Patents by Inventor Masataka Shirai
Masataka Shirai has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
-
Publication number: 20230288301Abstract: A microparticle filling method of the present disclosure is a method of filling at least one or more containers with microparticles, including: sucking a suspension of the microparticles into a nozzle; concentrating the microparticles in the suspension to form a high-concentration suspension having a predetermined microparticle concentration at a tip of the nozzle; bringing the high-concentration suspension in contact with an inner wall of the container; and separating the nozzle from the container after the contact to fill the container with the high-concentration suspension.Type: ApplicationFiled: March 9, 2020Publication date: September 14, 2023Inventors: Masataka SHIRAI, Tomoyuki SAKAI
-
Patent number: 11680286Abstract: The present invention relates to a method, a device, and an apparatus for analyzing the expression of a gene in single cells. Specifically, the present invention relates to: a device for gene expression analysis, characterized by including a support, in which a nucleic acid probe having a test nucleic acid capture sequence and a known sequence, and further containing a cell recognition tag sequence which differs depending on the difference in position on the surface of the support or in the vicinity of the surface thereof, and a common primer sequence having a known sequence is two-dimensionally distributed and immobilized on the surface of the support or in the vicinity of the surface thereof; and a method and an apparatus using the device for gene expression analysis.Type: GrantFiled: May 18, 2021Date of Patent: June 20, 2023Assignee: Hitachi, Ltd.Inventors: Masataka Shirai, Hideki Kambara, Kiyomi Taniguchi, Maiko Tanabe
-
Patent number: 11454577Abstract: Provided is, in a sample of a cell or a micro region at a position designated in a microscope image of a tissue section, an apparatus and a method for analyzing a biomolecule, which are capable of collecting and analyzing a biomolecule in a single cell or in a micro region without damaging surrounding cells.Type: GrantFiled: October 3, 2018Date of Patent: September 27, 2022Assignee: HITACHI, LTD.Inventor: Masataka Shirai
-
Patent number: 11446662Abstract: A system for capturing a biomolecule in a single cell includes: a two-dimensional array having single cell capture holes on one surface of a substrate, and biomolecule capture areas inside the substrate each comprising a biomolecule capture member for capturing a biomolecule extracted from individual cells respectively captured by the single cell capture holes; a flow channel for flowing a sample containing cells to be assayed, from a 1st direction parallel to the surface of the substrate; a structure on the surface of the substrate and opposed to the 1st direction on the downstream side of each of the single cell capture holes; a 1st application means applying a 1st flow, and a 1st control means; and a 2nd application means applying a 2nd flow orthogonal to the one surface of the substrate toward each biomolecule capture area from each corresponding single cell capture hole, and a 2nd control means.Type: GrantFiled: March 28, 2016Date of Patent: September 20, 2022Assignee: HITACHI, LTD.Inventors: Tomoyuki Sakai, Masataka Shirai
-
Patent number: 11299727Abstract: A device is provided for capturing a nucleic acid in a single cell, having: a two-dimensional array comprising a substrate, a plurality of single cell capturing holes provided on one surface of the substrate, and a nucleic acid capturing region comprising, on the inside of the substrate, a nucleic acid capturing body, which is configured to capture the nucleic acids extracted from the individual cells respectively captured by the single cell capturing hole; a flow channel which is provided adjacent to the nucleic acid capturing region of the substrate, and is configured to discharge a solution in the nucleic acid capturing region; and a cylindrical structure body which is arranged on the substrate at the time of introducing a cell suspension and encloses a plurality of the single cell capturing holes, wherein the cylindrical structure body is removed after the cells are captured by the single cell capturing holes.Type: GrantFiled: March 6, 2017Date of Patent: April 12, 2022Assignee: HITACHI, LTD.Inventors: Tomoyuki Sakai, Masataka Shirai
-
