Abstract: Pluripotent stem cells are suspension-cultured with the undifferentiated state thereof maintained. In suspension culture of pluripotent stem cells, the undifferentiated state is maintained by the presence of a PKC inhibitor, especially, a PKC? inhibitor, and a tankyrase inhibitor (TNKS inhibitor).

Abstract: The present application provides: a suppressor of pluripotent stem cell aggregation for use in suspension culture of pluripotent stem cells, comprising a cell cycle arresting agent; a method for producing a pluripotent stem cell aggregate(s), comprising a step of subjecting pluripotent stem cells to suspension culture in a culture medium comprising the suppressor; a pluripotent stem cell aggregate obtained by the method; and a composition for culture of pluripotent stem cells comprising a cell aggregate(s) of pluripotent stem cells, a culture medium, and a cell cycle arresting agent. According to the method, it is possible, in suspension culture of pluripotent stem cells, to produce a cell aggregate(s) with high cell viability and appropriate size in a high yield while maintaining the undifferentiated state of the cells and suppressing the aggregation.

Abstract: A method for producing pluripotent stem cells includes a step of performing suspension culture of pluripotent stem cells under a condition in which an amount, which is calculated by the following Equation (1), of WNT protein contained in a unit of a medium in contact with a unit area of a cell surface of a pluripotent stem cell is maintained at 2.9×102 ?g/mL·cm2 or less.

Abstract: A production method for a cell population containing pluripotent stem cells includes: a step of culturing cell populations containing pluripotent stem cells; and a step of sorting out a cell population having the following characteristics (a) and (b) from the cell populations containing pluripotent stem cells. (a) A relative expression level of a LEF1 gene with respect to an expression level of a ?-actin gene is 5.5×10?4 or less in the cell population. (b) A percentage of cells positive for LEF1 is 55% or less in the cell population.

Abstract: A portable, handheld game console includes a main body incorporating a touch-sensitive display screen and a variety of input devices. The input devices can include shoulder buttons located on a peripheral side surface of the main body, as well as input devices located on the main body adjacent the touch-sensitive display screen.

Abstract: An object of the present invention is to provide an endodermal cell population for obtaining optimal somatic cells as cell therapy preparations. The endodermal cell population of the present invention has a reduced content proportion of undifferentiated cells in the cell population and contains endodermal cells differentiable into optimal somatic cells as cell therapy preparations. Further, a somatic cell derived from the endodermal cell population of the present invention has excellent therapeutic effects as a cell therapy preparation.

Abstract: A problem addressed by the present invention is to provide a method for producing pancreatic ? cells from endothermal cells that have been induced to differentiate from pluripotent stein cells, wherein the method allows the production of pancreatic ? cells of high quality, and to provide pancreatic progenitor (PP) cells, pancreatic endocrine precursor (EP) cells, and pancreatic ? cells produced by the above-mentioned method.

Abstract: A problem addressed by the present invention is to provide a method for producing primitive gut tube cells from endothermal cells that have been induced to differentiate from pluripotent stein cells, wherein the method allows the production of pancreatic ? cells of high quality. The present invention provides a method for producing primitive gut tube (PGT) cells comprising a step of culturing, in the absence of a bone morphogenetic protein (BMP) signaling inhibitor, endodermal cells that have been induced to differentiate from pluripotent stem cells.

Abstract: A method for producing cell aggregates includes culturing cells while suspending the cells in a liquid culture medium comprising a lysophospholipid. A composition includes a lysophospholipid, wherein the composition is a liquid culture medium or a composition added to a liquid culture medium.

Abstract: The present invention is directed to providing a means for appropriately controlling the size of cell aggregates without relying on mechanical/physical means. Specifically, the present invention relates to a cell aggregation promoter for use in suspension culture of cells, comprising an SRF inhibitor. The present invention also relates to a method for producing cell aggregates, comprising a step of culturing cells in suspension in a culture medium comprising an SRF inhibitor.

Abstract: Provided is a means for appropriately controlling the size of cell aggregates without relying on mechanical/physical means. The present invention relates to a cell aggregation suppressor for use in suspension culture of cells, comprising an agonist of thrombin receptor. The present invention also relates to a method for producing cell aggregates, comprising a step of culturing cells in suspension in a culture medium comprising an agonist of thrombin receptor.

Abstract: A portable, handheld game console includes a main body incorporating a touch-sensitive display screen and a variety of input devices. The input devices can include shoulder buttons located on a peripheral side surface of the main body, as well as input devices located on the main body adjacent the touch-sensitive display screen.

Abstract: A portable, handheld game console includes a main body incorporating a touch-sensitive display screen and a variety of input devices. The input devices can include shoulder buttons located on a peripheral side surface of the main body, as well as input devices located on the main body adjacent the touch-sensitive display screen.

Abstract: A portable, handheld game console includes a main body incorporating a touch-sensitive display screen and a variety of input devices. The input devices can include shoulder buttons located on a peripheral side surface of the main body, as well as input devices located on the main body adjacent the touch-sensitive display screen.

Abstract: A portable, handheld game console includes a main body incorporating a touch-sensitive display screen and a variety of input devices. The input devices can include shoulder buttons located on a peripheral side surface of the main body, as well as input devices located on the main body adjacent the touch-sensitive display screen.

Abstract: An example terminal device includes a generally plate-shaped housing, an LCD provided on the front side of the housing, analog sticks, and first and second buttons. The analog sticks are provided respectively in left and right portions of the LCD above the center of the housing. The first and second buttons are provided on the back side of the housing so as to generally correspond to the analog sticks on the front side of the housing.

Abstract: A method for producing cell aggregates includes culturing cells while suspending the cells in a liquid culture medium comprising a lysophospholipid. A composition includes a lysophospholipid, wherein the composition is a liquid culture medium or a composition added to a liquid culture medium.

Abstract: A controller cover includes a main body portion attached to an outer surface of a controller, and the main body portion includes a contacted portion where a contacted inner surface is in contact with the outer surface of the controller and a non-contacted portion where a non-contacted inner surface is not in contact with the outer surface of the controller. Elastic deforming protrusions are formed on the non-contacted inner surface. When the controller attached with the cover interferes with other thing, and the non-contacted portion of the cover is subjected to shock, the elastic deforming protrusion of the non-contacted portion is deformed to ease the shock, so that the controller and the interfering other thing are protected.