detecting an antigen using fluorescence correlation spectroscopy (FCS) or fluorescence cross-correlation spectroscopy (FCCS).
    Type: Application
    Filed: September 8, 2006
    Publication date: October 8, 2009
    Applicant: JAPAN SCIENCE AND TECHNOLOGY AGENCY
    Inventors: Kenta Saito, Hiroshi Sakata, Fumihiko Fujii, Masataka Kinjo, Mamoru Tamura
  • Patent number: 7476545
    Abstract: The present invention provides a method of quickly and accurately detecting and/or assaying an antigen using fluorescence correlation spectroscopy (FCS), which involves a fluorescence-labeled antibody fragment and a non-fluorescence-labeled intact antibody that form a complex with the antigen. There is a significant difference in diffusion rate between the fluorescence-labeled antibody fragment not bonded to the antigen and the complex formed by the the fluorescence-labeled antibody fragment, the antigen, and the non-fluorescence-labeled intact antibody, and this diffusion rate can be determined using FCS. The antigen can be an antigenic protein, such as an abnormal prion or a harmful protein contained in a food material. According to this method, antigens over a wide scope can be assayed regardless of the shape or molecular weight.
    Type: Grant
    Filed: December 4, 2006
    Date of Patent: January 13, 2009
    Assignee: Japan Science and Technology Agency
    Inventors: Masataka Kinjo, Motohiro Horiuchi, Fumihiko Fujii, Hiroshi Sakata, Mamoru Tamura, Masayoshi Ueno, Takayuki Yanagiya
  • Publication number: 20080099728
    Abstract: The present invention provides a water-soluble fluorescent material having a luminescent region at a visible range and having excellent luminescent efficiency. The present invention relates to a water-soluble fluorescent material having a semiconductor nanocrystal; a linear or cyclic phenol compound having hydrophilic group and hydrophobic group that coats at least a portion of the surface of the semiconductor nanocrystal; and a coordinating organic compound coating at least a portion of the surface of the semiconductor nanocrystal.
    Type: Application
    Filed: September 22, 2005
    Publication date: May 1, 2008
    Inventors: Takashi Jin, Masataka Kinjo, Mamoru Tamura, Fumihiko Fujii, Hiroshi Sakata
  • Publication number: 20070292909
    Abstract: An object of the present invention is to provide a red or orange fluorescent protein, which is characterized in that the difference (stokes shift) between an excitation peak value (wavelength of maximum absorption) and a fluorescence peak value (wavelength of maximum fluorescence) is greatened, so that the maximum fluorescence can be obtained by the maximum excitation. The present invention provides a novel fluorescent protein monomerized by introducing a mutation into a florescent protein derived from Fungia sp., and a novel chromoprotein and fluorescent protein derived from Montipora. sp.
    Type: Application
    Filed: December 3, 2004
    Publication date: December 20, 2007
    Inventors: Atsushi Miyawaki, Takako Kogure, Hiroshi Hama, Masataka Kinjo, Kenta Saito, Satoshi Karasawa, Toshio Araki
  • Publication number: 20070154950
    Abstract: The present invention provides a method of quickly and accurately detecting and/or assaying an antigen using fluorescence correlation spectroscopy (FCS), which involves a fluorescence-labeled antibody fragment and a non-fluorescence-labeled intact antibody that form a complex with the antigen. There is a significant difference in diffusion rate between the fluorescence-labeled antibody fragment not bonded to the antigen and the complex formed by the the fluorescence-labeled antibody fragment, the antigen, and the non-fluorescence-labeled intact antibody, and this diffusion rate can be determined using FCS. The antigen can be an antigenic protein, such as an abnormal prion or a harmful protein contained in a food material. According to this method, antigens over a wide scope can be assayed regardless of the shape or molecular weight.
    Type: Application
    Filed: December 4, 2006
    Publication date: July 5, 2007
    Inventors: Masataka Kinjo, Motohiro Horiuchi, Fumihiko Fujii, Hiroshi Sakata, Mamoru Tamura, Masayoshi Ueno, Takayuki Yanagiya
  • Patent number: 7115365
    Abstract: A biological sample containing a target nucleic acid is mixed with a primer, a substrate labeled with fluorescence, and DNA polymerase to prepare a test solution. After a nucleic acid amplification reaction is performed in a predetermined manner, the test solution is attached dropwise on a slide glass, which is mounted on a sample holder of an inverted fluorescence microscope. Data measured by a photomultiplyer are arithmetically operated by applying an autocorrelation function in a data processing apparatus. Based on the arithmetic results, quantification data for the target nucleic acid, are numerically or graphically displayed on a screen of a display apparatus.
    Type: Grant
    Filed: June 3, 1999
    Date of Patent: October 3, 2006
    Assignee: Olympus Optical Co., Ltd.
    Inventor: Masataka Kinjo
  • Publication number: 20020146688
    Abstract: A biological sample containing a target nucleic acid is mixed with a primer, a substrate labeled with fluorescence, and DNA polymerase to prepare a test solution. After a nucleic acid amplification reaction is performed in a predetermined manner, the test solution is attached dropwise on a slide glass, which is mounted on a sample holder of an inverted fluorescence microscope. Data measured by a photomultiplyer are arithmetically operated by applying an autocorrelation function in a data processing apparatus. Based on the arithmetic results, quantification data for the target nucleic acid, are numerically or graphically displayed on a screen of a display apparatus.
    Type: Application
    Filed: June 3, 1999
    Publication date: October 10, 2002
    Applicant: Olympus Optical Co., Ltd.
    Inventor: MASATAKA KINJO