Patent number: 11214796Abstract: The present invention provides a method and/or means for collecting and analyzing an individual cell in a tissue, and at the same time, quantitatively monitoring the expression levels of various genes while keeping two-dimensional information in the tissue. Specifically, the present invention provides a method comprising preparing a cDNA library from mRNA while keeping two-dimensional cellular distribution information and obtaining the gene expression levels at any site or all sites at a level of single cell. More specifically, the present invention provides a method comprising preparing a cDNA library in a sheet-form from mRNA while keeping two-dimensional cellular distribution information and repeatedly using the cDNA library in the detection of the gene expression, thereby allowing measurement of the expression distribution for a number of genes at a high accuracy.Type: GrantFiled: June 13, 2017Date of Patent: January 4, 2022Assignee: HITACHI, LTD.Inventors: Masataka Shirai, Hideki Kambara, Kiyomi Taniguchi
-
Publication number: 20210388422Abstract: The present invention relates to a method, a device, and an apparatus for analyzing the expression of a gene in single cells. Specifically, the present invention relates to: a device for gene expression analysis, characterized by including a support, in which a nucleic acid probe having a test nucleic acid capture sequence and a known sequence, and further containing a cell recognition tag sequence which differs depending on the difference in position on the surface of the support or in the vicinity of the surface thereof, and a common primer sequence having a known sequence is two-dimensionally distributed and immobilized on the surface of the support or in the vicinity of the surface thereof; and a method and an apparatus using the device for gene expression analysis.Type: ApplicationFiled: May 18, 2021Publication date: December 16, 2021Inventors: Masataka SHIRAI, Hideki KAMBARA, Kiyomi TANIGUCHI, Maiko TANABE
-
Patent number: 11053536Abstract: The present invention relates to a method, a device, and an apparatus for analyzing the expression of a gene in single cells. Specifically, the present invention relates to: a device for gene expression analysis, characterized by including a support, in which a nucleic acid probe having a test nucleic acid capture sequence and a known sequence, and further containing a cell recognition tag sequence which differs depending on the difference in position on the surface of the support or in the vicinity of the surface thereof, and a common primer sequence having a known sequence is two-dimensionally distributed and immobilized on the surface of the support or in the vicinity of the surface thereof; and a method and an apparatus using the device for gene expression analysis.Type: GrantFiled: July 30, 2012Date of Patent: July 6, 2021Assignee: Hitachi, Ltd.Inventors: Masataka Shirai, Hideki Kambara, Kiyomi Taniguchi, Maiko Tanabe
-
Patent number: 11041760Abstract: In multiplex CARS, a fingerprint region is a region where signals are densely concentrated. Information about the intensities and spatial distribution of these signals is important in cell analysis. However, there has been a problem in that the signal intensities are low. Accordingly, mechanisms 702 and 703 for adjusting the power branching ratio between light that enters a photonic crystal fiber 705 and pumping light; mechanisms 710 and 711 for adjusting the divergence/convergence state of the pumping light; and mechanisms 706 and 2101 for adjusting the divergence/convergence state of Stokes light are provided to enable adjustment for emphasizing a desired wavelength band.Type: GrantFiled: March 11, 2015Date of Patent: June 22, 2021Assignee: HITACHI HIGH-TECH CORPORATIONInventors: Manabu Shiozawa, Koichi Watanabe, Masataka Shirai
-
Publication number: 20210181076Abstract: Provided is, in a sample of a cell or a micro region at a position designated in a microscope image of a tissue section, an apparatus and a method for analyzing a biomolecule, which are capable of collecting and analyzing a biomolecule in a single cell or in a micro region without damaging surrounding cells.Type: ApplicationFiled: October 3, 2018Publication date: June 17, 2021Applicant: HITACHI, LTD.Inventor: Masataka SHIRAI
-
