Patents by Inventor Masataka Kinjo
Masataka Kinjo has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Patent number: 11977080Abstract: Disclosed is a method for acquiring information of a target polypeptide, comprising: acquiring a diffusion time of a fluorescently labeled target polypeptide and a diffusion time of each of a plurality of fluorescently labeled reference polypeptides by fluorescence correlation spectroscopy or fluorescence cross-correlation spectroscopy, and acquiring information on size of the fluorescently labeled target polypeptide from the diffusion time of the fluorescently labeled target polypeptide with reference to the diffusion times of the plurality of fluorescently labeled reference polypeptides, wherein information on size of each of the plurality of fluorescently labeled reference polypeptides is known, and the sizes of the plurality of reference polypeptides are different from each other.Type: GrantFiled: April 27, 2021Date of Patent: May 7, 2024Assignees: NATIONAL UNIVERSITY CORPORATION HOKKAIDO UNIVERSITY, SYSMEX CORPORATIONInventors: Masataka Kinjo, Akshay Ganguly, Lausonia Ramaswamy
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Publication number: 20230366819Abstract: Disclosed is a method for obtaining information on von Willebrand factor (VWF), comprising the following steps: denaturing, with urea, VWF contained in a biological sample; fluorescently labeling the denatured VWF using a capturing agent that comprises a fluorescent substance and binds to the denatured VWF; and obtaining information on the size of the fluorescently-labeled VWF by fluorescence correlation spectroscopy or fluorescence cross-correlation spectroscopy.Type: ApplicationFiled: May 10, 2023Publication date: November 16, 2023Applicants: National University Corporation Hokkaido University, SYSMEX CORPORATIONInventors: Masataka KINJO, Akshay GANGULY, Lausonia RAMASWAMY
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Publication number: 20210333287Abstract: Disclosed is a method for acquiring information of a target polypeptide, comprising: acquiring a diffusion time of a fluorescently labeled target polypeptide and a diffusion time of each of a plurality of fluorescently labeled reference polypeptides by fluorescence correlation spectroscopy or fluorescence cross-correlation spectroscopy, and acquiring information on size of the fluorescently labeled target polypeptide from the diffusion time of the fluorescently labeled target polypeptide with reference to the diffusion times of the plurality of fluorescently labeled reference polypeptides, wherein information on size of each of the plurality of fluorescently labeled reference polypeptides is known, and the sizes of the plurality of reference polypeptides are different from each other.Type: ApplicationFiled: April 27, 2021Publication date: October 28, 2021Applicants: NATIONAL UNIVERSITY CORPORATION HOKKAIDO UNIVERSITY, SYSMEX CORPORATIONInventors: Masataka KINJO, Akshay GANGULY, Lausonia RAMASWAMY
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Patent number: 10209504Abstract: Provided is a light detecting device including: a light path branching unit that branches a single detection light path of fluorescence from a specimen, into a plurality of branched light paths; a plurality of light detectors that are provided to the respective branched light paths branched by the light path branching unit and that include an SSPD or Geiger mode APD to detect the fluorescence; and a signal adder that generates a single image signal in accordance with the detection signals outputted from the plurality of light detectors.Type: GrantFiled: March 27, 2018Date of Patent: February 19, 2019Assignees: OLYMPUS CORPORATION, NATIONAL INSTITUTE OF INFORMATION AND COMMUNICATIONS TECHNOLOGY, OSAKA UNIVERSITY, NATIONAL UNIVERSITY CORPORATION HOKKAIDO UNIVERSITYInventors: Hirokazu Kubo, Shigehito Miki, Hirotaka Terai, Taro Yamashita, Tokuko Haraguchi, Yasushi Hiraoka, Masataka Kinjo
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Publication number: 20180284413Abstract: Provided is a light detecting device including: a light path branching unit that branches a single detection light path of fluorescence from a specimen, into a plurality of branched light paths; a plurality of light detectors that are provided to the respective branched light paths branched by the light path branching unit and that include an SSPD or Geiger mode APD to detect the fluorescence; and a signal adder that generates a single image signal in accordance with the detection signals outputted from the plurality of light detectors.Type: ApplicationFiled: March 27, 2018Publication date: October 4, 2018Applicants: OLYMPUS CORPORATION, NATIONAL INSTITUTE OF INFORMATION AND COMMUNICATIONS TECHNOLOGY, OSAKA UNIVERSITY, NATIONAL UNIVERSITY CORPORATION HOKKAIDO UNIVERSITYInventors: Hirokazu KUBO, Shigehito MIKI, Hirotaka TERAI, Taro YAMASHITA, Tokuko HARAGUCHI, Yasushi HIRAOKA, Masataka KINJO