Patent number: 10876106Abstract: In order to interpret an arbitrary sequence region in many genes in many cells, it is necessary to degrade a nucleic acid into fragments and introduce a sequence that is different from one cell to another into each of the fragments. However, in the conventional configuration for analyzing many cells, there has been a problem that mixing of the degraded fragments among areas occurs before a tag sequence unique for each of the areas is introduced. The present invention provides a system for capturing a nucleic acid extracted from a cell in each of plural areas on a substrate and synthesizing a complementary DNA (cDNA) of the nucleic acid for each of the areas, wherein the system also includes a means for immediately introducing a tag sequence unique for each of the areas to the reaction product.Type: GrantFiled: April 9, 2015Date of Patent: December 29, 2020Assignee: HITACHI, LTD.Inventors: Maiko Tanabe, Masataka Shirai, Tomoyuki Sakai
-
Publication number: 20200385712Abstract: A method for analyzing gene expression in a cell using a device, the method including: introducing a plurality of cells into microreactors so that a single cell corresponds to each microreactor, capturing mRNA from the single cell on the probe, synthesizing first cDNA by subjecting the captured mRNA to a reverse transcription reaction, to produce a first cDNA library derived from the single cell on the solid supports, washing the solid supports, a step of synthesizing second cDNA from the first cDNA library, performing fragmentation of double-stranded DNA containing the first and second cDNA and addition of a tag sequence, removing a component other than an immobilized double-stranded DNA fragment by washing the solid supports with a washing solution, performing amplification of the double-stranded DNA fragment, and performing, for the amplified sequence, analysis of gene expression in each single cell, using the cell identification sequence and the molecule identification sequence.Type: ApplicationFiled: November 9, 2018Publication date: December 10, 2020Applicant: HITACHI, LTD.Inventors: Kiyomi TANIGUCHI, Masataka SHIRAI
-
Publication number: 20200354781Abstract: The purpose of the present invention is to provide a single cell analysis device in which the improvement of the nucleic acid capturing efficiency and the improvement of the cell capturing efficiency are both achieved and a highly accurate single cell analysis data is thereby obtained. The present invention relates to an improvement of a cell analysis device including a two-dimensional array chip having a plurality of cell capture parts capable of capturing a single cell in each of the capture parts, and nucleic acid capture parts corresponding to the respective cell capture parts, the nucleic acid capture parts being capable of capturing a nucleic acid extracted from the cell captured by the cell capture part.Type: ApplicationFiled: July 28, 2020Publication date: November 12, 2020Inventors: Masataka SHIRAI, Tomoyuki SAKAI, Kenko UCHIDA
-
Patent number: 10752941Abstract: The purpose of the present invention is to provide a single cell analysis device in which the improvement of the nucleic acid capturing efficiency and the improvement of the cell capturing efficiency are both achieved and a highly accurate single cell analysis data is thereby obtained. The present invention relates to an improvement of a cell analysis device including a two-dimensional array chip having a plurality of cell capture parts capable of capturing a single cell in each of the capture parts, and nucleic acid capture parts corresponding to the respective cell capture parts, the nucleic acid capture parts being capable of capturing a nucleic acid extracted from the cell captured by the cell capture part.Type: GrantFiled: December 1, 2015Date of Patent: August 25, 2020Assignee: HITACHI HIGH-TECH CORPORATIONInventors: Masataka Shirai, Tomoyuki Sakai, Kenko Uchida
-
Patent number: 10646869Abstract: (1) Plural reaction chambers each including at least one cell capturing unit and at least one nucleic acid capturing unit connected via a flow channel, (2) a first liquid flow channel commonly connected to the plural reaction chambers on a first surface side, (3) plural second liquid flow channels connected to the plural reaction chambers in a one-on-one relationship on the first surface side, and (4) a third liquid flow channel commonly connected to the plural reaction chambers on a second surface side opposite to the first surface, are provided in a flow cell device for single cell analysis.Type: GrantFiled: February 3, 2015Date of Patent: May 12, 2020Assignee: Hitachi, Ltd.Inventors: Masataka Shirai, Tomoyuki Sakai
-
Publication number: 20190194645Abstract: A device is provided for capturing a nucleic acid in a single cell, having: a two-dimensional array comprising a substrate, a plurality of single cell capturing holes provided on one surface of the substrate, and a nucleic acid capturing region comprising, on the inside of the substrate, a nucleic acid capturing body, which is configured to capture the nucleic acids extracted from the individual cells respectively captured by the single cell capturing hole; a flow channel which is provided adjacent to the nucleic acid capturing region of the substrate, and is configured to discharge a solution in the nucleic acid capturing region; and a cylindrical structure body which is arranged on the substrate at the time of introducing a cell suspension and encloses a plurality of the single cell capturing holes, wherein the cylindrical structure body is removed after the cells are captured by the single cell capturing holes.Type: ApplicationFiled: March 6, 2017Publication date: June 27, 2019Applicant: Hitachi ,Ltd.Inventors: Tomoyuki SAKAI, Masataka SHIRAI
-
Publication number: 20180304262Abstract: (1) Plural reaction chambers each including at least one cell capturing unit and at least one nucleic acid capturing unit connected via a flow channel, (2) a first liquid flow channel commonly connected to the plural reaction chambers on a first surface side, (3) plural second liquid flow channels connected to the plural reaction chambers in a one-on-one relationship on the first surface side, and (4) a third liquid flow channel commonly connected to the plural reaction chambers on a second surface side opposite to the first surface, are provided in a flow cell device for single cell analysis.Type: ApplicationFiled: February 3, 2015Publication date: October 25, 2018Inventors: Masataka SHIRAI, Tomoyuki SAKAI
-
Publication number: 20180272349Abstract: A system for capturing a biomolecule in a single cell includes: a two-dimensional array having single cell capture holes on one surface of a substrate, and biomolecule capture areas inside the substrate each comprising a biomolecule capture member for capturing a biomolecule extracted from individual cells respectively captured by the single cell capture holes; a flow channel for flowing a sample containing cells to be assayed, from a 1st direction parallel to the surface of the substrate; a structure on the surface of the substrate and opposed to the 1st direction on the downstream side of each of the single cell capture holes; a 1st application means applying a 1st flow, and a 1st control means; and a 2nd application means applying a 2nd flow orthogonal to the one surface of the substrate toward each biomolecule capture area from each corresponding single cell capture hole, and a 2nd control means.Type: ApplicationFiled: March 28, 2016Publication date: September 27, 2018Applicant: Hitachi, Ltd.Inventors: Tomoyuki SAKAI, Masataka SHIRAI
-
Publication number: 20180265918Abstract: The purpose of the present invention is to provide a single cell analysis device in which the improvement of the nucleic acid capturing efficiency and the improvement of the cell capturing efficiency are both achieved and a highly accurate single cell analysis data is thereby obtained. The present invention relates to an improvement of a cell analysis device including a two-dimensional array chip having a plurality of cell capture parts capable of capturing a single cell in each of the capture parts, and nucleic acid capture parts corresponding to the respective cell capture parts, the nucleic acid capture parts being capable of capturing a nucleic acid extracted from the cell captured by the cell capture part.Type: ApplicationFiled: December 1, 2015Publication date: September 20, 2018Inventors: Masataka SHIRAI, Tomoyuki SAKAI, Kenko UCHIDA
-
Patent number: 10030240Abstract: In order to conduct gene expression analysis of a number of genes in a number of cells, it has been necessary to separate cells, extract genes therefrom, amplify nucleic acids, and perform sequence analysis. However, separation of cells imposes damages on the cells, and it requires the use of an expensive system. Gene expression analysis in each cell can be carried out with high accuracy by arranging a pair of structures comprising a cell trapping section and a nucleic acid trapping section in a vertical direction to extract individual genes in relevant cells, synthesizing cDNA in the nucleic acid trapping section, amplifying nucleic acids, and analyzing the sequences using a next-generation sequencer.Type: GrantFiled: March 12, 2013Date of Patent: July 24, 2018Assignee: Hitachi, Ltd.Inventors: Masataka Shirai, Hideki Kambara, Kiyomi Taniguchi, Maiko Tanabe