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Patent number: 8420781Abstract: An object of the present invention is to provide a red or orange fluorescent protein, which is characterized in that the difference (stokes shift) between an excitation peak value (wavelength of maximum absorption) and a fluorescence peak value (wavelength of maximum fluorescence) is greatened, so that the maximum fluorescence can be obtained by the maximum excitation. The present invention provides a novel fluorescent protein monomerized by introducing a mutation into a florescent protein derived from Fungia sp., and a novel chromoprotein and fluorescent protein derived from Montipora. sp.Type: GrantFiled: August 2, 2011Date of Patent: April 16, 2013Assignees: Riken, Medical & Biological Laboratories Co., Ltd.Inventors: Atsushi Miyawaki, Takako Kogure, Hiroshi Hama, Masataka Kinjo, Kenta Saito, Satoshi Karasawa, Toshio Araki
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Publication number: 20120034643Abstract: An object of the present invention is to provide a red or orange fluorescent protein, which is characterized in that the difference (stokes shift) between an excitation peak value (wavelength of maximum absorption) and a fluorescence peak value (wavelength of maximum fluorescence) is greatened, so that the maximum fluorescence can be obtained by the maximum excitation. The present invention provides a novel fluorescent protein monomerized by introducing a mutation into a florescent protein derived from Fungia sp., and a novel chromoprotein and fluorescent protein derived from Montipora. sp.Type: ApplicationFiled: August 2, 2011Publication date: February 9, 2012Applicants: MEDICAL & BIOLOGICAL LABORATORIES CO., LTD., RIKENInventors: Atsushi MIYAWAKI, Takako KOGURE, Hiroshi HAMA, Masataka KINJO, Kenta SAITO, Satoshi KARASAWA, Toshio ARAKI
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Patent number: 8017746Abstract: An object of the present invention is to provide a red or orange fluorescent protein, which is characterized in that the difference (stokes shift) between an excitation peak value (wavelength of maximum absorption) and a fluorescence peak value (wavelength of maximum fluorescence) is greatened, so that the maximum fluorescence can be obtained by the maximum excitation. The present invention provides a novel fluorescent protein monomerized by introducing a mutation into a florescent protein derived from Fungia sp., and a novel chromoprotein and fluorescent protein derived from Montipora. sp.Type: GrantFiled: December 3, 2004Date of Patent: September 13, 2011Assignees: Riken, Medical & Biological Laboratories Co., Ltd.Inventors: Atsushi Miyawaki, Takako Kogure, Hiroshi Hama, Masataka Kinjo, Kenta Saito, Satoshi Karasawa, Toshio Araki
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Publication number: 20090253217Abstract: The present invention is to provide a method of quickly detecting an antigen at an arbitrary concentration in an antigen sample, without a multi-stage examination of the concentration ratio between a detection reagent and an antigen to be detected, particularly when the concentration of the antigen in the sample is unknown, in the method of detecting an antigen using fluorescence correlation spectroscopy (FCS) or fluorescence cross-correlation spectroscopy (FCCS).Type: ApplicationFiled: September 8, 2006Publication date: October 8, 2009Applicant: JAPAN SCIENCE AND TECHNOLOGY AGENCYInventors: Kenta Saito, Hiroshi Sakata, Fumihiko Fujii, Masataka Kinjo, Mamoru Tamura
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Patent number: 7476545Abstract: The present invention provides a method of quickly and accurately detecting and/or assaying an antigen using fluorescence correlation spectroscopy (FCS), which involves a fluorescence-labeled antibody fragment and a non-fluorescence-labeled intact antibody that form a complex with the antigen. There is a significant difference in diffusion rate between the fluorescence-labeled antibody fragment not bonded to the antigen and the complex formed by the the fluorescence-labeled antibody fragment, the antigen, and the non-fluorescence-labeled intact antibody, and this diffusion rate can be determined using FCS. The antigen can be an antigenic protein, such as an abnormal prion or a harmful protein contained in a food material. According to this method, antigens over a wide scope can be assayed regardless of the shape or molecular weight.Type: GrantFiled: December 4, 2006Date of Patent: January 13, 2009Assignee: Japan Science and Technology AgencyInventors: Masataka Kinjo, Motohiro Horiuchi, Fumihiko Fujii, Hiroshi Sakata, Mamoru Tamura, Masayoshi Ueno, Takayuki Yanagiya
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Publication number: 20080099728Abstract: The present invention provides a water-soluble fluorescent material having a luminescent region at a visible range and having excellent luminescent efficiency. The present invention relates to a water-soluble fluorescent material having a semiconductor nanocrystal; a linear or cyclic phenol compound having hydrophilic group and hydrophobic group that coats at least a portion of the surface of the semiconductor nanocrystal; and a coordinating organic compound coating at least a portion of the surface of the semiconductor nanocrystal.Type: ApplicationFiled: September 22, 2005Publication date: May 1, 2008Inventors: Takashi Jin, Masataka Kinjo, Mamoru Tamura, Fumihiko Fujii, Hiroshi Sakata
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Publication number: 20070292909Abstract: An object of the present invention is to provide a red or orange fluorescent protein, which is characterized in that the difference (stokes shift) between an excitation peak value (wavelength of maximum absorption) and a fluorescence peak value (wavelength of maximum fluorescence) is greatened, so that the maximum fluorescence can be obtained by the maximum excitation. The present invention provides a novel fluorescent protein monomerized by introducing a mutation into a florescent protein derived from Fungia sp., and a novel chromoprotein and fluorescent protein derived from Montipora. sp.Type: ApplicationFiled: December 3, 2004Publication date: December 20, 2007Inventors: Atsushi Miyawaki, Takako Kogure, Hiroshi Hama, Masataka Kinjo, Kenta Saito, Satoshi Karasawa, Toshio Araki
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Publication number: 20070154950Abstract: The present invention provides a method of quickly and accurately detecting and/or assaying an antigen using fluorescence correlation spectroscopy (FCS), which involves a fluorescence-labeled antibody fragment and a non-fluorescence-labeled intact antibody that form a complex with the antigen. There is a significant difference in diffusion rate between the fluorescence-labeled antibody fragment not bonded to the antigen and the complex formed by the the fluorescence-labeled antibody fragment, the antigen, and the non-fluorescence-labeled intact antibody, and this diffusion rate can be determined using FCS. The antigen can be an antigenic protein, such as an abnormal prion or a harmful protein contained in a food material. According to this method, antigens over a wide scope can be assayed regardless of the shape or molecular weight.Type: ApplicationFiled: December 4, 2006Publication date: July 5, 2007Inventors: Masataka Kinjo, Motohiro Horiuchi, Fumihiko Fujii, Hiroshi Sakata, Mamoru Tamura, Masayoshi Ueno, Takayuki Yanagiya
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Patent number: 7115365Abstract: A biological sample containing a target nucleic acid is mixed with a primer, a substrate labeled with fluorescence, and DNA polymerase to prepare a test solution. After a nucleic acid amplification reaction is performed in a predetermined manner, the test solution is attached dropwise on a slide glass, which is mounted on a sample holder of an inverted fluorescence microscope. Data measured by a photomultiplyer are arithmetically operated by applying an autocorrelation function in a data processing apparatus. Based on the arithmetic results, quantification data for the target nucleic acid, are numerically or graphically displayed on a screen of a display apparatus.Type: GrantFiled: June 3, 1999Date of Patent: October 3, 2006Assignee: Olympus Optical Co., Ltd.Inventor: Masataka Kinjo
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Publication number: 20020146688Abstract: A biological sample containing a target nucleic acid is mixed with a primer, a substrate labeled with fluorescence, and DNA polymerase to prepare a test solution. After a nucleic acid amplification reaction is performed in a predetermined manner, the test solution is attached dropwise on a slide glass, which is mounted on a sample holder of an inverted fluorescence microscope. Data measured by a photomultiplyer are arithmetically operated by applying an autocorrelation function in a data processing apparatus. Based on the arithmetic results, quantification data for the target nucleic acid, are numerically or graphically displayed on a screen of a display apparatus.Type: ApplicationFiled: June 3, 1999Publication date: October 10, 2002Applicant: Olympus Optical Co., Ltd.Inventor: MASATAKA KINJO